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A rapid and inexpensive method for processing induced sputum for detection of Pneumocystis carinii.
Am J Clin Pathol. 1996 Jan; 105(1):52-7.AJ

Abstract

The authors describe a method to process induced sputum specimens for detection of Pneumocystis carinii which is simple, rapid and inexpensive. Induced sputum and bronchoalveolar lavage (BAL) were obtained within a 24-hour period from 41 patients who were HIV-positive and had pulmonary symptoms suspicious for P carinii pneumonia. Induced sputum or BAL fluid was placed into Saccomanno's fixative, blended, and centrifuged. The sediment was stained for P carinii cysts by a modified method with Fungi-Fluor Solution A (Polysciences, Warington, PA) and the Genetic Systems Pneumocystis carinii Immunofluorescence Antibody (Genetic Systems, Seattle, WA). The Genetic Systems stain on the BAL specimen was positive in 35 patients and was the standard for comparison. With a single induced sputum, the Genetic Systems stain detected 31 (89%) positive patients, whereas the Fungi-Fluor stain detected 21 (60%). The sensitivity for detecting P carinii cysts in induced sputum was significantly greater (P < 0.05) for the Genetic Systems stain.

Authors+Show Affiliations

Department of Pulmonary Medicine, Ben Taub General Hospital, Houston, TX 77030, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8561088

Citation

Rocha, P, et al. "A Rapid and Inexpensive Method for Processing Induced Sputum for Detection of Pneumocystis Carinii." American Journal of Clinical Pathology, vol. 105, no. 1, 1996, pp. 52-7.
Rocha P, Awe RJ, Guy ES, et al. A rapid and inexpensive method for processing induced sputum for detection of Pneumocystis carinii. Am J Clin Pathol. 1996;105(1):52-7.
Rocha, P., Awe, R. J., Guy, E. S., Gaines, V., Banez, E. I., Stager, C. E., & Guntupalli, K. K. (1996). A rapid and inexpensive method for processing induced sputum for detection of Pneumocystis carinii. American Journal of Clinical Pathology, 105(1), 52-7.
Rocha P, et al. A Rapid and Inexpensive Method for Processing Induced Sputum for Detection of Pneumocystis Carinii. Am J Clin Pathol. 1996;105(1):52-7. PubMed PMID: 8561088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid and inexpensive method for processing induced sputum for detection of Pneumocystis carinii. AU - Rocha,P, AU - Awe,R J, AU - Guy,E S, AU - Gaines,V, AU - Banez,E I, AU - Stager,C E, AU - Guntupalli,K K, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 52 EP - 7 JF - American journal of clinical pathology JO - Am J Clin Pathol VL - 105 IS - 1 N2 - The authors describe a method to process induced sputum specimens for detection of Pneumocystis carinii which is simple, rapid and inexpensive. Induced sputum and bronchoalveolar lavage (BAL) were obtained within a 24-hour period from 41 patients who were HIV-positive and had pulmonary symptoms suspicious for P carinii pneumonia. Induced sputum or BAL fluid was placed into Saccomanno's fixative, blended, and centrifuged. The sediment was stained for P carinii cysts by a modified method with Fungi-Fluor Solution A (Polysciences, Warington, PA) and the Genetic Systems Pneumocystis carinii Immunofluorescence Antibody (Genetic Systems, Seattle, WA). The Genetic Systems stain on the BAL specimen was positive in 35 patients and was the standard for comparison. With a single induced sputum, the Genetic Systems stain detected 31 (89%) positive patients, whereas the Fungi-Fluor stain detected 21 (60%). The sensitivity for detecting P carinii cysts in induced sputum was significantly greater (P < 0.05) for the Genetic Systems stain. SN - 0002-9173 UR - https://www.unboundmedicine.com/medline/citation/8561088/A_rapid_and_inexpensive_method_for_processing_induced_sputum_for_detection_of_Pneumocystis_carinii_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1093/ajcp/105.1.52 DB - PRIME DP - Unbound Medicine ER -