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Gas chromatography with surface ionization detection: a highly sensitive method for determining underivatized codeine and dihydrocodeine in body fluids.
J Chromatogr B Biomed Appl. 1995 Nov 17; 673(2):189-95.JC

Abstract

Underivatized codeine and dihydrocodeine in human plasma and urine have been determined with a high degree of accuracy by capillary gas chromatography (GC) with surface ionization detection (SID). The drugs were extracted with the aid of Sep-Pak C18 cartridges. Recovery of both drugs was > or = 90%. The calibration curves obtained with dimemorfan as an internal standard showed linearity in the range 4.5-72.3 and 3.0-75.5 ng/ml of plasma for codeine and dihydrocodeine, respectively. The detection limit was about 100 pg on column (2.5 ng/ml sample). Codeine was determined quantitatively in plasma and urine obtained from a volunteer who had received 10 mg codeine phosphate orally 3 h before the sampling: the levels were found to be 14.1 and 142 ng/ml, respectively. The present GC-SID method has been compared carefully with GC-NPD (nitrogen-phosphorus detection) using the same extracts; the sensitivity of GC-SID was more than ten times greater than that of GC-NPD, with background noise correspondingly lower.

Authors+Show Affiliations

Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8611952

Citation

Seno, H, et al. "Gas Chromatography With Surface Ionization Detection: a Highly Sensitive Method for Determining Underivatized Codeine and Dihydrocodeine in Body Fluids." Journal of Chromatography. B, Biomedical Applications, vol. 673, no. 2, 1995, pp. 189-95.
Seno H, Hattori H, Kurono S, et al. Gas chromatography with surface ionization detection: a highly sensitive method for determining underivatized codeine and dihydrocodeine in body fluids. J Chromatogr B Biomed Appl. 1995;673(2):189-95.
Seno, H., Hattori, H., Kurono, S., Yamada, T., Kumazawa, T., Ishii, A., & Suzuki, O. (1995). Gas chromatography with surface ionization detection: a highly sensitive method for determining underivatized codeine and dihydrocodeine in body fluids. Journal of Chromatography. B, Biomedical Applications, 673(2), 189-95.
Seno H, et al. Gas Chromatography With Surface Ionization Detection: a Highly Sensitive Method for Determining Underivatized Codeine and Dihydrocodeine in Body Fluids. J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):189-95. PubMed PMID: 8611952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gas chromatography with surface ionization detection: a highly sensitive method for determining underivatized codeine and dihydrocodeine in body fluids. AU - Seno,H, AU - Hattori,H, AU - Kurono,S, AU - Yamada,T, AU - Kumazawa,T, AU - Ishii,A, AU - Suzuki,O, PY - 1995/11/17/pubmed PY - 1995/11/17/medline PY - 1995/11/17/entrez SP - 189 EP - 95 JF - Journal of chromatography. B, Biomedical applications JO - J Chromatogr B Biomed Appl VL - 673 IS - 2 N2 - Underivatized codeine and dihydrocodeine in human plasma and urine have been determined with a high degree of accuracy by capillary gas chromatography (GC) with surface ionization detection (SID). The drugs were extracted with the aid of Sep-Pak C18 cartridges. Recovery of both drugs was > or = 90%. The calibration curves obtained with dimemorfan as an internal standard showed linearity in the range 4.5-72.3 and 3.0-75.5 ng/ml of plasma for codeine and dihydrocodeine, respectively. The detection limit was about 100 pg on column (2.5 ng/ml sample). Codeine was determined quantitatively in plasma and urine obtained from a volunteer who had received 10 mg codeine phosphate orally 3 h before the sampling: the levels were found to be 14.1 and 142 ng/ml, respectively. The present GC-SID method has been compared carefully with GC-NPD (nitrogen-phosphorus detection) using the same extracts; the sensitivity of GC-SID was more than ten times greater than that of GC-NPD, with background noise correspondingly lower. SN - 1572-6495 UR - https://www.unboundmedicine.com/medline/citation/8611952/Gas_chromatography_with_surface_ionization_detection:_a_highly_sensitive_method_for_determining_underivatized_codeine_and_dihydrocodeine_in_body_fluids_ DB - PRIME DP - Unbound Medicine ER -