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[Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions].
Rev Argent Microbiol. 1995 Apr-Jun; 27(2):91-8.RA

Abstract

The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula.

Authors+Show Affiliations

Pujol CA
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Coto CE
No affiliation info available
Damonte EB
No affiliation info available

MeSH

AnimalsAntiviral AgentsCarcinoma, Squamous CellCell LineCell SurvivalChlorocebus aethiopsCricetinaeDrug Evaluation, PreclinicalHumansKidneyMannansSeaweedSimplexvirusSkin NeoplasmsTumor Cells, CulturedVero CellsViral Plaque AssayVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

8552762

Citation

Pujol, C A., et al. "[Determination of the Antiviral Activity of a Naturally Occurring Sulfated Xylomannan Under Various Experimental Conditions]." Revista Argentina De Microbiologia, vol. 27, no. 2, 1995, pp. 91-8.
Pujol CA, Coto CE, Damonte EB. [Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions]. Rev Argent Microbiol. 1995;27(2):91-8.
Pujol, C. A., Coto, C. E., & Damonte, E. B. (1995). [Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions]. Revista Argentina De Microbiologia, 27(2), 91-8.
Pujol CA, Coto CE, Damonte EB. [Determination of the Antiviral Activity of a Naturally Occurring Sulfated Xylomannan Under Various Experimental Conditions]. Rev Argent Microbiol. 1995 Apr-Jun;27(2):91-8. PubMed PMID: 8552762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions]. AU - Pujol,C A, AU - Coto,C E, AU - Damonte,E B, PY - 1995/4/1/pubmed PY - 1995/4/1/medline PY - 1995/4/1/entrez SP - 91 EP - 8 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 27 IS - 2 N2 - The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/8552762/[Determination_of_the_antiviral_activity_of_a_naturally_occurring_sulfated_xylomannan_under_various_experimental_conditions]_ DB - PRIME DP - Unbound Medicine ER -