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CCAAT/enhancer binding protein isoforms expression in the colon of neonatal mice.
Dev Dyn. 1995 Sep; 204(1):66-76.DD

Abstract

Development of murine proximal colon follows a complex pattern of morphological and functional differentiation. Molecular mechanisms and factors responsible for colon-specific gene expression remain to be established. In an attempt to identify some of these factors, we examined the expression of the alpha, beta, and delta isoforms of the CCAAT/enhancer binding protein (C/EBP) transcription factor gene family during murine colon development. Whereas C/EBP alpha mRNA levels are reduced during the third post-natal week, C/EBP alpha 42 and 30 kD proteins levels decrease between post-natal days 8 and 21. C/EBP beta mRNA levels increase between post-natal days 4 and 8 and remain constant subsequently, in contrast to a decrease in C/EBP beta protein levels between post-natal days 11 and 15. C/EBP delta mRNA levels increase gradually while C/EBP delta protein levels show variations during post-natal development. Changes in C/EBP DNA binding activity coincides with modifications in C/EBP isoforms expression. By indirect immunofluorescence, we show restriction of C/EBP alpha expression to differentiated surface epithelial cells during crypt formation. C/EBP alpha is predominantly nuclear with some cytoplasmic staining at all developmental stages. C/EBP beta and delta are both predominantly nuclear in crypt and differentiated surface epithelial cells, as well as in various cells of the lamina propria and muscular layers. Thus, specific C/EBP isoforms are differentially regulated during murine colon post-natal development. Differential C/EBP isoforms pattern of expression suggests a role for these transcription factors in colon-specific gene expression during development.

Authors+Show Affiliations

Département d'Anatomie et Biologie Cellulaire, Faculté de Médecine, Université de Sherbrooke, Québec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8563027

Citation

Blais, S, et al. "CCAAT/enhancer Binding Protein Isoforms Expression in the Colon of Neonatal Mice." Developmental Dynamics : an Official Publication of the American Association of Anatomists, vol. 204, no. 1, 1995, pp. 66-76.
Blais S, Boudreau F, Beaulieu JF, et al. CCAAT/enhancer binding protein isoforms expression in the colon of neonatal mice. Dev Dyn. 1995;204(1):66-76.
Blais, S., Boudreau, F., Beaulieu, J. F., & Asselin, C. (1995). CCAAT/enhancer binding protein isoforms expression in the colon of neonatal mice. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 204(1), 66-76.
Blais S, et al. CCAAT/enhancer Binding Protein Isoforms Expression in the Colon of Neonatal Mice. Dev Dyn. 1995;204(1):66-76. PubMed PMID: 8563027.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CCAAT/enhancer binding protein isoforms expression in the colon of neonatal mice. AU - Blais,S, AU - Boudreau,F, AU - Beaulieu,J F, AU - Asselin,C, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 66 EP - 76 JF - Developmental dynamics : an official publication of the American Association of Anatomists JO - Dev Dyn VL - 204 IS - 1 N2 - Development of murine proximal colon follows a complex pattern of morphological and functional differentiation. Molecular mechanisms and factors responsible for colon-specific gene expression remain to be established. In an attempt to identify some of these factors, we examined the expression of the alpha, beta, and delta isoforms of the CCAAT/enhancer binding protein (C/EBP) transcription factor gene family during murine colon development. Whereas C/EBP alpha mRNA levels are reduced during the third post-natal week, C/EBP alpha 42 and 30 kD proteins levels decrease between post-natal days 8 and 21. C/EBP beta mRNA levels increase between post-natal days 4 and 8 and remain constant subsequently, in contrast to a decrease in C/EBP beta protein levels between post-natal days 11 and 15. C/EBP delta mRNA levels increase gradually while C/EBP delta protein levels show variations during post-natal development. Changes in C/EBP DNA binding activity coincides with modifications in C/EBP isoforms expression. By indirect immunofluorescence, we show restriction of C/EBP alpha expression to differentiated surface epithelial cells during crypt formation. C/EBP alpha is predominantly nuclear with some cytoplasmic staining at all developmental stages. C/EBP beta and delta are both predominantly nuclear in crypt and differentiated surface epithelial cells, as well as in various cells of the lamina propria and muscular layers. Thus, specific C/EBP isoforms are differentially regulated during murine colon post-natal development. Differential C/EBP isoforms pattern of expression suggests a role for these transcription factors in colon-specific gene expression during development. SN - 1058-8388 UR - https://www.unboundmedicine.com/medline/citation/8563027/CCAAT/enhancer_binding_protein_isoforms_expression_in_the_colon_of_neonatal_mice_ DB - PRIME DP - Unbound Medicine ER -