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Dissection of the Yersinia enterocolitica virulence plasmid pYVO8 into an operating unit and virulence gene modules.
FEMS Microbiol Lett 1995; 134(1):69-73FM

Abstract

The structural genes encoding the Yop proteins of Yersinia enterocolitica are scattered around on the virulence plasmid (pYV). The genes which are required for transactivation, secretion and translocation of the Yopos are encoded in one cluster known as the lcr-region of pYV. After the introduction of an additional SalI restriction site into pYV of Y. enterocolitica serotype O8, we were able to clone and isolate the whole lcr-region on the mobilizable low copy vector pSUP102. Analysis of this construct in a plasmidless WA-strain showed that all Yops being encoded inside the lcr-region (YopN, YopB, YopD and the V-antigen) were secreted into the culture supernatant. Moreover, this lcr-fragment was able to promote secretion of other Yops encoded by a second recombinant plasmid. Thus the translocation and function of single Yops can be studied.

Authors+Show Affiliations

Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8593958

Citation

Roggenkamp, A, et al. "Dissection of the Yersinia Enterocolitica Virulence Plasmid pYVO8 Into an Operating Unit and Virulence Gene Modules." FEMS Microbiology Letters, vol. 134, no. 1, 1995, pp. 69-73.
Roggenkamp A, Schubert S, Jacobi CA, et al. Dissection of the Yersinia enterocolitica virulence plasmid pYVO8 into an operating unit and virulence gene modules. FEMS Microbiol Lett. 1995;134(1):69-73.
Roggenkamp, A., Schubert, S., Jacobi, C. A., & Heesemann, J. (1995). Dissection of the Yersinia enterocolitica virulence plasmid pYVO8 into an operating unit and virulence gene modules. FEMS Microbiology Letters, 134(1), pp. 69-73.
Roggenkamp A, et al. Dissection of the Yersinia Enterocolitica Virulence Plasmid pYVO8 Into an Operating Unit and Virulence Gene Modules. FEMS Microbiol Lett. 1995 Dec 1;134(1):69-73. PubMed PMID: 8593958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissection of the Yersinia enterocolitica virulence plasmid pYVO8 into an operating unit and virulence gene modules. AU - Roggenkamp,A, AU - Schubert,S, AU - Jacobi,C A, AU - Heesemann,J, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 69 EP - 73 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 134 IS - 1 N2 - The structural genes encoding the Yop proteins of Yersinia enterocolitica are scattered around on the virulence plasmid (pYV). The genes which are required for transactivation, secretion and translocation of the Yopos are encoded in one cluster known as the lcr-region of pYV. After the introduction of an additional SalI restriction site into pYV of Y. enterocolitica serotype O8, we were able to clone and isolate the whole lcr-region on the mobilizable low copy vector pSUP102. Analysis of this construct in a plasmidless WA-strain showed that all Yops being encoded inside the lcr-region (YopN, YopB, YopD and the V-antigen) were secreted into the culture supernatant. Moreover, this lcr-fragment was able to promote secretion of other Yops encoded by a second recombinant plasmid. Thus the translocation and function of single Yops can be studied. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/8593958/Dissection_of_the_Yersinia_enterocolitica_virulence_plasmid_pYVO8_into_an_operating_unit_and_virulence_gene_modules_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.1995.tb07916.x DB - PRIME DP - Unbound Medicine ER -