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Cis-acting elements of the encephalomyocarditis virus internal ribosomal entry site.
Virology. 1995 Dec 20; 214(2):660-3.V

Abstract

Translation initiation of encephalomyocarditis virus (EMCV) mRNA occurs by ribosomal entry into the 5' untranslated region. Internal ribosome binding to EMCV mRNA requires a viral cis-acting element, termed the internal ribosomal entry site (IRES), and cellular trans-acting factors. The polypyrimidine tract binding protein (PTB) has been identified as one such trans-acting factor required for EMCV IRES-dependent translation. Using a dicistronic mRNA and an in vitro translation system, we have identified cis-acting elements of the EMCV IRES required for IRES-dependent translation. The results identify several regions of the IRES that are required for efficient IRES-dependent translation, including the PTB binding site. Other regions of the IRES may act only as spacer sequences, analogous to the spacer sequences found in rhinovirus and enterovirus IRES elements, to link essential regions of the IRES together. The flexibility of one region of the EMCV IRES was demonstrated by an insertion of 125 nucleotides that had little effect on IRES function while the constraint imposed on another region was demonstrated by a 3-nucleotide deletion that nearly abolished IRES-dependent translation.

Authors+Show Affiliations

RiboGene, Inc., Hayward, California 94545, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8553572

Citation

Witherell, G W., et al. "Cis-acting Elements of the Encephalomyocarditis Virus Internal Ribosomal Entry Site." Virology, vol. 214, no. 2, 1995, pp. 660-3.
Witherell GW, Schultz-Witherell CS, Wimmer E. Cis-acting elements of the encephalomyocarditis virus internal ribosomal entry site. Virology. 1995;214(2):660-3.
Witherell, G. W., Schultz-Witherell, C. S., & Wimmer, E. (1995). Cis-acting elements of the encephalomyocarditis virus internal ribosomal entry site. Virology, 214(2), 660-3.
Witherell GW, Schultz-Witherell CS, Wimmer E. Cis-acting Elements of the Encephalomyocarditis Virus Internal Ribosomal Entry Site. Virology. 1995 Dec 20;214(2):660-3. PubMed PMID: 8553572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cis-acting elements of the encephalomyocarditis virus internal ribosomal entry site. AU - Witherell,G W, AU - Schultz-Witherell,C S, AU - Wimmer,E, PY - 1995/12/20/pubmed PY - 1995/12/20/medline PY - 1995/12/20/entrez SP - 660 EP - 3 JF - Virology JO - Virology VL - 214 IS - 2 N2 - Translation initiation of encephalomyocarditis virus (EMCV) mRNA occurs by ribosomal entry into the 5' untranslated region. Internal ribosome binding to EMCV mRNA requires a viral cis-acting element, termed the internal ribosomal entry site (IRES), and cellular trans-acting factors. The polypyrimidine tract binding protein (PTB) has been identified as one such trans-acting factor required for EMCV IRES-dependent translation. Using a dicistronic mRNA and an in vitro translation system, we have identified cis-acting elements of the EMCV IRES required for IRES-dependent translation. The results identify several regions of the IRES that are required for efficient IRES-dependent translation, including the PTB binding site. Other regions of the IRES may act only as spacer sequences, analogous to the spacer sequences found in rhinovirus and enterovirus IRES elements, to link essential regions of the IRES together. The flexibility of one region of the EMCV IRES was demonstrated by an insertion of 125 nucleotides that had little effect on IRES function while the constraint imposed on another region was demonstrated by a 3-nucleotide deletion that nearly abolished IRES-dependent translation. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/8553572/Cis_acting_elements_of_the_encephalomyocarditis_virus_internal_ribosomal_entry_site_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(85)70081-5 DB - PRIME DP - Unbound Medicine ER -