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Relaxin-induced increased coronary flow through stimulation of nitric oxide production.
Br J Pharmacol. 1995 Sep; 116(1):1589-94.BJ

Abstract

1. Relaxin (RLX) is a multifunctional hormone which, besides its role in pregnancy and parturition, has also been shown to influence the cardiovascular system. In this study, we investigated the effect of RLX on coronary flow of rat and guinea-pig hearts, isolated and perfused in a Langendorff apparatus. RLX was either added to the perfusion fluid at a concentration of 5 x 10(-9) M for a 20-min perfusion, or given as a bolus into the aortic cannula at concentrations of 10(-9) M, 5 x 10(-8) M dissolved in 1 ml of perfusion fluid. 2. RLX, given either for a 20-min perfusion or as a bolus in the aortic cannula to guinea-pig and rat isolated hearts, increased the coronary flow and the amount of nitrite, a stable end-product of nitric oxide (NO) metabolism, that appeared in the perfusates in a concentration-dependent fashion. 3. The increase in coronary flow and in nitrite in the perfusates induced by RLX was significantly reduced by pretreatment with the nitric oxide synthase (NOS) inhibitor, NG-monomethyl-L-arginine (L-NMMA, 10(-4) M). 4. The effects of RLX on coronary flow and nitrite amounts in the perfusates were compared with those induced by the endothelium-dependent vasodilator agent, acetylcholine (ACh, 10(-8)-10(-7) M), and by the endothelium-independent vasodilator agent, sodium nitroprusside (SNP, 10(-7)-10(-6) M). The results obtained show that RLX is more effective than ACh and SNP in increasing coronary flow. 5 The results of this study show that RLX increases coronary flow through stimulation of NO production; hence this hormone should be regarded as a novel agent capable of improving myocardial perfusion.

Authors+Show Affiliations

Department of Human Anatomy and Histology, University of Florence, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8564223

Citation

Bani-Sacchi, T, et al. "Relaxin-induced Increased Coronary Flow Through Stimulation of Nitric Oxide Production." British Journal of Pharmacology, vol. 116, no. 1, 1995, pp. 1589-94.
Bani-Sacchi T, Bigazzi M, Bani D, et al. Relaxin-induced increased coronary flow through stimulation of nitric oxide production. Br J Pharmacol. 1995;116(1):1589-94.
Bani-Sacchi, T., Bigazzi, M., Bani, D., Mannaioni, P. F., & Masini, E. (1995). Relaxin-induced increased coronary flow through stimulation of nitric oxide production. British Journal of Pharmacology, 116(1), 1589-94.
Bani-Sacchi T, et al. Relaxin-induced Increased Coronary Flow Through Stimulation of Nitric Oxide Production. Br J Pharmacol. 1995;116(1):1589-94. PubMed PMID: 8564223.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relaxin-induced increased coronary flow through stimulation of nitric oxide production. AU - Bani-Sacchi,T, AU - Bigazzi,M, AU - Bani,D, AU - Mannaioni,P F, AU - Masini,E, PY - 1995/9/1/pubmed PY - 1995/9/1/medline PY - 1995/9/1/entrez SP - 1589 EP - 94 JF - British journal of pharmacology JO - Br. J. Pharmacol. VL - 116 IS - 1 N2 - 1. Relaxin (RLX) is a multifunctional hormone which, besides its role in pregnancy and parturition, has also been shown to influence the cardiovascular system. In this study, we investigated the effect of RLX on coronary flow of rat and guinea-pig hearts, isolated and perfused in a Langendorff apparatus. RLX was either added to the perfusion fluid at a concentration of 5 x 10(-9) M for a 20-min perfusion, or given as a bolus into the aortic cannula at concentrations of 10(-9) M, 5 x 10(-8) M dissolved in 1 ml of perfusion fluid. 2. RLX, given either for a 20-min perfusion or as a bolus in the aortic cannula to guinea-pig and rat isolated hearts, increased the coronary flow and the amount of nitrite, a stable end-product of nitric oxide (NO) metabolism, that appeared in the perfusates in a concentration-dependent fashion. 3. The increase in coronary flow and in nitrite in the perfusates induced by RLX was significantly reduced by pretreatment with the nitric oxide synthase (NOS) inhibitor, NG-monomethyl-L-arginine (L-NMMA, 10(-4) M). 4. The effects of RLX on coronary flow and nitrite amounts in the perfusates were compared with those induced by the endothelium-dependent vasodilator agent, acetylcholine (ACh, 10(-8)-10(-7) M), and by the endothelium-independent vasodilator agent, sodium nitroprusside (SNP, 10(-7)-10(-6) M). The results obtained show that RLX is more effective than ACh and SNP in increasing coronary flow. 5 The results of this study show that RLX increases coronary flow through stimulation of NO production; hence this hormone should be regarded as a novel agent capable of improving myocardial perfusion. SN - 0007-1188 UR - https://www.unboundmedicine.com/medline/citation/8564223/Relaxin_induced_increased_coronary_flow_through_stimulation_of_nitric_oxide_production_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-1188&date=1995&volume=116&issue=1&spage=1589 DB - PRIME DP - Unbound Medicine ER -