Tags

Type your tag names separated by a space and hit enter

Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation.
Free Radic Biol Med 1995; 19(6):725-33FR

Abstract

Antioxidants such as vitamin E protect unsaturated fatty acids of LDL against oxidation. In the ex vivo model used, LDL was exposed to Cu2+ ions, a potent prooxidant capable of initiating the oxidation of LDL. The lag time, indicating the delay of conjugated diene formation in LDL due to antioxidant protection, was measured in 54 cystic fibrosis (CF) patients with plasma alpha-tocopherol levels below (Group A, n = 30) or above (Group B, n = 24) 15.9 mumol/L (mean - 2 SD of Swiss population). Patients were reevaluated after 2 months on 400 IU/d of oral RRR-alpha-tocopherol. In group A, alpha-tocopherol concentrations in LDL increased significantly from 3.2 +/- 1.6 mol/mol LDL to 8.2 +/- 2.8 mol/mol (P < 0.001) and lag times increased from 79 +/- 33 min to 126 +/- 48 min (P < 0.001), whereas in the vitamin E sufficient group B no further increase neither in LDL alpha-tocopherol concentrations or in lag times was observed. LDL oleic acid concentrations were higher, and linoleic acid concentrations were lower in patients than in controls. After efficient vitamin E supplementation, lag times were positively related to LDL alpha-tocopherol (P < 0.01) and negatively to LDL linoleic and arachidonic acid content (P < 0.001). The maximum rate of oxidation correlated positively with linoleic and arachidonic acid concentrations, as did the maximum conjugated diene absorbance. These results indicate that LDL resistance to oxidation is impaired in vitamin E deficient CF patients but can be normalized within 2 months when alpha-tocopherol is given in sufficient amounts. Linoleic and arachidonic acid content exhibit a major influence on the LDL resistance to oxidation.

Authors+Show Affiliations

Department of Pediatrics, University of Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8582644

Citation

Winklhofer-Roob, B M., et al. "Impaired Resistance to Oxidation of Low Density Lipoprotein in Cystic Fibrosis: Improvement During Vitamin E Supplementation." Free Radical Biology & Medicine, vol. 19, no. 6, 1995, pp. 725-33.
Winklhofer-Roob BM, Ziouzenkova O, Puhl H, et al. Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. Free Radic Biol Med. 1995;19(6):725-33.
Winklhofer-Roob, B. M., Ziouzenkova, O., Puhl, H., Ellemunter, H., Greiner, P., Müller, G., ... Shmerling, D. H. (1995). Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. Free Radical Biology & Medicine, 19(6), pp. 725-33.
Winklhofer-Roob BM, et al. Impaired Resistance to Oxidation of Low Density Lipoprotein in Cystic Fibrosis: Improvement During Vitamin E Supplementation. Free Radic Biol Med. 1995;19(6):725-33. PubMed PMID: 8582644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. AU - Winklhofer-Roob,B M, AU - Ziouzenkova,O, AU - Puhl,H, AU - Ellemunter,H, AU - Greiner,P, AU - Müller,G, AU - van't Hof,M A, AU - Esterbauer,H, AU - Shmerling,D H, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 725 EP - 33 JF - Free radical biology & medicine JO - Free Radic. Biol. Med. VL - 19 IS - 6 N2 - Antioxidants such as vitamin E protect unsaturated fatty acids of LDL against oxidation. In the ex vivo model used, LDL was exposed to Cu2+ ions, a potent prooxidant capable of initiating the oxidation of LDL. The lag time, indicating the delay of conjugated diene formation in LDL due to antioxidant protection, was measured in 54 cystic fibrosis (CF) patients with plasma alpha-tocopherol levels below (Group A, n = 30) or above (Group B, n = 24) 15.9 mumol/L (mean - 2 SD of Swiss population). Patients were reevaluated after 2 months on 400 IU/d of oral RRR-alpha-tocopherol. In group A, alpha-tocopherol concentrations in LDL increased significantly from 3.2 +/- 1.6 mol/mol LDL to 8.2 +/- 2.8 mol/mol (P < 0.001) and lag times increased from 79 +/- 33 min to 126 +/- 48 min (P < 0.001), whereas in the vitamin E sufficient group B no further increase neither in LDL alpha-tocopherol concentrations or in lag times was observed. LDL oleic acid concentrations were higher, and linoleic acid concentrations were lower in patients than in controls. After efficient vitamin E supplementation, lag times were positively related to LDL alpha-tocopherol (P < 0.01) and negatively to LDL linoleic and arachidonic acid content (P < 0.001). The maximum rate of oxidation correlated positively with linoleic and arachidonic acid concentrations, as did the maximum conjugated diene absorbance. These results indicate that LDL resistance to oxidation is impaired in vitamin E deficient CF patients but can be normalized within 2 months when alpha-tocopherol is given in sufficient amounts. Linoleic and arachidonic acid content exhibit a major influence on the LDL resistance to oxidation. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/8582644/Impaired_resistance_to_oxidation_of_low_density_lipoprotein_in_cystic_fibrosis:_improvement_during_vitamin_E_supplementation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0891584995000634 DB - PRIME DP - Unbound Medicine ER -