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Latent N-methyl-D-aspartate receptors in the recurrent excitatory pathway between hippocampal CA1 pyramidal neurons: Ca(2+)-dependent activation by blocking A1 adenosine receptors.
Proc Natl Acad Sci U S A. 1995 Dec 19; 92(26):12431-5.PN

Abstract

When performed at increased external [Ca2+]/[Mg2+] ratio (2.5 mM/0.5 mM), temporary block of A1 adenosine receptors in hippocampus [by 8-cyclopentyltheophylline (CPT)] leads to a dramatic and irreversible change in the excitatory postsynaptic current (EPSC) evoked by Schaffer collateral/commissural (SCC) stimulation and recorded by in situ patch clamp in CA1 pyramidal neurons. The duration of the EPSC becomes stimulus dependent, increasing with increase in stimulus strength. The later occurring component of the EPSC is carried through N-methyl-D-aspartate (NMDA) receptor-operated channels but disappears under either the NMDA antagonist 2-amino-5-phosphonovaleric acid (APV) or the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). These findings indicate that the late component of the SCC-evoked EPSC is polysynaptic: predominantly non-NMDA receptor-mediated SCC inputs excite CA1 neurons that recurrently excite each other by predominantly NDMA receptor-mediated synapses. These recurrent connections are normally silent but become active after CPT treatment, leading to enhancement of the late component of the EPSC. The activity of these connections is maintained for at least 2 hr after CPT removal. When all functional NMDA receptors are blocked by dizocilpine maleate (MK-801), subsequent application of CPT leads to a partial reappearance of NMDA receptor-mediated EPSCs evoked by SCC stimulation, indicating that latent NMDA receptors are recruited. Altogether, these findings indicate the existence of a powerful system of NMDA receptor-mediated synaptic contacts in SCC input to hippocampal CA1 pyramidal neurons and probably also in reciprocal connections between these neurons, which in the usual preparation are kept latent by activity of A1 receptors.

Authors+Show Affiliations

Department of Cellular Membranology, Bogomoletz Institute of Physiology, Kiev, Ukraine.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8618915

Citation

Klishin, A, et al. "Latent N-methyl-D-aspartate Receptors in the Recurrent Excitatory Pathway Between Hippocampal CA1 Pyramidal Neurons: Ca(2+)-dependent Activation By Blocking A1 Adenosine Receptors." Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 26, 1995, pp. 12431-5.
Klishin A, Tsintsadze T, Lozovaya N, et al. Latent N-methyl-D-aspartate receptors in the recurrent excitatory pathway between hippocampal CA1 pyramidal neurons: Ca(2+)-dependent activation by blocking A1 adenosine receptors. Proc Natl Acad Sci U S A. 1995;92(26):12431-5.
Klishin, A., Tsintsadze, T., Lozovaya, N., & Krishtal, O. (1995). Latent N-methyl-D-aspartate receptors in the recurrent excitatory pathway between hippocampal CA1 pyramidal neurons: Ca(2+)-dependent activation by blocking A1 adenosine receptors. Proceedings of the National Academy of Sciences of the United States of America, 92(26), 12431-5.
Klishin A, et al. Latent N-methyl-D-aspartate Receptors in the Recurrent Excitatory Pathway Between Hippocampal CA1 Pyramidal Neurons: Ca(2+)-dependent Activation By Blocking A1 Adenosine Receptors. Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12431-5. PubMed PMID: 8618915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Latent N-methyl-D-aspartate receptors in the recurrent excitatory pathway between hippocampal CA1 pyramidal neurons: Ca(2+)-dependent activation by blocking A1 adenosine receptors. AU - Klishin,A, AU - Tsintsadze,T, AU - Lozovaya,N, AU - Krishtal,O, PY - 1995/12/19/pubmed PY - 1995/12/19/medline PY - 1995/12/19/entrez SP - 12431 EP - 5 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 92 IS - 26 N2 - When performed at increased external [Ca2+]/[Mg2+] ratio (2.5 mM/0.5 mM), temporary block of A1 adenosine receptors in hippocampus [by 8-cyclopentyltheophylline (CPT)] leads to a dramatic and irreversible change in the excitatory postsynaptic current (EPSC) evoked by Schaffer collateral/commissural (SCC) stimulation and recorded by in situ patch clamp in CA1 pyramidal neurons. The duration of the EPSC becomes stimulus dependent, increasing with increase in stimulus strength. The later occurring component of the EPSC is carried through N-methyl-D-aspartate (NMDA) receptor-operated channels but disappears under either the NMDA antagonist 2-amino-5-phosphonovaleric acid (APV) or the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). These findings indicate that the late component of the SCC-evoked EPSC is polysynaptic: predominantly non-NMDA receptor-mediated SCC inputs excite CA1 neurons that recurrently excite each other by predominantly NDMA receptor-mediated synapses. These recurrent connections are normally silent but become active after CPT treatment, leading to enhancement of the late component of the EPSC. The activity of these connections is maintained for at least 2 hr after CPT removal. When all functional NMDA receptors are blocked by dizocilpine maleate (MK-801), subsequent application of CPT leads to a partial reappearance of NMDA receptor-mediated EPSCs evoked by SCC stimulation, indicating that latent NMDA receptors are recruited. Altogether, these findings indicate the existence of a powerful system of NMDA receptor-mediated synaptic contacts in SCC input to hippocampal CA1 pyramidal neurons and probably also in reciprocal connections between these neurons, which in the usual preparation are kept latent by activity of A1 receptors. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/8618915/Latent_N_methyl_D_aspartate_receptors_in_the_recurrent_excitatory_pathway_between_hippocampal_CA1_pyramidal_neurons:_Ca_2+__dependent_activation_by_blocking_A1_adenosine_receptors_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=8618915 DB - PRIME DP - Unbound Medicine ER -