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Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation.

Abstract

Antioxidants such as vitamin E protect unsaturated fatty acids of LDL against oxidation. In the ex vivo model used, LDL was exposed to Cu2+ ions, a potent prooxidant capable of initiating the oxidation of LDL. The lag time, indicating the delay of conjugated diene formation in LDL due to antioxidant protection, was measured in 54 cystic fibrosis (CF) patients with plasma alpha-tocopherol levels below (Group A, n = 30) or above (Group B, n = 24) 15.9 mumol/L (mean - 2 SD of Swiss population). Patients were reevaluated after 2 months on 400 IU/d of oral RRR-alpha-tocopherol. In group A, alpha-tocopherol concentrations in LDL increased significantly from 3.2 +/- 1.6 mol/mol LDL to 8.2 +/- 2.8 mol/mol (P < 0.001) and lag times increased from 79 +/- 33 min to 126 +/- 48 min (P < 0.001), whereas in the vitamin E sufficient group B no further increase neither in LDL alpha-tocopherol concentrations or in lag times was observed. LDL oleic acid concentrations were higher, and linoleic acid concentrations were lower in patients than in controls. After efficient vitamin E supplementation, lag times were positively related to LDL alpha-tocopherol (P < 0.01) and negatively to LDL linoleic and arachidonic acid content (P < 0.001). The maximum rate of oxidation correlated positively with linoleic and arachidonic acid concentrations, as did the maximum conjugated diene absorbance. These results indicate that LDL resistance to oxidation is impaired in vitamin E deficient CF patients but can be normalized within 2 months when alpha-tocopherol is given in sufficient amounts. Linoleic and arachidonic acid content exhibit a major influence on the LDL resistance to oxidation.

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  • Authors+Show Affiliations

    ,

    Department of Pediatrics, University of Zurich, Switzerland.

    , , , , , , ,

    Source

    Free radical biology & medicine 19:6 1995 Dec pg 725-33

    MeSH

    Adult
    Antioxidants
    Copper
    Cystic Fibrosis
    Fatty Acids
    Humans
    Lipid Peroxidation
    Lipoproteins, LDL
    Oxidation-Reduction
    Vitamin E
    Vitamin E Deficiency

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    8582644

    Citation

    Winklhofer-Roob, B M., et al. "Impaired Resistance to Oxidation of Low Density Lipoprotein in Cystic Fibrosis: Improvement During Vitamin E Supplementation." Free Radical Biology & Medicine, vol. 19, no. 6, 1995, pp. 725-33.
    Winklhofer-Roob BM, Ziouzenkova O, Puhl H, et al. Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. Free Radic Biol Med. 1995;19(6):725-33.
    Winklhofer-Roob, B. M., Ziouzenkova, O., Puhl, H., Ellemunter, H., Greiner, P., Müller, G., ... Shmerling, D. H. (1995). Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. Free Radical Biology & Medicine, 19(6), pp. 725-33.
    Winklhofer-Roob BM, et al. Impaired Resistance to Oxidation of Low Density Lipoprotein in Cystic Fibrosis: Improvement During Vitamin E Supplementation. Free Radic Biol Med. 1995;19(6):725-33. PubMed PMID: 8582644.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Impaired resistance to oxidation of low density lipoprotein in cystic fibrosis: improvement during vitamin E supplementation. AU - Winklhofer-Roob,B M, AU - Ziouzenkova,O, AU - Puhl,H, AU - Ellemunter,H, AU - Greiner,P, AU - Müller,G, AU - van't Hof,M A, AU - Esterbauer,H, AU - Shmerling,D H, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 725 EP - 33 JF - Free radical biology & medicine JO - Free Radic. Biol. Med. VL - 19 IS - 6 N2 - Antioxidants such as vitamin E protect unsaturated fatty acids of LDL against oxidation. In the ex vivo model used, LDL was exposed to Cu2+ ions, a potent prooxidant capable of initiating the oxidation of LDL. The lag time, indicating the delay of conjugated diene formation in LDL due to antioxidant protection, was measured in 54 cystic fibrosis (CF) patients with plasma alpha-tocopherol levels below (Group A, n = 30) or above (Group B, n = 24) 15.9 mumol/L (mean - 2 SD of Swiss population). Patients were reevaluated after 2 months on 400 IU/d of oral RRR-alpha-tocopherol. In group A, alpha-tocopherol concentrations in LDL increased significantly from 3.2 +/- 1.6 mol/mol LDL to 8.2 +/- 2.8 mol/mol (P < 0.001) and lag times increased from 79 +/- 33 min to 126 +/- 48 min (P < 0.001), whereas in the vitamin E sufficient group B no further increase neither in LDL alpha-tocopherol concentrations or in lag times was observed. LDL oleic acid concentrations were higher, and linoleic acid concentrations were lower in patients than in controls. After efficient vitamin E supplementation, lag times were positively related to LDL alpha-tocopherol (P < 0.01) and negatively to LDL linoleic and arachidonic acid content (P < 0.001). The maximum rate of oxidation correlated positively with linoleic and arachidonic acid concentrations, as did the maximum conjugated diene absorbance. These results indicate that LDL resistance to oxidation is impaired in vitamin E deficient CF patients but can be normalized within 2 months when alpha-tocopherol is given in sufficient amounts. Linoleic and arachidonic acid content exhibit a major influence on the LDL resistance to oxidation. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/8582644/Impaired_resistance_to_oxidation_of_low_density_lipoprotein_in_cystic_fibrosis:_improvement_during_vitamin_E_supplementation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0891584995000634 DB - PRIME DP - Unbound Medicine ER -