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Evaluation of different fixation-permeabilization methods for simultaneous detection of surface, cytoplasmic markers and DNA analysis by flow cytometry in some human hematopoietic cell lines.
Neoplasma. 1995; 42(6):337-46.N

Abstract

Different fixation/permeabilization methods were investigated for their convenience for simultaneous detection of membrane marker/DNA staining or cytoplasmic marker/DNA by flow cytometry. Nine different methods were employed. The expression of membrane marker CD20 and cytoplasmic marker CD22 on BJAB and DAUDI cells and cytoplasmic CD3 on MOLT4 cells were measured. Optimal methods were those that combine paraformaldehyde and saponin (for membrane CD20/DNA staining and cytoplasmic CD3/DNA staining) or buffered formaldehyde-acetone (for membrane CD20/DNA staining and for cytoplasmic CD22/DNA staining). Special interest was focused on proliferation marker CD71 and nuclear antigen Ki67. We investigated CD71 in MOLT4 cells and Ki67 in MOLT4, DAUDI and BJAB cells. Both of these markers are closely associated with proliferation rate. The optimal method for detection of Ki67/DNA staining combines paraformaldehyde with Tween 20.

Authors+Show Affiliations

Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovakia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8592577

Citation

Glasová, M, et al. "Evaluation of Different Fixation-permeabilization Methods for Simultaneous Detection of Surface, Cytoplasmic Markers and DNA Analysis By Flow Cytometry in some Human Hematopoietic Cell Lines." Neoplasma, vol. 42, no. 6, 1995, pp. 337-46.
Glasová M, Koníková E, Kusenda J, et al. Evaluation of different fixation-permeabilization methods for simultaneous detection of surface, cytoplasmic markers and DNA analysis by flow cytometry in some human hematopoietic cell lines. Neoplasma. 1995;42(6):337-46.
Glasová, M., Koníková, E., Kusenda, J., & Babusíková, O. (1995). Evaluation of different fixation-permeabilization methods for simultaneous detection of surface, cytoplasmic markers and DNA analysis by flow cytometry in some human hematopoietic cell lines. Neoplasma, 42(6), 337-46.
Glasová M, et al. Evaluation of Different Fixation-permeabilization Methods for Simultaneous Detection of Surface, Cytoplasmic Markers and DNA Analysis By Flow Cytometry in some Human Hematopoietic Cell Lines. Neoplasma. 1995;42(6):337-46. PubMed PMID: 8592577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of different fixation-permeabilization methods for simultaneous detection of surface, cytoplasmic markers and DNA analysis by flow cytometry in some human hematopoietic cell lines. AU - Glasová,M, AU - Koníková,E, AU - Kusenda,J, AU - Babusíková,O, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 337 EP - 46 JF - Neoplasma JO - Neoplasma VL - 42 IS - 6 N2 - Different fixation/permeabilization methods were investigated for their convenience for simultaneous detection of membrane marker/DNA staining or cytoplasmic marker/DNA by flow cytometry. Nine different methods were employed. The expression of membrane marker CD20 and cytoplasmic marker CD22 on BJAB and DAUDI cells and cytoplasmic CD3 on MOLT4 cells were measured. Optimal methods were those that combine paraformaldehyde and saponin (for membrane CD20/DNA staining and cytoplasmic CD3/DNA staining) or buffered formaldehyde-acetone (for membrane CD20/DNA staining and for cytoplasmic CD22/DNA staining). Special interest was focused on proliferation marker CD71 and nuclear antigen Ki67. We investigated CD71 in MOLT4 cells and Ki67 in MOLT4, DAUDI and BJAB cells. Both of these markers are closely associated with proliferation rate. The optimal method for detection of Ki67/DNA staining combines paraformaldehyde with Tween 20. SN - 0028-2685 UR - https://www.unboundmedicine.com/medline/citation/8592577/Evaluation_of_different_fixation_permeabilization_methods_for_simultaneous_detection_of_surface_cytoplasmic_markers_and_DNA_analysis_by_flow_cytometry_in_some_human_hematopoietic_cell_lines_ L2 - https://medlineplus.gov/leukemia.html DB - PRIME DP - Unbound Medicine ER -