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An automated preconcentration-derivatization system for the determination of cocaine and its metabolites in urine and illicit cocaine samples by gas chromatography/mass spectrometry.
Rapid Commun Mass Spectrom. 1996; 10(6):631-6.RC

Abstract

Although gas chromatography/mass spectrometry (GC/MS) is the most powerful technique for analysing cocaine and its hydrolysis products (benzoylecgonine, ecgonine methyl ester and ecgonine) in urine, the sample pretreatment required is rather laborious and involves a large number of steps. This paper reports a continuous method for the separation (based on the retention properties of cocaine and its major metabolites on a reversed phase C18 column), preconcentration (evaporation under nitrogen) and derivatization (collection of the residue in a few microlitres of derivatizing reagent) of cocaine and its metabolites in urine samples for subsequent analysis by GC/MS. Calibration graphs are linear from 5 to 3000 ng/mL; the detection limits are 1 ng/mL (cocaine and ecgonine methyl ester), 20 ng/mL (benzoylecgonine) and 200 ng/mL (ecgonine). The method was applied to illicit cocaine samples and to urine from a drug addict.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8624416

Citation

Cardenas, S, et al. "An Automated Preconcentration-derivatization System for the Determination of Cocaine and Its Metabolites in Urine and Illicit Cocaine Samples By Gas Chromatography/mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 10, no. 6, 1996, pp. 631-6.
Cardenas S, Gallego M, Valcarcel M. An automated preconcentration-derivatization system for the determination of cocaine and its metabolites in urine and illicit cocaine samples by gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 1996;10(6):631-6.
Cardenas, S., Gallego, M., & Valcarcel, M. (1996). An automated preconcentration-derivatization system for the determination of cocaine and its metabolites in urine and illicit cocaine samples by gas chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 10(6), 631-6.
Cardenas S, Gallego M, Valcarcel M. An Automated Preconcentration-derivatization System for the Determination of Cocaine and Its Metabolites in Urine and Illicit Cocaine Samples By Gas Chromatography/mass Spectrometry. Rapid Commun Mass Spectrom. 1996;10(6):631-6. PubMed PMID: 8624416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An automated preconcentration-derivatization system for the determination of cocaine and its metabolites in urine and illicit cocaine samples by gas chromatography/mass spectrometry. AU - Cardenas,S, AU - Gallego,M, AU - Valcarcel,M, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 631 EP - 6 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 10 IS - 6 N2 - Although gas chromatography/mass spectrometry (GC/MS) is the most powerful technique for analysing cocaine and its hydrolysis products (benzoylecgonine, ecgonine methyl ester and ecgonine) in urine, the sample pretreatment required is rather laborious and involves a large number of steps. This paper reports a continuous method for the separation (based on the retention properties of cocaine and its major metabolites on a reversed phase C18 column), preconcentration (evaporation under nitrogen) and derivatization (collection of the residue in a few microlitres of derivatizing reagent) of cocaine and its metabolites in urine samples for subsequent analysis by GC/MS. Calibration graphs are linear from 5 to 3000 ng/mL; the detection limits are 1 ng/mL (cocaine and ecgonine methyl ester), 20 ng/mL (benzoylecgonine) and 200 ng/mL (ecgonine). The method was applied to illicit cocaine samples and to urine from a drug addict. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/8624416/An_automated_preconcentration_derivatization_system_for_the_determination_of_cocaine_and_its_metabolites_in_urine_and_illicit_cocaine_samples_by_gas_chromatography/mass_spectrometry_ L2 - https://doi.org/10.1002/(SICI)1097-0231(199604)10:6<631::AID-RCM524>3.0.CO;2-T DB - PRIME DP - Unbound Medicine ER -