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Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens.
Proc Natl Acad Sci U S A. 1995 Dec 19; 92(26):12245-9.PN

Abstract

The virulence (vir) genes of Agrobacterium tumefaciens are induced by low-molecular-weight phenolic compounds and monosaccharides through a two-component regulatory system consisting of the VirA and VirG proteins. However, it is not clear how the phenolic compounds are sensed by the VirA/VirG system. We tested the vir-inducing abilities of 15 different phenolic compounds using four wild-type strains of A. tumefaciens--KU12, C58, A6, and Bo542. We analyzed the relationship between structures of the phenolic compounds and levels of vir gene expression in these strains. In strain KU12, vir genes were not induced by phenolic compounds containing 4'-hydroxy, 3'-methoxy, and 5'-methoxy groups, such as acetosyringone, which strongly induced vir genes of the other three strains. On the other hand, vir genes of strain KU12 were induced by phenolic compounds containing only a 4'-hydroxy group, such as 4-hydroxyacetophenone, which did not induce vir genes of the other three strains. The vir genes of strains KU12, A6, and Bo542 were all induced by phenolic compounds containing 4'-hydroxy and 3'-methoxy groups, such as acetovanillone. By transferring different Ti plasmids into isogenic chromosomal backgrounds, we showed that the phenolic-sensing determinant is associated with Ti plasmid. Subcloning of Ti plasmid indicates that the virA locus determines which phenolic compounds can function as vir gene inducers. These results suggest that the VirA protein directly senses the phenolic compounds for vir gene activation.

Authors+Show Affiliations

Department of Microbiology, University of Washington, Seattle 98195-7242, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8618878

Citation

Lee, Y W., et al. "Genetic Evidence for Direct Sensing of Phenolic Compounds By the VirA Protein of Agrobacterium Tumefaciens." Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 26, 1995, pp. 12245-9.
Lee YW, Jin S, Sim WS, et al. Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens. Proc Natl Acad Sci USA. 1995;92(26):12245-9.
Lee, Y. W., Jin, S., Sim, W. S., & Nester, E. W. (1995). Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens. Proceedings of the National Academy of Sciences of the United States of America, 92(26), 12245-9.
Lee YW, et al. Genetic Evidence for Direct Sensing of Phenolic Compounds By the VirA Protein of Agrobacterium Tumefaciens. Proc Natl Acad Sci USA. 1995 Dec 19;92(26):12245-9. PubMed PMID: 8618878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens. AU - Lee,Y W, AU - Jin,S, AU - Sim,W S, AU - Nester,E W, PY - 1995/12/19/pubmed PY - 1995/12/19/medline PY - 1995/12/19/entrez SP - 12245 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 92 IS - 26 N2 - The virulence (vir) genes of Agrobacterium tumefaciens are induced by low-molecular-weight phenolic compounds and monosaccharides through a two-component regulatory system consisting of the VirA and VirG proteins. However, it is not clear how the phenolic compounds are sensed by the VirA/VirG system. We tested the vir-inducing abilities of 15 different phenolic compounds using four wild-type strains of A. tumefaciens--KU12, C58, A6, and Bo542. We analyzed the relationship between structures of the phenolic compounds and levels of vir gene expression in these strains. In strain KU12, vir genes were not induced by phenolic compounds containing 4'-hydroxy, 3'-methoxy, and 5'-methoxy groups, such as acetosyringone, which strongly induced vir genes of the other three strains. On the other hand, vir genes of strain KU12 were induced by phenolic compounds containing only a 4'-hydroxy group, such as 4-hydroxyacetophenone, which did not induce vir genes of the other three strains. The vir genes of strains KU12, A6, and Bo542 were all induced by phenolic compounds containing 4'-hydroxy and 3'-methoxy groups, such as acetovanillone. By transferring different Ti plasmids into isogenic chromosomal backgrounds, we showed that the phenolic-sensing determinant is associated with Ti plasmid. Subcloning of Ti plasmid indicates that the virA locus determines which phenolic compounds can function as vir gene inducers. These results suggest that the VirA protein directly senses the phenolic compounds for vir gene activation. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/8618878/Genetic_evidence_for_direct_sensing_of_phenolic_compounds_by_the_VirA_protein_of_Agrobacterium_tumefaciens_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=8618878 DB - PRIME DP - Unbound Medicine ER -