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Cross-reaction but no avidity change of the serum antibody response after influenza vaccination.
Vaccine 1995; 13(16):1522-8V

Abstract

Pre- and post-vaccination sera from 19 volunteers were analysed by the haemagglutination inhibition (HI) test, virus neutralization (VN) assay and avidity enzyme-linked immunosorbent assay (ELISA). The sera were tested against the three strains in a commercial inactivated influenza vaccine; A/Beijing/353/89(H3N2); A/Taiwan/1/86 (H1N1) and B/Yamagata/16/88. Additionally, a range of earlier strains and one newer isolate were assayed for HI- and VN-antibodies. Large variations in the pre-vaccination HI titres were observed for the viruses tested. However, 8-9 days after vaccination HI titres increased to above the assumed protective level (HI > or = 40) in most subjects. Although a limited number of patients were analysed at each sampling point, the time-profile we observed in this study is consistent with data we have obtained in earlier trials (Cox, R.J. et al., Vaccine 1994, 12,993-999). The VN titres, on the other hand, were low against all influenza strains before and up to 6 days, but increased rapidly 8-9 days after vaccination. A recent H3N2 isolate, A/Beijing/32/92 (H3N2), which had drifted further away from the vaccine strain, reacted to low titres or were negative in both the HI and VN assays. No change in the serum avidity to the influenza surface antigens was detected after vaccination, whereas sera from subjects naturally infected with influenza showed an increase in avidity to the infecting virus strain. The increase in serum avidity observed in the infected subjects is probably due to the increased and prolonged antigenic stimulus provided by the replicating virus.

Authors+Show Affiliations

Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8578836

Citation

Brokstad, K A., et al. "Cross-reaction but No Avidity Change of the Serum Antibody Response After Influenza Vaccination." Vaccine, vol. 13, no. 16, 1995, pp. 1522-8.
Brokstad KA, Cox RJ, Major D, et al. Cross-reaction but no avidity change of the serum antibody response after influenza vaccination. Vaccine. 1995;13(16):1522-8.
Brokstad, K. A., Cox, R. J., Major, D., Wood, J. M., & Haaheim, L. R. (1995). Cross-reaction but no avidity change of the serum antibody response after influenza vaccination. Vaccine, 13(16), pp. 1522-8.
Brokstad KA, et al. Cross-reaction but No Avidity Change of the Serum Antibody Response After Influenza Vaccination. Vaccine. 1995;13(16):1522-8. PubMed PMID: 8578836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-reaction but no avidity change of the serum antibody response after influenza vaccination. AU - Brokstad,K A, AU - Cox,R J, AU - Major,D, AU - Wood,J M, AU - Haaheim,L R, PY - 1995/11/1/pubmed PY - 1995/11/1/medline PY - 1995/11/1/entrez SP - 1522 EP - 8 JF - Vaccine JO - Vaccine VL - 13 IS - 16 N2 - Pre- and post-vaccination sera from 19 volunteers were analysed by the haemagglutination inhibition (HI) test, virus neutralization (VN) assay and avidity enzyme-linked immunosorbent assay (ELISA). The sera were tested against the three strains in a commercial inactivated influenza vaccine; A/Beijing/353/89(H3N2); A/Taiwan/1/86 (H1N1) and B/Yamagata/16/88. Additionally, a range of earlier strains and one newer isolate were assayed for HI- and VN-antibodies. Large variations in the pre-vaccination HI titres were observed for the viruses tested. However, 8-9 days after vaccination HI titres increased to above the assumed protective level (HI > or = 40) in most subjects. Although a limited number of patients were analysed at each sampling point, the time-profile we observed in this study is consistent with data we have obtained in earlier trials (Cox, R.J. et al., Vaccine 1994, 12,993-999). The VN titres, on the other hand, were low against all influenza strains before and up to 6 days, but increased rapidly 8-9 days after vaccination. A recent H3N2 isolate, A/Beijing/32/92 (H3N2), which had drifted further away from the vaccine strain, reacted to low titres or were negative in both the HI and VN assays. No change in the serum avidity to the influenza surface antigens was detected after vaccination, whereas sera from subjects naturally infected with influenza showed an increase in avidity to the infecting virus strain. The increase in serum avidity observed in the infected subjects is probably due to the increased and prolonged antigenic stimulus provided by the replicating virus. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/8578836/Cross_reaction_but_no_avidity_change_of_the_serum_antibody_response_after_influenza_vaccination_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0264410X9500095I DB - PRIME DP - Unbound Medicine ER -