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RRR-alpha-tocopheryl succinate inhibits DNA synthesis and enhances the production and secretion of biologically active transforming growth factor-beta by avian retrovirus-transformed lymphoid cells.
Nutr Cancer. 1995; 24(2):171-85.NC

Abstract

The RRR-alpha-tocopheryl succinate form of vitamin E, referred to as vitamin E succinate (VES), inhibits the proliferation of avian reticuloendotheliosis virus-transformed RECC-UTC4-1 (C4-1) lymphoblastoid cells in a dose-dependent manner in vitro. Analyses of conditioned medium (CM) from VES growth-inhibited cells revealed a potent antiproliferative activity. Characterization of the antiproliferative activity as transforming growth factor-beta (TGF-beta) was established by 1) growth inhibition of TGF-beta-responsive Mv1Lu mink lung and murine CTLL-2 cell lines, 2) a combination of physical characteristics including heat stability, acid stability, and Bio-Gel P-60 column chromatography elution profile, 3) neutralization of the antiproliferative activity by antibodies specific for TGF-beta, and 4) immunoprecipitation of metabolically labeled TGF-beta in CM from VES-treated C4-1 cells by use of TGF-beta-specific antibodies. Northern blot analyses of total cellular RNA revealed that VES does not alter the levels of constitutively expressed TGF-beta isoform-specific mRNAs; namely, VES does not alter the levels of the 3.9- and 4.1-kb TGF-beta 2 mRNAs, the 3.0-kb TGF-beta 3 mRNA, or the 2.5-, 2.7-, and 1.7-kb TGF-beta 4 mRNAs. The data show that VES inhibits C4-1 cell proliferation and induces the cells to produce and secrete active forms of TGF-beta, suggesting that one mechanism whereby VES inhibits C4-1 cell proliferation may be via the TGF-beta pathway for cellular growth control.

Authors+Show Affiliations

Genetics Institute, University of Texas at Austin 78712-1097, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8584453

Citation

Simmons-Menchaca, M, et al. "RRR-alpha-tocopheryl Succinate Inhibits DNA Synthesis and Enhances the Production and Secretion of Biologically Active Transforming Growth Factor-beta By Avian Retrovirus-transformed Lymphoid Cells." Nutrition and Cancer, vol. 24, no. 2, 1995, pp. 171-85.
Simmons-Menchaca M, Qian M, Yu W, et al. RRR-alpha-tocopheryl succinate inhibits DNA synthesis and enhances the production and secretion of biologically active transforming growth factor-beta by avian retrovirus-transformed lymphoid cells. Nutr Cancer. 1995;24(2):171-85.
Simmons-Menchaca, M., Qian, M., Yu, W., Sanders, B. G., & Kline, K. (1995). RRR-alpha-tocopheryl succinate inhibits DNA synthesis and enhances the production and secretion of biologically active transforming growth factor-beta by avian retrovirus-transformed lymphoid cells. Nutrition and Cancer, 24(2), 171-85.
Simmons-Menchaca M, et al. RRR-alpha-tocopheryl Succinate Inhibits DNA Synthesis and Enhances the Production and Secretion of Biologically Active Transforming Growth Factor-beta By Avian Retrovirus-transformed Lymphoid Cells. Nutr Cancer. 1995;24(2):171-85. PubMed PMID: 8584453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RRR-alpha-tocopheryl succinate inhibits DNA synthesis and enhances the production and secretion of biologically active transforming growth factor-beta by avian retrovirus-transformed lymphoid cells. AU - Simmons-Menchaca,M, AU - Qian,M, AU - Yu,W, AU - Sanders,B G, AU - Kline,K, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 171 EP - 85 JF - Nutrition and cancer JO - Nutr Cancer VL - 24 IS - 2 N2 - The RRR-alpha-tocopheryl succinate form of vitamin E, referred to as vitamin E succinate (VES), inhibits the proliferation of avian reticuloendotheliosis virus-transformed RECC-UTC4-1 (C4-1) lymphoblastoid cells in a dose-dependent manner in vitro. Analyses of conditioned medium (CM) from VES growth-inhibited cells revealed a potent antiproliferative activity. Characterization of the antiproliferative activity as transforming growth factor-beta (TGF-beta) was established by 1) growth inhibition of TGF-beta-responsive Mv1Lu mink lung and murine CTLL-2 cell lines, 2) a combination of physical characteristics including heat stability, acid stability, and Bio-Gel P-60 column chromatography elution profile, 3) neutralization of the antiproliferative activity by antibodies specific for TGF-beta, and 4) immunoprecipitation of metabolically labeled TGF-beta in CM from VES-treated C4-1 cells by use of TGF-beta-specific antibodies. Northern blot analyses of total cellular RNA revealed that VES does not alter the levels of constitutively expressed TGF-beta isoform-specific mRNAs; namely, VES does not alter the levels of the 3.9- and 4.1-kb TGF-beta 2 mRNAs, the 3.0-kb TGF-beta 3 mRNA, or the 2.5-, 2.7-, and 1.7-kb TGF-beta 4 mRNAs. The data show that VES inhibits C4-1 cell proliferation and induces the cells to produce and secrete active forms of TGF-beta, suggesting that one mechanism whereby VES inhibits C4-1 cell proliferation may be via the TGF-beta pathway for cellular growth control. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/8584453/RRR_alpha_tocopheryl_succinate_inhibits_DNA_synthesis_and_enhances_the_production_and_secretion_of_biologically_active_transforming_growth_factor_beta_by_avian_retrovirus_transformed_lymphoid_cells_ L2 - https://www.tandfonline.com/doi/full/10.1080/01635589509514405 DB - PRIME DP - Unbound Medicine ER -