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Regulation of expression of ob mRNA and protein by glucocorticoids and cAMP.
J Biol Chem. 1996 Mar 08; 271(10):5301-4.JB

Abstract

Regulation of obese gene (ob) expression in ob/ob and db/db mice and in cultured rat adipocytes was examined. It has been demonstrated that exogenous human OB protein (leptin) treatment reduces food intake and weight gain, as well as insulin, glucose, and corticosterone levels in ob/ob mice. In the present report we show that leptin treatment down-regulates endogenous adipose ob mRNA. However, treatment of isolated rat adipocytes with 100 ng/ml human or murine leptin had no direct effect on expression of endogenous ob mRNA, suggesting that leptin may be able to down-regulate its own expression by an indirect, non-autocrine mechanism. Glucocorticoids increased both ob mRNA levels and secreted leptin levels in vitro. Conversely, agents that increase intracellular cAMP, such as beta-adrenergic agonists or Bt2cAMP itself, decreased ob mRNA expression and leptin secretion. Therefore, increased glucocorticoid levels and decreased sympathetic neural activity may contribute to the elevated ob mRNA expression observed in genetically obese, hyperglucocorticoid rodents. Furthermore, leptin might regulate its own expression through a feedback mechanism involving the hypothalamic pituitary axis.

Authors+Show Affiliations

Endocrine Research and Technology Core Divisions, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8621378

Citation

Slieker, L J., et al. "Regulation of Expression of Ob mRNA and Protein By Glucocorticoids and CAMP." The Journal of Biological Chemistry, vol. 271, no. 10, 1996, pp. 5301-4.
Slieker LJ, Sloop KW, Surface PL, et al. Regulation of expression of ob mRNA and protein by glucocorticoids and cAMP. J Biol Chem. 1996;271(10):5301-4.
Slieker, L. J., Sloop, K. W., Surface, P. L., Kriauciunas, A., LaQuier, F., Manetta, J., Bue-Valleskey, J., & Stephens, T. W. (1996). Regulation of expression of ob mRNA and protein by glucocorticoids and cAMP. The Journal of Biological Chemistry, 271(10), 5301-4.
Slieker LJ, et al. Regulation of Expression of Ob mRNA and Protein By Glucocorticoids and CAMP. J Biol Chem. 1996 Mar 8;271(10):5301-4. PubMed PMID: 8621378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of expression of ob mRNA and protein by glucocorticoids and cAMP. AU - Slieker,L J, AU - Sloop,K W, AU - Surface,P L, AU - Kriauciunas,A, AU - LaQuier,F, AU - Manetta,J, AU - Bue-Valleskey,J, AU - Stephens,T W, PY - 1996/3/8/pubmed PY - 1996/3/8/medline PY - 1996/3/8/entrez SP - 5301 EP - 4 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 271 IS - 10 N2 - Regulation of obese gene (ob) expression in ob/ob and db/db mice and in cultured rat adipocytes was examined. It has been demonstrated that exogenous human OB protein (leptin) treatment reduces food intake and weight gain, as well as insulin, glucose, and corticosterone levels in ob/ob mice. In the present report we show that leptin treatment down-regulates endogenous adipose ob mRNA. However, treatment of isolated rat adipocytes with 100 ng/ml human or murine leptin had no direct effect on expression of endogenous ob mRNA, suggesting that leptin may be able to down-regulate its own expression by an indirect, non-autocrine mechanism. Glucocorticoids increased both ob mRNA levels and secreted leptin levels in vitro. Conversely, agents that increase intracellular cAMP, such as beta-adrenergic agonists or Bt2cAMP itself, decreased ob mRNA expression and leptin secretion. Therefore, increased glucocorticoid levels and decreased sympathetic neural activity may contribute to the elevated ob mRNA expression observed in genetically obese, hyperglucocorticoid rodents. Furthermore, leptin might regulate its own expression through a feedback mechanism involving the hypothalamic pituitary axis. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/8621378/Regulation_of_expression_of_ob_mRNA_and_protein_by_glucocorticoids_and_cAMP_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=8621378 DB - PRIME DP - Unbound Medicine ER -