Tags

Type your tag names separated by a space and hit enter

X-ray diffraction analysis of isolated skin lipids: reconstitution of intercellular lipid domains.
Biochemistry. 1996 Mar 26; 35(12):3649-53.B

Abstract

Low- and wide-angle X-ray diffraction were used to determine the structural organization of lipids isolated from the stratum corneum extracellular matrix that forms the major water permeability barrier in mammalian epidermis. Hydrated pig skin ceramides gave a single low-angle reflection of about 62 angstroms and a wide-angle-reflection at 4.15 angstroms. The addition of either cholesterol or fatty acid, the other major lipid components of the skin stratum corneum extracellular matrix, modified this diffraction pattern, depending on the lipid mole ratios. In the absence of water, lipid mixtures exhibited lipid phase separation, as shown by low- and wide-angle reflections typical of a separate cholesterol phase. However, a hydrated 2:1:1 mole ratio of ceramide:cholesterol:palmitic acid (similar to that found in stratum corneum) produced a diffraction pattern with a single sharp wide-angle reflection at 4.10 angstroms and low-angle reflections which indexed as the first eight orders of a single repeat period of 130 angstroms. The repeat period and intensity distribution of the low-angle data were similar to those found in intact stratum corneum [White et al. (1988) Biochemistry 27, 3725-3732; Bouwstra et al. (1994) Biochim. Biophys. Acta 1212, 183-192]. Higher concentrations of cholesterol or palmitic acid resulted in lipid phase separations. The 130 angstrom repeat period decreased only about 3 angstroms as water was removed by incubation in low-relative humidity atmospheres. The 130 angstrom repeat period depended on the presence of a particular ceramide, N-(omega-acyloxy)-acylsphingosine, which is found only in the epidermis. In contrast, 2:1:1 mixtures of brain ceramide:cholesterol:palmitic acid gave reflections of 56 and 34 angstroms. These results indicate that a structure with dimensions similar to those of the lamellar repeating unit found in skin stratum corneum does not depend on the presence of protein but does depend on the presence of specific skin ceramides and appropriate concentrations of cholesterol and fatty acid.

Authors+Show Affiliations

Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8619983

Citation

McIntosh, T J., et al. "X-ray Diffraction Analysis of Isolated Skin Lipids: Reconstitution of Intercellular Lipid Domains." Biochemistry, vol. 35, no. 12, 1996, pp. 3649-53.
McIntosh TJ, Stewart ME, Downing DT. X-ray diffraction analysis of isolated skin lipids: reconstitution of intercellular lipid domains. Biochemistry. 1996;35(12):3649-53.
McIntosh, T. J., Stewart, M. E., & Downing, D. T. (1996). X-ray diffraction analysis of isolated skin lipids: reconstitution of intercellular lipid domains. Biochemistry, 35(12), 3649-53.
McIntosh TJ, Stewart ME, Downing DT. X-ray Diffraction Analysis of Isolated Skin Lipids: Reconstitution of Intercellular Lipid Domains. Biochemistry. 1996 Mar 26;35(12):3649-53. PubMed PMID: 8619983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - X-ray diffraction analysis of isolated skin lipids: reconstitution of intercellular lipid domains. AU - McIntosh,T J, AU - Stewart,M E, AU - Downing,D T, PY - 1996/3/26/pubmed PY - 1996/3/26/medline PY - 1996/3/26/entrez SP - 3649 EP - 53 JF - Biochemistry JO - Biochemistry VL - 35 IS - 12 N2 - Low- and wide-angle X-ray diffraction were used to determine the structural organization of lipids isolated from the stratum corneum extracellular matrix that forms the major water permeability barrier in mammalian epidermis. Hydrated pig skin ceramides gave a single low-angle reflection of about 62 angstroms and a wide-angle-reflection at 4.15 angstroms. The addition of either cholesterol or fatty acid, the other major lipid components of the skin stratum corneum extracellular matrix, modified this diffraction pattern, depending on the lipid mole ratios. In the absence of water, lipid mixtures exhibited lipid phase separation, as shown by low- and wide-angle reflections typical of a separate cholesterol phase. However, a hydrated 2:1:1 mole ratio of ceramide:cholesterol:palmitic acid (similar to that found in stratum corneum) produced a diffraction pattern with a single sharp wide-angle reflection at 4.10 angstroms and low-angle reflections which indexed as the first eight orders of a single repeat period of 130 angstroms. The repeat period and intensity distribution of the low-angle data were similar to those found in intact stratum corneum [White et al. (1988) Biochemistry 27, 3725-3732; Bouwstra et al. (1994) Biochim. Biophys. Acta 1212, 183-192]. Higher concentrations of cholesterol or palmitic acid resulted in lipid phase separations. The 130 angstrom repeat period decreased only about 3 angstroms as water was removed by incubation in low-relative humidity atmospheres. The 130 angstrom repeat period depended on the presence of a particular ceramide, N-(omega-acyloxy)-acylsphingosine, which is found only in the epidermis. In contrast, 2:1:1 mixtures of brain ceramide:cholesterol:palmitic acid gave reflections of 56 and 34 angstroms. These results indicate that a structure with dimensions similar to those of the lamellar repeating unit found in skin stratum corneum does not depend on the presence of protein but does depend on the presence of specific skin ceramides and appropriate concentrations of cholesterol and fatty acid. SN - 0006-2960 UR - https://www.unboundmedicine.com/medline/citation/8619983/X_ray_diffraction_analysis_of_isolated_skin_lipids:_reconstitution_of_intercellular_lipid_domains_ L2 - https://doi.org/10.1021/bi952762q DB - PRIME DP - Unbound Medicine ER -