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[Quantitative urine microscopic examination using disposable counting chamber for diagnosis of urinary tract infection].
Rinsho Byori. 1995 Dec; 43(12):1273-8.RB

Abstract

Routine urinalysis is performed as a screening test for urinary tract infection (UTI) in out-patients or in-patients. We assessed the usefulness of microscopic examination of unspun and unstained urine using a disposable slide with counting chambers (Kova Slide 10 grid, Miles-Sankyo) for diagnosis of significant bacteriuria. 173 fresh urine samples were obtained from 173 subjects (89 male and 84 (female), including 117 inpatients, aged from 0 to 96 years. Urine samples were examined for bacteriuria by the standard culture method and counting chamber method. Significant bacteriuria was defined as 10(5)/ml or more of bacilli for midstream urine and urine collected by bags and 10(4)/ml or more for urine collected by catheterization and from indwelling catheters. Urine leukocytes were also counted on disposable slide. The rapid dipstick test (N-multistix-SG-10, Miles-Sankyo) of leukocyte esterase activity and nitrite were measured in the urine specimens read by a photometer (Clinitek-10, Miles-Sankyo). Significant bacteriuria was diagnosed by standard culture method in 67 urine samples. Close correlation was obtained between bacterial counts determined by the bacterial culture and counting chamber method (Spearman's correlation coefficient p < 0.001). Sensitivity and negative predictive value for significant bacteriuria were 94.0 and 95.1%, respectively, when bacteriuria or pyuria was present in the counting chamber. Dipstick test had a sensitivity and negative predictive value of 86.6 and 89.9%, respectively, when either leukocyte esterase activity of + or more, or nitrite of + was found. In out-patients, both sensitivity and negative predictive value were as high as 100% in counting chamber method. Thus, we can conclude that urine microscopy on disposable counting chambers is a very sensitive, simple, time-saving and lost-effective method for diagnosis of UTI.

Authors+Show Affiliations

Department of Clinical Laboratory, Fukui Medical School Hospital.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

jpn

PubMed ID

8569040

Citation

Hida, Y, et al. "[Quantitative Urine Microscopic Examination Using Disposable Counting Chamber for Diagnosis of Urinary Tract Infection]." Rinsho Byori. the Japanese Journal of Clinical Pathology, vol. 43, no. 12, 1995, pp. 1273-8.
Hida Y, Yamashita M, Gejyo F, et al. [Quantitative urine microscopic examination using disposable counting chamber for diagnosis of urinary tract infection]. Rinsho Byori. 1995;43(12):1273-8.
Hida, Y., Yamashita, M., Gejyo, F., Hiraoka, M., Hori, C., & Sudo, M. (1995). [Quantitative urine microscopic examination using disposable counting chamber for diagnosis of urinary tract infection]. Rinsho Byori. the Japanese Journal of Clinical Pathology, 43(12), 1273-8.
Hida Y, et al. [Quantitative Urine Microscopic Examination Using Disposable Counting Chamber for Diagnosis of Urinary Tract Infection]. Rinsho Byori. 1995;43(12):1273-8. PubMed PMID: 8569040.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Quantitative urine microscopic examination using disposable counting chamber for diagnosis of urinary tract infection]. AU - Hida,Y, AU - Yamashita,M, AU - Gejyo,F, AU - Hiraoka,M, AU - Hori,C, AU - Sudo,M, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 1273 EP - 8 JF - Rinsho byori. The Japanese journal of clinical pathology JO - Rinsho Byori VL - 43 IS - 12 N2 - Routine urinalysis is performed as a screening test for urinary tract infection (UTI) in out-patients or in-patients. We assessed the usefulness of microscopic examination of unspun and unstained urine using a disposable slide with counting chambers (Kova Slide 10 grid, Miles-Sankyo) for diagnosis of significant bacteriuria. 173 fresh urine samples were obtained from 173 subjects (89 male and 84 (female), including 117 inpatients, aged from 0 to 96 years. Urine samples were examined for bacteriuria by the standard culture method and counting chamber method. Significant bacteriuria was defined as 10(5)/ml or more of bacilli for midstream urine and urine collected by bags and 10(4)/ml or more for urine collected by catheterization and from indwelling catheters. Urine leukocytes were also counted on disposable slide. The rapid dipstick test (N-multistix-SG-10, Miles-Sankyo) of leukocyte esterase activity and nitrite were measured in the urine specimens read by a photometer (Clinitek-10, Miles-Sankyo). Significant bacteriuria was diagnosed by standard culture method in 67 urine samples. Close correlation was obtained between bacterial counts determined by the bacterial culture and counting chamber method (Spearman's correlation coefficient p < 0.001). Sensitivity and negative predictive value for significant bacteriuria were 94.0 and 95.1%, respectively, when bacteriuria or pyuria was present in the counting chamber. Dipstick test had a sensitivity and negative predictive value of 86.6 and 89.9%, respectively, when either leukocyte esterase activity of + or more, or nitrite of + was found. In out-patients, both sensitivity and negative predictive value were as high as 100% in counting chamber method. Thus, we can conclude that urine microscopy on disposable counting chambers is a very sensitive, simple, time-saving and lost-effective method for diagnosis of UTI. SN - 0047-1860 UR - https://www.unboundmedicine.com/medline/citation/8569040/[Quantitative_urine_microscopic_examination_using_disposable_counting_chamber_for_diagnosis_of_urinary_tract_infection]_ L2 - http://www.diseaseinfosearch.org/result/9683 DB - PRIME DP - Unbound Medicine ER -