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Rooster testicular germ cells and epididymal sperm contain P450 aromatase.
Biol Reprod. 1995 Dec; 53(6):1259-64.BR

Abstract

We recently found that cytochrome P450 aromatase (P450arom) is present in germ cells of the mammalian testis and is capable of converting androgens to estrogens in the male reproductive tract. The objective of the present study was to determine whether testicular germ cells and epididymal sperm of an avian species are also capable of synthesizing estrogen. P450arom was localized in the rooster testis and epididymal region by immunocytochemistry, using an antiserum generated against purified human placental cytochrome P450arom. Immunostaining was present in pachytene spermatocytes, round spermatids, elongated spermatids, flagella of late spermatids, and sperm in the epididymal region. A positive reaction was also found in nonciliated cells of the epididymal region. However, the absence of mRNA for P450arom in the epididymal region indicated that the immunoreactive protein present in the epididymal region is not synthesized in this region. The immunoreactive P450arom found in epididymal sperm was shown to be active through use of a 3H2O assay. On the basis of these data, we conclude that rooster testicular germ cells and epididymal sperm are sites for the synthesis of estrogen, a potential regulator or modulator of germinal epithelium in the testis and the epithelium of the epididymal region of the avian species.

Authors+Show Affiliations

Department of Animal Sciences, University of Illinois, Urbana 61801, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8562679

Citation

Kwon, S, et al. "Rooster Testicular Germ Cells and Epididymal Sperm Contain P450 Aromatase." Biology of Reproduction, vol. 53, no. 6, 1995, pp. 1259-64.
Kwon S, Hess RA, Bunick D, et al. Rooster testicular germ cells and epididymal sperm contain P450 aromatase. Biol Reprod. 1995;53(6):1259-64.
Kwon, S., Hess, R. A., Bunick, D., Nitta, H., Janulis, L., Osawa, Y., & Bahr, J. M. (1995). Rooster testicular germ cells and epididymal sperm contain P450 aromatase. Biology of Reproduction, 53(6), 1259-64.
Kwon S, et al. Rooster Testicular Germ Cells and Epididymal Sperm Contain P450 Aromatase. Biol Reprod. 1995;53(6):1259-64. PubMed PMID: 8562679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rooster testicular germ cells and epididymal sperm contain P450 aromatase. AU - Kwon,S, AU - Hess,R A, AU - Bunick,D, AU - Nitta,H, AU - Janulis,L, AU - Osawa,Y, AU - Bahr,J M, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 1259 EP - 64 JF - Biology of reproduction JO - Biol Reprod VL - 53 IS - 6 N2 - We recently found that cytochrome P450 aromatase (P450arom) is present in germ cells of the mammalian testis and is capable of converting androgens to estrogens in the male reproductive tract. The objective of the present study was to determine whether testicular germ cells and epididymal sperm of an avian species are also capable of synthesizing estrogen. P450arom was localized in the rooster testis and epididymal region by immunocytochemistry, using an antiserum generated against purified human placental cytochrome P450arom. Immunostaining was present in pachytene spermatocytes, round spermatids, elongated spermatids, flagella of late spermatids, and sperm in the epididymal region. A positive reaction was also found in nonciliated cells of the epididymal region. However, the absence of mRNA for P450arom in the epididymal region indicated that the immunoreactive protein present in the epididymal region is not synthesized in this region. The immunoreactive P450arom found in epididymal sperm was shown to be active through use of a 3H2O assay. On the basis of these data, we conclude that rooster testicular germ cells and epididymal sperm are sites for the synthesis of estrogen, a potential regulator or modulator of germinal epithelium in the testis and the epithelium of the epididymal region of the avian species. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/8562679/Rooster_testicular_germ_cells_and_epididymal_sperm_contain_P450_aromatase_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod53.6.1259 DB - PRIME DP - Unbound Medicine ER -