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Enzyme-linked immunosorbent assay for detection of antibody to lymphocytic choriomeningitis virus in mouse sera, with recombinant nucleoprotein as antigen.
Lab Anim Sci. 1995 Oct; 45(5):493-6.LA

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibody to lymphocytic choriomeningitis virus (LCMV) in mouse sera. This assay is based on recombinant LCMV nucleoprotein generated in a baculovirus system. Sera from experimentally and naturally infected as well as noninfected mice were tested, and the results were compared with those obtained from an established immunofluorescence assay (IFA) that uses infected cells as antigen. An excellent correlation was found; the ELISA specificity and sensitivity were calculated to be 100 and 95% respectively. Unlike the IFA, this ELISA does not require the handling of infective virus. It eliminates the need to work with a zoonotic agent in the laboratory while allowing effective screening of laboratory mouse populations for LCMV antibody.

Authors+Show Affiliations

Institute of Laboratory Animal Science, University of Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8569145

Citation

Homberger, F R., et al. "Enzyme-linked Immunosorbent Assay for Detection of Antibody to Lymphocytic Choriomeningitis Virus in Mouse Sera, With Recombinant Nucleoprotein as Antigen." Laboratory Animal Science, vol. 45, no. 5, 1995, pp. 493-6.
Homberger FR, Romano TP, Seiler P, et al. Enzyme-linked immunosorbent assay for detection of antibody to lymphocytic choriomeningitis virus in mouse sera, with recombinant nucleoprotein as antigen. Lab Anim Sci. 1995;45(5):493-6.
Homberger, F. R., Romano, T. P., Seiler, P., Hansen, G. M., & Smith, A. L. (1995). Enzyme-linked immunosorbent assay for detection of antibody to lymphocytic choriomeningitis virus in mouse sera, with recombinant nucleoprotein as antigen. Laboratory Animal Science, 45(5), 493-6.
Homberger FR, et al. Enzyme-linked Immunosorbent Assay for Detection of Antibody to Lymphocytic Choriomeningitis Virus in Mouse Sera, With Recombinant Nucleoprotein as Antigen. Lab Anim Sci. 1995;45(5):493-6. PubMed PMID: 8569145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzyme-linked immunosorbent assay for detection of antibody to lymphocytic choriomeningitis virus in mouse sera, with recombinant nucleoprotein as antigen. AU - Homberger,F R, AU - Romano,T P, AU - Seiler,P, AU - Hansen,G M, AU - Smith,A L, PY - 1995/10/1/pubmed PY - 1995/10/1/medline PY - 1995/10/1/entrez SP - 493 EP - 6 JF - Laboratory animal science JO - Lab Anim Sci VL - 45 IS - 5 N2 - An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibody to lymphocytic choriomeningitis virus (LCMV) in mouse sera. This assay is based on recombinant LCMV nucleoprotein generated in a baculovirus system. Sera from experimentally and naturally infected as well as noninfected mice were tested, and the results were compared with those obtained from an established immunofluorescence assay (IFA) that uses infected cells as antigen. An excellent correlation was found; the ELISA specificity and sensitivity were calculated to be 100 and 95% respectively. Unlike the IFA, this ELISA does not require the handling of infective virus. It eliminates the need to work with a zoonotic agent in the laboratory while allowing effective screening of laboratory mouse populations for LCMV antibody. SN - 0023-6764 UR - https://www.unboundmedicine.com/medline/citation/8569145/Enzyme_linked_immunosorbent_assay_for_detection_of_antibody_to_lymphocytic_choriomeningitis_virus_in_mouse_sera_with_recombinant_nucleoprotein_as_antigen_ DB - PRIME DP - Unbound Medicine ER -