Tags

Type your tag names separated by a space and hit enter

Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury.
Biochem Biophys Res Commun 1996; 219(2):486-91BB

Abstract

A cDNA encoding human phospholipid hydroperoxide glutathione peroxidase (PHGPx) was obtained by PCR amplification from human testis cDNA and was inserted into the plasmid pRc/CMV to construct an expression vector for human PHGPx. Guinea pig cell line 104C1 cells were transfected with the expression vector. One of the transfectants, designated 104Cl/O4C, expressed high glutathione peroxidase activity toward dilinoleoyl phosphatidylcholine hydroperoxide and linoleic acid hydroperoxide. Western blot analysis revealed a large amount of protein immunoreactive against anti-PHGPx antibody in the transfectant. When the cells were incubated with these hydroperoxides, the parental cells suffered from serious cell injury, whereas the transfectant was extremely resistant against lipid hydroperoxide-mediated injury.

Authors+Show Affiliations

Institute of Applied Biochemistry, Yagi Memorial Parke, Mitake, Gifu, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8605014

Citation

Yagi, K, et al. "Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase Gene for Protection of Host Cells From Lipid Hydroperoxide-mediated Injury." Biochemical and Biophysical Research Communications, vol. 219, no. 2, 1996, pp. 486-91.
Yagi K, Komura S, Kojima H, et al. Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury. Biochem Biophys Res Commun. 1996;219(2):486-91.
Yagi, K., Komura, S., Kojima, H., Sun, Q., Nagata, N., Ohishi, N., & Nishikimi, M. (1996). Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury. Biochemical and Biophysical Research Communications, 219(2), pp. 486-91.
Yagi K, et al. Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase Gene for Protection of Host Cells From Lipid Hydroperoxide-mediated Injury. Biochem Biophys Res Commun. 1996 Feb 15;219(2):486-91. PubMed PMID: 8605014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury. AU - Yagi,K, AU - Komura,S, AU - Kojima,H, AU - Sun,Q, AU - Nagata,N, AU - Ohishi,N, AU - Nishikimi,M, PY - 1996/2/15/pubmed PY - 1996/2/15/medline PY - 1996/2/15/entrez SP - 486 EP - 91 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 219 IS - 2 N2 - A cDNA encoding human phospholipid hydroperoxide glutathione peroxidase (PHGPx) was obtained by PCR amplification from human testis cDNA and was inserted into the plasmid pRc/CMV to construct an expression vector for human PHGPx. Guinea pig cell line 104C1 cells were transfected with the expression vector. One of the transfectants, designated 104Cl/O4C, expressed high glutathione peroxidase activity toward dilinoleoyl phosphatidylcholine hydroperoxide and linoleic acid hydroperoxide. Western blot analysis revealed a large amount of protein immunoreactive against anti-PHGPx antibody in the transfectant. When the cells were incubated with these hydroperoxides, the parental cells suffered from serious cell injury, whereas the transfectant was extremely resistant against lipid hydroperoxide-mediated injury. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/8605014/Expression_of_human_phospholipid_hydroperoxide_glutathione_peroxidase_gene_for_protection_of_host_cells_from_lipid_hydroperoxide_mediated_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(96)90260-0 DB - PRIME DP - Unbound Medicine ER -