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Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography.
Clin Chem. 1996 Feb; 42(2):292-7.CC

Abstract

We developed a gas-chromatographic procedure for the simultaneous determination of ethylene glycol (EG) and its major toxic metabolite, glycolic acid (GA), suitable for clinical use in instances of EG intoxication. After serum protein precipitation with acetonitrile (containing internal standard), the supernate is treated with 2,2-dimethoxypropane (containing dimethylformamide) to remove water, and the volume is then reduced by evaporation to <100 microL of dimethylformamide (but not to dryness). After trimethylsilyl derivatization, the resulting derivatives are analyzed by capillary column gas chromatography. Only 100 microL of serum is required and the entire determination, including calibrators and controls, takes <2 h. The method gives a linear response to at least 10 g/L EG and 5 g/L GA and has a limit of detection <10 mg/L. Intraassay CV is < or = 2.8% for EG (100 and 1000 mg/L) and GA (100 and 500 mg/L); between-day CV is < or = 6.5%. The absolute recovery from serum was 91% for EG and 77-82% for GA (200 and 2000 mg/L each). Relative to calibrators prepared bovine serum albumin (70 g/L), the recovery was 99-104% for EG (100 - 5000 mg/L) and 95-105% for GA (50 - 2500 mg/L). No clinically important interference was detected for >60 exogenous or endogenous compounds and drugs.

Authors+Show Affiliations

Department of Pathology and Laboratory Medicine, University of Kentucky Medical Center, Lexington, 40536-0084, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8595726

Citation

Yao, H H., and W H. Porter. "Simultaneous Determination of Ethylene Glycol and Its Major Toxic Metabolite, Glycolic Acid, in Serum By Gas Chromatography." Clinical Chemistry, vol. 42, no. 2, 1996, pp. 292-7.
Yao HH, Porter WH. Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography. Clin Chem. 1996;42(2):292-7.
Yao, H. H., & Porter, W. H. (1996). Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography. Clinical Chemistry, 42(2), 292-7.
Yao HH, Porter WH. Simultaneous Determination of Ethylene Glycol and Its Major Toxic Metabolite, Glycolic Acid, in Serum By Gas Chromatography. Clin Chem. 1996;42(2):292-7. PubMed PMID: 8595726.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of ethylene glycol and its major toxic metabolite, glycolic acid, in serum by gas chromatography. AU - Yao,H H, AU - Porter,W H, PY - 1996/2/1/pubmed PY - 1996/2/1/medline PY - 1996/2/1/entrez SP - 292 EP - 7 JF - Clinical chemistry JO - Clin Chem VL - 42 IS - 2 N2 - We developed a gas-chromatographic procedure for the simultaneous determination of ethylene glycol (EG) and its major toxic metabolite, glycolic acid (GA), suitable for clinical use in instances of EG intoxication. After serum protein precipitation with acetonitrile (containing internal standard), the supernate is treated with 2,2-dimethoxypropane (containing dimethylformamide) to remove water, and the volume is then reduced by evaporation to <100 microL of dimethylformamide (but not to dryness). After trimethylsilyl derivatization, the resulting derivatives are analyzed by capillary column gas chromatography. Only 100 microL of serum is required and the entire determination, including calibrators and controls, takes <2 h. The method gives a linear response to at least 10 g/L EG and 5 g/L GA and has a limit of detection <10 mg/L. Intraassay CV is < or = 2.8% for EG (100 and 1000 mg/L) and GA (100 and 500 mg/L); between-day CV is < or = 6.5%. The absolute recovery from serum was 91% for EG and 77-82% for GA (200 and 2000 mg/L each). Relative to calibrators prepared bovine serum albumin (70 g/L), the recovery was 99-104% for EG (100 - 5000 mg/L) and 95-105% for GA (50 - 2500 mg/L). No clinically important interference was detected for >60 exogenous or endogenous compounds and drugs. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/8595726/Simultaneous_determination_of_ethylene_glycol_and_its_major_toxic_metabolite_glycolic_acid_in_serum_by_gas_chromatography_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&amp;PAGE=linkout&amp;SEARCH=8595726.ui DB - PRIME DP - Unbound Medicine ER -