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Rapid analysis of amphetamines in blood using head space-solid phase microextraction and selected ion monitoring.
Forensic Sci Int. 1996 Apr 02; 78(2):95-102.FS

Abstract

A simple and rapid method for analysis of methamphetamine (MA) and amphetamine (AP) in blood was developed using head space-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry/electron impact ionization-selected ion monitoring (GC-MS/EI-SIM). A vial containing a blood sample, sodium hydroxide, and pentadeuterated methamphetamine as an internal standard, was heated at 80 degrees C for 20 min. The extraction fiber of the SPME was exposed for 5 min in the head space of the vial. First, heptafluorobutyric anhydride solution was injected into the injection port of the GC-MS to make heptafluorobutyramide (HFB) derivatives of amphetamines, and compounds absorbed on the fiber were detached by exposing the fiber in the injection port. Straight calibration curves of MA and AP were obtained from 0.01 to 2 micrograms/g in blood, respectively. No interfering substances were found, and the time for analysis was 30 min for one sample.

Authors+Show Affiliations

Department of Legal Medicine, Hirsohima University School of Medicine, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8621124

Citation

Nagasawa, N, et al. "Rapid Analysis of Amphetamines in Blood Using Head Space-solid Phase Microextraction and Selected Ion Monitoring." Forensic Science International, vol. 78, no. 2, 1996, pp. 95-102.
Nagasawa N, Yashiki M, Iwasaki Y, et al. Rapid analysis of amphetamines in blood using head space-solid phase microextraction and selected ion monitoring. Forensic Sci Int. 1996;78(2):95-102.
Nagasawa, N., Yashiki, M., Iwasaki, Y., Hara, K., & Kojima, T. (1996). Rapid analysis of amphetamines in blood using head space-solid phase microextraction and selected ion monitoring. Forensic Science International, 78(2), 95-102.
Nagasawa N, et al. Rapid Analysis of Amphetamines in Blood Using Head Space-solid Phase Microextraction and Selected Ion Monitoring. Forensic Sci Int. 1996 Apr 2;78(2):95-102. PubMed PMID: 8621124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid analysis of amphetamines in blood using head space-solid phase microextraction and selected ion monitoring. AU - Nagasawa,N, AU - Yashiki,M, AU - Iwasaki,Y, AU - Hara,K, AU - Kojima,T, PY - 1996/4/2/pubmed PY - 1996/4/2/medline PY - 1996/4/2/entrez SP - 95 EP - 102 JF - Forensic science international JO - Forensic Sci. Int. VL - 78 IS - 2 N2 - A simple and rapid method for analysis of methamphetamine (MA) and amphetamine (AP) in blood was developed using head space-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry/electron impact ionization-selected ion monitoring (GC-MS/EI-SIM). A vial containing a blood sample, sodium hydroxide, and pentadeuterated methamphetamine as an internal standard, was heated at 80 degrees C for 20 min. The extraction fiber of the SPME was exposed for 5 min in the head space of the vial. First, heptafluorobutyric anhydride solution was injected into the injection port of the GC-MS to make heptafluorobutyramide (HFB) derivatives of amphetamines, and compounds absorbed on the fiber were detached by exposing the fiber in the injection port. Straight calibration curves of MA and AP were obtained from 0.01 to 2 micrograms/g in blood, respectively. No interfering substances were found, and the time for analysis was 30 min for one sample. SN - 0379-0738 UR - https://www.unboundmedicine.com/medline/citation/8621124/Rapid_analysis_of_amphetamines_in_blood_using_head_space_solid_phase_microextraction_and_selected_ion_monitoring_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0379-0738(95)01874-3 DB - PRIME DP - Unbound Medicine ER -