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Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non-Hodgkin's lymphoma of human immunodeficiency virus-negative Japanese.
Am J Hematol. 1996 May; 52(1):21-8.AJ

Abstract

Sixty cases of B-cell nodal non-Hodgkin's malignant lymphoma (B-ML), and 46 cases of T-cell nodal lymphoma (T-ML) were surveyed for Epstein-Barr virus (EBV) genomes, RNA, and associated proteins. We used a Southern blot analysis, polymerase chain reaction (PCR), and EBV-encoded small RNA-1 (EBER-1) in situ hybridization to investigate the presence of EBV. We performed an immunohistochemical study on EBV-related oncoproteins, such as EBV-determined nuclear antigen-2 (EBNA-2), latent membrane protein (LMP), and viral interleukin-10 (vIL-10). In addition, we also analyzed the terminal repetitive sequence of EBV (EBV-TR) to investigate the EBV-infected cell clonality. Non-Hodgkin's lymphomas were grouped into three types by number of EBV-infected cells: I) almost all lymphoma cells showed an EBV presence; II) some scattered lymphoma cells showed an EBV presence; and III) only a few cells showed such a presence, which was probably due to a latent EBV infection. In 25 of 60 B-MLs, EBV-infected cells were found; 7 were type I, 1 was type II, and 17 were type III. In 27 of 46 T-MLs, EBV-infected cells were found; no cases were type I, 5 cases were type II, and 22 cases were type III. Seven B-MLs and 3 T cell lymphomas showed clonal TR bands. Expression of EBNA-2 was found in only three B-MLs, whereas LMP was seen in four B-MLs and six T-MLs. All EBNA-2/LMP-positive cases showed an EBV presence. In B-MLs, expression of EBNA-2 and LMP was detected in almost all lymphoma cells; in T-MLs, however, LMP was found in only a small portion of the lymphoma cells. Expression of IL-10 was closely associated with LMP. In summary, it was thus speculated that EBV infection was associated with the various states of lymphomagenesis.

Authors+Show Affiliations

Department of Pathology, School of Medicine, Fukuoka University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8638607

Citation

Ohshima, K, et al. "Epstein-Barr Virus Infection and Associated Products (LMP, EBNA2, vIL-10) in Nodal non-Hodgkin's Lymphoma of Human Immunodeficiency Virus-negative Japanese." American Journal of Hematology, vol. 52, no. 1, 1996, pp. 21-8.
Ohshima K, Suzumiya J, Tasiro K, et al. Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non-Hodgkin's lymphoma of human immunodeficiency virus-negative Japanese. Am J Hematol. 1996;52(1):21-8.
Ohshima, K., Suzumiya, J., Tasiro, K., Mukai, Y., Tanaka, T., Kato, A., & Kikuchi, M. (1996). Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non-Hodgkin's lymphoma of human immunodeficiency virus-negative Japanese. American Journal of Hematology, 52(1), 21-8.
Ohshima K, et al. Epstein-Barr Virus Infection and Associated Products (LMP, EBNA2, vIL-10) in Nodal non-Hodgkin's Lymphoma of Human Immunodeficiency Virus-negative Japanese. Am J Hematol. 1996;52(1):21-8. PubMed PMID: 8638607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus infection and associated products (LMP, EBNA2, vIL-10) in nodal non-Hodgkin's lymphoma of human immunodeficiency virus-negative Japanese. AU - Ohshima,K, AU - Suzumiya,J, AU - Tasiro,K, AU - Mukai,Y, AU - Tanaka,T, AU - Kato,A, AU - Kikuchi,M, PY - 1996/5/1/pubmed PY - 2000/6/20/medline PY - 1996/5/1/entrez SP - 21 EP - 8 JF - American journal of hematology JO - Am. J. Hematol. VL - 52 IS - 1 N2 - Sixty cases of B-cell nodal non-Hodgkin's malignant lymphoma (B-ML), and 46 cases of T-cell nodal lymphoma (T-ML) were surveyed for Epstein-Barr virus (EBV) genomes, RNA, and associated proteins. We used a Southern blot analysis, polymerase chain reaction (PCR), and EBV-encoded small RNA-1 (EBER-1) in situ hybridization to investigate the presence of EBV. We performed an immunohistochemical study on EBV-related oncoproteins, such as EBV-determined nuclear antigen-2 (EBNA-2), latent membrane protein (LMP), and viral interleukin-10 (vIL-10). In addition, we also analyzed the terminal repetitive sequence of EBV (EBV-TR) to investigate the EBV-infected cell clonality. Non-Hodgkin's lymphomas were grouped into three types by number of EBV-infected cells: I) almost all lymphoma cells showed an EBV presence; II) some scattered lymphoma cells showed an EBV presence; and III) only a few cells showed such a presence, which was probably due to a latent EBV infection. In 25 of 60 B-MLs, EBV-infected cells were found; 7 were type I, 1 was type II, and 17 were type III. In 27 of 46 T-MLs, EBV-infected cells were found; no cases were type I, 5 cases were type II, and 22 cases were type III. Seven B-MLs and 3 T cell lymphomas showed clonal TR bands. Expression of EBNA-2 was found in only three B-MLs, whereas LMP was seen in four B-MLs and six T-MLs. All EBNA-2/LMP-positive cases showed an EBV presence. In B-MLs, expression of EBNA-2 and LMP was detected in almost all lymphoma cells; in T-MLs, however, LMP was found in only a small portion of the lymphoma cells. Expression of IL-10 was closely associated with LMP. In summary, it was thus speculated that EBV infection was associated with the various states of lymphomagenesis. SN - 0361-8609 UR - https://www.unboundmedicine.com/medline/citation/8638607/Epstein_Barr_virus_infection_and_associated_products__LMP_EBNA2_vIL_10__in_nodal_non_Hodgkin's_lymphoma_of_human_immunodeficiency_virus_negative_Japanese_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0361-8609&date=1996&volume=52&issue=1&spage=21 DB - PRIME DP - Unbound Medicine ER -