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Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns.
Science. 1996 Mar 22; 271(5256):1716-8.Sci

Abstract

A conserved sequence element in a minor class of eukaryotic pre-messenger RNA (pre-mRNA) introns was previously proposed to base pair with a complementary sequence in the U12 small nuclear RNA (snRNA) in a manner analogous to the pairing of US snRNA with the branch site sequence of the major class of introns. Here, mutations generated in this conserved sequence element block the splicing of a member of this minor intron class in vivo. The block was relieved by coexpression of a U12 snRNA containing compensatory mutations that restore the proposed base pairing interaction. These results show that this minor class of pre-mRNA introns is a distinct class existing alongside the major class of introns in animal genomes, and these results also establish an in vivo function for U12 snRNA.

Authors+Show Affiliations

Department of Molecular Biology, Cleveland Clinic Foundation, OH 44195, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8596930

Citation

Hall, S L., and R A. Padgett. "Requirement of U12 snRNA for in Vivo Splicing of a Minor Class of Eukaryotic Nuclear pre-mRNA Introns." Science (New York, N.Y.), vol. 271, no. 5256, 1996, pp. 1716-8.
Hall SL, Padgett RA. Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science. 1996;271(5256):1716-8.
Hall, S. L., & Padgett, R. A. (1996). Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. Science (New York, N.Y.), 271(5256), 1716-8.
Hall SL, Padgett RA. Requirement of U12 snRNA for in Vivo Splicing of a Minor Class of Eukaryotic Nuclear pre-mRNA Introns. Science. 1996 Mar 22;271(5256):1716-8. PubMed PMID: 8596930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns. AU - Hall,S L, AU - Padgett,R A, PY - 1996/3/22/pubmed PY - 1996/3/22/medline PY - 1996/3/22/entrez SP - 1716 EP - 8 JF - Science (New York, N.Y.) JO - Science VL - 271 IS - 5256 N2 - A conserved sequence element in a minor class of eukaryotic pre-messenger RNA (pre-mRNA) introns was previously proposed to base pair with a complementary sequence in the U12 small nuclear RNA (snRNA) in a manner analogous to the pairing of US snRNA with the branch site sequence of the major class of introns. Here, mutations generated in this conserved sequence element block the splicing of a member of this minor intron class in vivo. The block was relieved by coexpression of a U12 snRNA containing compensatory mutations that restore the proposed base pairing interaction. These results show that this minor class of pre-mRNA introns is a distinct class existing alongside the major class of introns in animal genomes, and these results also establish an in vivo function for U12 snRNA. SN - 0036-8075 UR - https://www.unboundmedicine.com/medline/citation/8596930/Requirement_of_U12_snRNA_for_in_vivo_splicing_of_a_minor_class_of_eukaryotic_nuclear_pre_mRNA_introns_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=8596930 DB - PRIME DP - Unbound Medicine ER -