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Sequence divergence associated with species-specific splicing of the nonmuscle beta-tropomyosin alternative exon.
J Biol Chem. 1996 May 10; 271(19):11511-7.JB

Abstract

Alternative splicing of vertebrate beta-tropomyosin transcripts ensures mutually exclusive expression of internal exons 6A and 6B in nonmuscle and skeletal muscle cells, respectively. Recently, we reported that this splicing regulation requires species-specific elements, since the splicing profile for the chicken, rat, and Xenopus beta-tropomyosin alternative exons is not reproduced in transfection experiments when heterologous myogenic cells are used. By analyzing the splicing pattern of hybrid chicken/rat beta-TM constructions transfected into both quail and mouse cell lines, we demonstrate that chicken beta-tropomyosin exon 6A is flanked by stronger splicing signals than rat exon 6A, thus leading to the misregulation of splicing in heterologous cells. We have characterized three splicing signals that contribute to this difference: 1) nonconsensus nucleotide differences at positions +4 and +6 in the donor site downstream of exon 6A, 2) differences in the pyrimidine composition between the branch site and acceptor site upstream of exon 6A, and 3) a pyrimidine-rich intronic exon 6A splicing enhancer present upstream of exon 6A only in the chicken beta-TM gene. The functional divergence between splicing signals in two homologous vertebrate genes reveals species-specific strategies for proper modulation of splicing of alternative exons.

Authors+Show Affiliations

Department of Molecular Biology, Pasteur Institute, Paris, France.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8626711

Citation

Pret, A M., and M Y. Fiszman. "Sequence Divergence Associated With Species-specific Splicing of the Nonmuscle Beta-tropomyosin Alternative Exon." The Journal of Biological Chemistry, vol. 271, no. 19, 1996, pp. 11511-7.
Pret AM, Fiszman MY. Sequence divergence associated with species-specific splicing of the nonmuscle beta-tropomyosin alternative exon. J Biol Chem. 1996;271(19):11511-7.
Pret, A. M., & Fiszman, M. Y. (1996). Sequence divergence associated with species-specific splicing of the nonmuscle beta-tropomyosin alternative exon. The Journal of Biological Chemistry, 271(19), 11511-7.
Pret AM, Fiszman MY. Sequence Divergence Associated With Species-specific Splicing of the Nonmuscle Beta-tropomyosin Alternative Exon. J Biol Chem. 1996 May 10;271(19):11511-7. PubMed PMID: 8626711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence divergence associated with species-specific splicing of the nonmuscle beta-tropomyosin alternative exon. AU - Pret,A M, AU - Fiszman,M Y, PY - 1996/5/10/pubmed PY - 1996/5/10/medline PY - 1996/5/10/entrez SP - 11511 EP - 7 JF - The Journal of biological chemistry JO - J Biol Chem VL - 271 IS - 19 N2 - Alternative splicing of vertebrate beta-tropomyosin transcripts ensures mutually exclusive expression of internal exons 6A and 6B in nonmuscle and skeletal muscle cells, respectively. Recently, we reported that this splicing regulation requires species-specific elements, since the splicing profile for the chicken, rat, and Xenopus beta-tropomyosin alternative exons is not reproduced in transfection experiments when heterologous myogenic cells are used. By analyzing the splicing pattern of hybrid chicken/rat beta-TM constructions transfected into both quail and mouse cell lines, we demonstrate that chicken beta-tropomyosin exon 6A is flanked by stronger splicing signals than rat exon 6A, thus leading to the misregulation of splicing in heterologous cells. We have characterized three splicing signals that contribute to this difference: 1) nonconsensus nucleotide differences at positions +4 and +6 in the donor site downstream of exon 6A, 2) differences in the pyrimidine composition between the branch site and acceptor site upstream of exon 6A, and 3) a pyrimidine-rich intronic exon 6A splicing enhancer present upstream of exon 6A only in the chicken beta-TM gene. The functional divergence between splicing signals in two homologous vertebrate genes reveals species-specific strategies for proper modulation of splicing of alternative exons. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/8626711/Sequence_divergence_associated_with_species_specific_splicing_of_the_nonmuscle_beta_tropomyosin_alternative_exon_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(17)47020-7 DB - PRIME DP - Unbound Medicine ER -