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Adenovirus masquerading as microsporidia.
J Parasitol. 1996 Apr; 82(2):316-9.JP

Abstract

Enterocytozoon bieneusi is a microsporidian that causes a severe, debilitating, chronic diarrhea in some patients with the acquired immunodeficiency syndrome. Specific diagnosis of E. bieneusi currently requires an invasive biopsy procedure and time-consuming preparation of specimens for electron microscopy. Our attempts to establish an in vitro culture system using mammalian cell cultures inoculated with duodenal aspirates, biopsy, or both, from 2 infected patients resulted in inadvertent coculture of an adenovirus and E. bieneusi. The adenovirus-infected cells deceptively appeared to contain spores of microsporidia based on light microscopic examination. Transmission electron microscopy revealed only a few microsporidia, but numerous cells infected with an adenovirus that was subsequently identified as adenovirus type 8. We believe that adenovirus infections prevented the cultured cells from supporting the proliferation of E. bieneusi and ultimately destroyed the cell cultures.

Authors+Show Affiliations

National Center for Infectious Diseases, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8604104

Citation

Visvesvara, G S., et al. "Adenovirus Masquerading as Microsporidia." The Journal of Parasitology, vol. 82, no. 2, 1996, pp. 316-9.
Visvesvara GS, Leitch GJ, Wallace S, et al. Adenovirus masquerading as microsporidia. J Parasitol. 1996;82(2):316-9.
Visvesvara, G. S., Leitch, G. J., Wallace, S., Seaba, C., Erdman, D., & Ewing, E. P. (1996). Adenovirus masquerading as microsporidia. The Journal of Parasitology, 82(2), 316-9.
Visvesvara GS, et al. Adenovirus Masquerading as Microsporidia. J Parasitol. 1996;82(2):316-9. PubMed PMID: 8604104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenovirus masquerading as microsporidia. AU - Visvesvara,G S, AU - Leitch,G J, AU - Wallace,S, AU - Seaba,C, AU - Erdman,D, AU - Ewing,E P,Jr PY - 1996/4/1/pubmed PY - 2001/3/28/medline PY - 1996/4/1/entrez SP - 316 EP - 9 JF - The Journal of parasitology JO - J Parasitol VL - 82 IS - 2 N2 - Enterocytozoon bieneusi is a microsporidian that causes a severe, debilitating, chronic diarrhea in some patients with the acquired immunodeficiency syndrome. Specific diagnosis of E. bieneusi currently requires an invasive biopsy procedure and time-consuming preparation of specimens for electron microscopy. Our attempts to establish an in vitro culture system using mammalian cell cultures inoculated with duodenal aspirates, biopsy, or both, from 2 infected patients resulted in inadvertent coculture of an adenovirus and E. bieneusi. The adenovirus-infected cells deceptively appeared to contain spores of microsporidia based on light microscopic examination. Transmission electron microscopy revealed only a few microsporidia, but numerous cells infected with an adenovirus that was subsequently identified as adenovirus type 8. We believe that adenovirus infections prevented the cultured cells from supporting the proliferation of E. bieneusi and ultimately destroyed the cell cultures. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/8604104/Adenovirus_masquerading_as_microsporidia_ L2 - https://medlineplus.gov/hivaidsandinfections.html DB - PRIME DP - Unbound Medicine ER -