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Epstein-Barr virus (EBV) EB1/Zta protein provided in trans and competent for the activation of productive cycle genes does not activate the BZLF1 gene in the EBV genome.
J Gen Virol. 1996 Mar; 77 (Pt 3):501-9.JG

Abstract

The Epstein-Barr Virus (EBV) gene BZLF1 encodes the transcription fact or EB1 (also known as Zta) which is essential for the switch from latency to the lytic cycle: EB1 expressed from a plasmid transfected into B cell lines carrying latent EBV episomes, induces a productive viral cycle. Furthermore, EB1-specific DNA-binding sequences (ZREs) have been found in the promoters of many EBV early genes, including the BZLF1 promoter PZ and the PR promoter. At promoter PR, bicistronic mRNAs are initiated which contain, from 5' to 3', the BRLF1 and the BZLF1 open reading frames (ORFs) encoding respectively the R and EB1 proteins. The current model for the activation of the EBV lytic cycle implies that downregulation of the PZ promoter activity is a key element for latency and that a limiting step in the activation of the productive cycle is the translation of EB1. Once made, EB1 autoactivates promoter PZ, activates the PR promoter at which an mRNA coding for the EBV transcription factor R is initiated and activates the EBV early genes and the ORIlyt, due to unrestricted accessibility of the EB1-responsive elements in the viral genome. We show here that EB1 expressed from a plasmid activated most if not all of the EBV early genes in the viral genome but not its own gene, BZLF1. Moreover, transfected EB1 induced the transcription of the bicistronic mRNAs from which R is efficiently translated but not EB1. Our results demonstrate that EB1 provided in trans, although competent to activate the productive cycle genes, was not sufficient to overcome the downregulation of the PZ promoter.

Authors+Show Affiliations

Unité de virologie humaine, ENS-INSERM, U412, Ecole Normale Supérieure de Lyon, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8601788

Citation

Le Roux, F, et al. "Epstein-Barr Virus (EBV) EB1/Zta Protein Provided in Trans and Competent for the Activation of Productive Cycle Genes Does Not Activate the BZLF1 Gene in the EBV Genome." The Journal of General Virology, vol. 77 (Pt 3), 1996, pp. 501-9.
Le Roux F, Sergeant A, Corbo L. Epstein-Barr virus (EBV) EB1/Zta protein provided in trans and competent for the activation of productive cycle genes does not activate the BZLF1 gene in the EBV genome. J Gen Virol. 1996;77 (Pt 3):501-9.
Le Roux, F., Sergeant, A., & Corbo, L. (1996). Epstein-Barr virus (EBV) EB1/Zta protein provided in trans and competent for the activation of productive cycle genes does not activate the BZLF1 gene in the EBV genome. The Journal of General Virology, 77 (Pt 3), 501-9.
Le Roux F, Sergeant A, Corbo L. Epstein-Barr Virus (EBV) EB1/Zta Protein Provided in Trans and Competent for the Activation of Productive Cycle Genes Does Not Activate the BZLF1 Gene in the EBV Genome. J Gen Virol. 1996;77 (Pt 3):501-9. PubMed PMID: 8601788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus (EBV) EB1/Zta protein provided in trans and competent for the activation of productive cycle genes does not activate the BZLF1 gene in the EBV genome. AU - Le Roux,F, AU - Sergeant,A, AU - Corbo,L, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 501 EP - 9 JF - The Journal of general virology JO - J. Gen. Virol. VL - 77 (Pt 3) N2 - The Epstein-Barr Virus (EBV) gene BZLF1 encodes the transcription fact or EB1 (also known as Zta) which is essential for the switch from latency to the lytic cycle: EB1 expressed from a plasmid transfected into B cell lines carrying latent EBV episomes, induces a productive viral cycle. Furthermore, EB1-specific DNA-binding sequences (ZREs) have been found in the promoters of many EBV early genes, including the BZLF1 promoter PZ and the PR promoter. At promoter PR, bicistronic mRNAs are initiated which contain, from 5' to 3', the BRLF1 and the BZLF1 open reading frames (ORFs) encoding respectively the R and EB1 proteins. The current model for the activation of the EBV lytic cycle implies that downregulation of the PZ promoter activity is a key element for latency and that a limiting step in the activation of the productive cycle is the translation of EB1. Once made, EB1 autoactivates promoter PZ, activates the PR promoter at which an mRNA coding for the EBV transcription factor R is initiated and activates the EBV early genes and the ORIlyt, due to unrestricted accessibility of the EB1-responsive elements in the viral genome. We show here that EB1 expressed from a plasmid activated most if not all of the EBV early genes in the viral genome but not its own gene, BZLF1. Moreover, transfected EB1 induced the transcription of the bicistronic mRNAs from which R is efficiently translated but not EB1. Our results demonstrate that EB1 provided in trans, although competent to activate the productive cycle genes, was not sufficient to overcome the downregulation of the PZ promoter. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/8601788/Epstein_Barr_virus__EBV__EB1/Zta_protein_provided_in_trans_and_competent_for_the_activation_of_productive_cycle_genes_does_not_activate_the_BZLF1_gene_in_the_EBV_genome_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/0022-1317-77-3-501 DB - PRIME DP - Unbound Medicine ER -