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The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches.
Proc Natl Acad Sci U S A. 1996 Jan 09; 93(1):279-83.PN

Abstract

We investigated the role of the Salmonella typhimurium fimbrial operon formed by the genes lpfABCDE in infection of mice. A mutant in lpfC, the gene encoding the fimbrial outer membrane usher, had an approximately 5-fold increased 50% lethal dose when administered orally to mice. When mice were infected with a mixture of the lpfC mutant and isogenic wild-type S. typhimurium, the lpfC mutant was recovered in lower numbers from Peyer's patches, mesenteric lymph nodes, liver, and spleen. In an organ culture model using murine intestinal loops, lpfC mutants were shown to be associated in lower numbers than wild-type bacteria with Peyer's patches but not with villous intestine. The defect of the lpfC mutant in adhesion to Peyer's patches could be complemented by introducing lpfABCDE on a cosmid. Similarly, heterologous expression of the Salmonella lpf operon in Escherichia coli resulted in an increased adhesion to histological thin sections of Peyer's patch lymph follicles. Electron microscopic analysis of histological sections taken from Peyer's patches after intragastric infection of mice showed that, in contrast to the S. typhimurium wild type, the isogenic lpfC mutant did not destroy M cells of the follicle-associated epithelium. These data show that the Salmonella lpf operon is involved in adhesion to murine Peyer's patches.

Authors+Show Affiliations

Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8552622

Citation

Bäumler, A J., et al. "The Lpf Fimbrial Operon Mediates Adhesion of Salmonella Typhimurium to Murine Peyer's Patches." Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 1, 1996, pp. 279-83.
Bäumler AJ, Tsolis RM, Heffron F. The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches. Proc Natl Acad Sci U S A. 1996;93(1):279-83.
Bäumler, A. J., Tsolis, R. M., & Heffron, F. (1996). The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches. Proceedings of the National Academy of Sciences of the United States of America, 93(1), 279-83.
Bäumler AJ, Tsolis RM, Heffron F. The Lpf Fimbrial Operon Mediates Adhesion of Salmonella Typhimurium to Murine Peyer's Patches. Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):279-83. PubMed PMID: 8552622.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The lpf fimbrial operon mediates adhesion of Salmonella typhimurium to murine Peyer's patches. AU - Bäumler,A J, AU - Tsolis,R M, AU - Heffron,F, PY - 1996/1/9/pubmed PY - 1996/1/9/medline PY - 1996/1/9/entrez SP - 279 EP - 83 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 93 IS - 1 N2 - We investigated the role of the Salmonella typhimurium fimbrial operon formed by the genes lpfABCDE in infection of mice. A mutant in lpfC, the gene encoding the fimbrial outer membrane usher, had an approximately 5-fold increased 50% lethal dose when administered orally to mice. When mice were infected with a mixture of the lpfC mutant and isogenic wild-type S. typhimurium, the lpfC mutant was recovered in lower numbers from Peyer's patches, mesenteric lymph nodes, liver, and spleen. In an organ culture model using murine intestinal loops, lpfC mutants were shown to be associated in lower numbers than wild-type bacteria with Peyer's patches but not with villous intestine. The defect of the lpfC mutant in adhesion to Peyer's patches could be complemented by introducing lpfABCDE on a cosmid. Similarly, heterologous expression of the Salmonella lpf operon in Escherichia coli resulted in an increased adhesion to histological thin sections of Peyer's patch lymph follicles. Electron microscopic analysis of histological sections taken from Peyer's patches after intragastric infection of mice showed that, in contrast to the S. typhimurium wild type, the isogenic lpfC mutant did not destroy M cells of the follicle-associated epithelium. These data show that the Salmonella lpf operon is involved in adhesion to murine Peyer's patches. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/8552622/The_lpf_fimbrial_operon_mediates_adhesion_of_Salmonella_typhimurium_to_murine_Peyer's_patches_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=8552622 DB - PRIME DP - Unbound Medicine ER -