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Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography.
Anal Biochem. 1995 Nov 20; 232(1):114-21.AB

Abstract

A series of disaccharides derived from chondroitin sulfate and heparin/heparan sulfate were derivatized at their reducing ends with the fluorophore 2-aminoacridone. The resulting labeled compounds derived from chondroitin sulfate or heparin/heparan sulfate were well separated and could be quantified by capillary electrophoresis and HPLC with a fluorescence detector. This method was successfully applied to the analysis of the disaccharide composition of sulfated tetra- and hexasaccharides derived from chondroitin sulfate and heparin/heparan sulfate. Remarkably, the saturated and the corresponding unsaturated chondrodisaccharides, the structures of which differ only in their nonreducing terminal uronic acid residues, were also well separated from each other by capillary electrophoresis. This facilitated disaccharide composition analysis of saturated chondrooligosaccharides prepared by testicular hyaluronidase digestion as well as unsaturated chondro- and heparin/heparan sulfate-oligosaccharides prepared by digestion with chondroitinases and heparitinases. The developed method employing the derivatization by 2-aminoacridone in conjunction with capillary electrophoresis or HPLC with a fluorescence detector allows a simple, sensitive, and quantitative disaccharide composition analysis of the glycosaminoglycan-derived oligosaccharides.

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Publisher Full Text

Authors+Show Affiliations

Kitagawa H
Department of Biochemistry, Kobe Pharmaceutical University, Japan.
Kinoshita A
No affiliation info available
Sugahara K
No affiliation info available

MeSH

AminoacridinesChondroitin LyasesChondroitin SulfatesChromatography, High Pressure LiquidCystineDisaccharidesElectrophoresis, CapillaryFluorescent DyesFluorometryGlycosaminoglycansHeparinHeparitin SulfateOligosaccharidesPolysaccharide-Lyases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8600818

Citation

Kitagawa, H, et al. "Microanalysis of Glycosaminoglycan-derived Disaccharides Labeled With the Fluorophore 2-aminoacridone By Capillary Electrophoresis and High-performance Liquid Chromatography." Analytical Biochemistry, vol. 232, no. 1, 1995, pp. 114-21.
Kitagawa H, Kinoshita A, Sugahara K. Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography. Anal Biochem. 1995;232(1):114-21.
Kitagawa, H., Kinoshita, A., & Sugahara, K. (1995). Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography. Analytical Biochemistry, 232(1), 114-21.
Kitagawa H, Kinoshita A, Sugahara K. Microanalysis of Glycosaminoglycan-derived Disaccharides Labeled With the Fluorophore 2-aminoacridone By Capillary Electrophoresis and High-performance Liquid Chromatography. Anal Biochem. 1995 Nov 20;232(1):114-21. PubMed PMID: 8600818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microanalysis of glycosaminoglycan-derived disaccharides labeled with the fluorophore 2-aminoacridone by capillary electrophoresis and high-performance liquid chromatography. AU - Kitagawa,H, AU - Kinoshita,A, AU - Sugahara,K, PY - 1995/11/20/pubmed PY - 1995/11/20/medline PY - 1995/11/20/entrez SP - 114 EP - 21 JF - Analytical biochemistry JO - Anal Biochem VL - 232 IS - 1 N2 - A series of disaccharides derived from chondroitin sulfate and heparin/heparan sulfate were derivatized at their reducing ends with the fluorophore 2-aminoacridone. The resulting labeled compounds derived from chondroitin sulfate or heparin/heparan sulfate were well separated and could be quantified by capillary electrophoresis and HPLC with a fluorescence detector. This method was successfully applied to the analysis of the disaccharide composition of sulfated tetra- and hexasaccharides derived from chondroitin sulfate and heparin/heparan sulfate. Remarkably, the saturated and the corresponding unsaturated chondrodisaccharides, the structures of which differ only in their nonreducing terminal uronic acid residues, were also well separated from each other by capillary electrophoresis. This facilitated disaccharide composition analysis of saturated chondrooligosaccharides prepared by testicular hyaluronidase digestion as well as unsaturated chondro- and heparin/heparan sulfate-oligosaccharides prepared by digestion with chondroitinases and heparitinases. The developed method employing the derivatization by 2-aminoacridone in conjunction with capillary electrophoresis or HPLC with a fluorescence detector allows a simple, sensitive, and quantitative disaccharide composition analysis of the glycosaminoglycan-derived oligosaccharides. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/8600818/Microanalysis_of_glycosaminoglycan_derived_disaccharides_labeled_with_the_fluorophore_2_aminoacridone_by_capillary_electrophoresis_and_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(85)79952-6 DB - PRIME DP - Unbound Medicine ER -
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