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Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein.
Cancer Res. 1996 Mar 01; 56(5):1063-7.CR

Abstract

A plasmid containing the CCAAT/enhancer-binding protein (C/EBP alpha) gene transcriptionally controlled by the metallothionein promoter was constructed. The gene was transfected into the human hepatocellular carcinoma cell lines Hep3B and HepG2. When cultured in vitro in the presence of 100 microM ZnSO(4), C/EBP alpha expression caused reversible growth arrest. In soft agar clonogenic assays, C/EBP alpha expression decreased both the colony size and the total number of colonies compared with zinc-free controls. C/EBP alpha expressing cells s.c. implanted in CD-1 nu/nu mice were essentially nontumorigenic, whereas C/EBP alpha tumor cells implanted into immunodeficient SCID mice demonstrated a significantly delayed time of tumor appearance compared with cells transfected with a vector control plasmid. These studies suggest that the expression of endogenous genes normally associated with a quiescent, differentiated state, such as C/EBP alpha, can result in impaired proliferative activity and suppressed tumorigenicity of hepatoma cell lines.

Authors+Show Affiliations

Division of Cell Biology, Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8640762

Citation

Watkins, P J., et al. "Impaired Proliferation and Tumorigenicity Induced By CCAAT/enhancer-binding Protein." Cancer Research, vol. 56, no. 5, 1996, pp. 1063-7.
Watkins PJ, Condreay JP, Huber BE, et al. Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. Cancer Res. 1996;56(5):1063-7.
Watkins, P. J., Condreay, J. P., Huber, B. E., Jacobs, S. J., & Adams, D. J. (1996). Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. Cancer Research, 56(5), 1063-7.
Watkins PJ, et al. Impaired Proliferation and Tumorigenicity Induced By CCAAT/enhancer-binding Protein. Cancer Res. 1996 Mar 1;56(5):1063-7. PubMed PMID: 8640762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired proliferation and tumorigenicity induced by CCAAT/enhancer-binding protein. AU - Watkins,P J, AU - Condreay,J P, AU - Huber,B E, AU - Jacobs,S J, AU - Adams,D J, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 1063 EP - 7 JF - Cancer research JO - Cancer Res VL - 56 IS - 5 N2 - A plasmid containing the CCAAT/enhancer-binding protein (C/EBP alpha) gene transcriptionally controlled by the metallothionein promoter was constructed. The gene was transfected into the human hepatocellular carcinoma cell lines Hep3B and HepG2. When cultured in vitro in the presence of 100 microM ZnSO(4), C/EBP alpha expression caused reversible growth arrest. In soft agar clonogenic assays, C/EBP alpha expression decreased both the colony size and the total number of colonies compared with zinc-free controls. C/EBP alpha expressing cells s.c. implanted in CD-1 nu/nu mice were essentially nontumorigenic, whereas C/EBP alpha tumor cells implanted into immunodeficient SCID mice demonstrated a significantly delayed time of tumor appearance compared with cells transfected with a vector control plasmid. These studies suggest that the expression of endogenous genes normally associated with a quiescent, differentiated state, such as C/EBP alpha, can result in impaired proliferative activity and suppressed tumorigenicity of hepatoma cell lines. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/8640762/Impaired_proliferation_and_tumorigenicity_induced_by_CCAAT/enhancer_binding_protein_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=8640762 DB - PRIME DP - Unbound Medicine ER -