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Activity of gastric mucosal nitric oxide synthase in portal hypertensive gastropathy.
Am J Gastroenterol. 1996 Mar; 91(3):535-8.AJ

Abstract

OBJECTIVE

The importance of portal hypertensive gastropathy, as a potentially bleeding lesion in cirrhotics with portal hypertension, has recently been appreciated. Histologically, dilation of the mucosal and submucosal vessels of the stomach is noted in this entity. The possibility of nitric oxide acting as a mediator for this mucosal vascular dilation has not been explored.

METHODS

We determined, in a group of 10 male cirrhotic patients with esophageal varices and endoscopic changes consistent with severe portal hypertensive gastropathy (Group A), the gastric mucosal nitric oxide synthase activity. This was determined by measuring the rate of conversion of [3H]-arginine to [3H]-citrulline. Serum levels of nitrates and nitrites, the end products of nitric oxide, were also measured. The results were compared with those of a group of 10 male controls with no liver disease (Group B).

RESULTS

Gastric mucosal constitutive and inducible nitric oxide synthase levels were significantly higher in group A (125.4 +/- 4.3 and 259.7 +/- 5.5 pmol/mg protein/minute, respectively) than in group B (88 +/- 8.6 and 130.8 +/- 6.6 pmol/mg protein/minute, respectively) (p < 0.002 and < 0.0001, respectively). Serum nitrate/nitrite levels were 30.1 +/- 3.2 nmol/ml in group A and 15.5 +/- 0.09 nmol/ml in group B (p < 0.001).

CONCLUSIONS

We conclude that the significantly increased gastric mucosal nitric oxide synthase activity, in patients with portal hypertensive gastropathy, suggests an important role for nitric oxide in the pathogenesis of this mucosal lesion.

Authors+Show Affiliations

Division of Digestive Diseases, Veteran Affairs Medical Center, Jackson, Mississippi, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8633504

Citation

El-Newihi, H M., et al. "Activity of Gastric Mucosal Nitric Oxide Synthase in Portal Hypertensive Gastropathy." The American Journal of Gastroenterology, vol. 91, no. 3, 1996, pp. 535-8.
El-Newihi HM, Kanji VK, Mihas AA. Activity of gastric mucosal nitric oxide synthase in portal hypertensive gastropathy. Am J Gastroenterol. 1996;91(3):535-8.
El-Newihi, H. M., Kanji, V. K., & Mihas, A. A. (1996). Activity of gastric mucosal nitric oxide synthase in portal hypertensive gastropathy. The American Journal of Gastroenterology, 91(3), 535-8.
El-Newihi HM, Kanji VK, Mihas AA. Activity of Gastric Mucosal Nitric Oxide Synthase in Portal Hypertensive Gastropathy. Am J Gastroenterol. 1996;91(3):535-8. PubMed PMID: 8633504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity of gastric mucosal nitric oxide synthase in portal hypertensive gastropathy. AU - El-Newihi,H M, AU - Kanji,V K, AU - Mihas,A A, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 535 EP - 8 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 91 IS - 3 N2 - OBJECTIVE: The importance of portal hypertensive gastropathy, as a potentially bleeding lesion in cirrhotics with portal hypertension, has recently been appreciated. Histologically, dilation of the mucosal and submucosal vessels of the stomach is noted in this entity. The possibility of nitric oxide acting as a mediator for this mucosal vascular dilation has not been explored. METHODS: We determined, in a group of 10 male cirrhotic patients with esophageal varices and endoscopic changes consistent with severe portal hypertensive gastropathy (Group A), the gastric mucosal nitric oxide synthase activity. This was determined by measuring the rate of conversion of [3H]-arginine to [3H]-citrulline. Serum levels of nitrates and nitrites, the end products of nitric oxide, were also measured. The results were compared with those of a group of 10 male controls with no liver disease (Group B). RESULTS: Gastric mucosal constitutive and inducible nitric oxide synthase levels were significantly higher in group A (125.4 +/- 4.3 and 259.7 +/- 5.5 pmol/mg protein/minute, respectively) than in group B (88 +/- 8.6 and 130.8 +/- 6.6 pmol/mg protein/minute, respectively) (p < 0.002 and < 0.0001, respectively). Serum nitrate/nitrite levels were 30.1 +/- 3.2 nmol/ml in group A and 15.5 +/- 0.09 nmol/ml in group B (p < 0.001). CONCLUSIONS: We conclude that the significantly increased gastric mucosal nitric oxide synthase activity, in patients with portal hypertensive gastropathy, suggests an important role for nitric oxide in the pathogenesis of this mucosal lesion. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/8633504/Activity_of_gastric_mucosal_nitric_oxide_synthase_in_portal_hypertensive_gastropathy_ DB - PRIME DP - Unbound Medicine ER -