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The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE).
EMBO J. 1996 May 01; 15(9):2227-35.EJ

Abstract

The MSN2 and MSN4 genes encode homologous and functionally redundant Cys2His2 zinc finger proteins. A disruption of both MSN2 and MSN4 genes results in a higher sensitivity to different stresses, including carbon source starvation, heat shock and severe osmotic and oxidative stresses. We show that MSN2 and MSN4 are required for activation of several yeast genes such as CTT1, DDR2 and HSP12, whose induction is mediated through stress-response elements (STREs). Msn2p and Msn4p are important factors for the stress-induced activation of STRE dependent promoters and bind specifically to STRE-containing oligonucleotides. Our results suggest that MSN2 and MSN4 encode a DNA-binding component of the stress responsive system and it is likely that they act as positive transcription factors.

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Authors+Show Affiliations

Martínez-Pastor MT
Departamento de Bioquímica y Biología Molecular, Universitat de Valencia, Spain.
Marchler G
No affiliation info available
Schüller C
No affiliation info available
Marchler-Bauer A
No affiliation info available
Ruis H
No affiliation info available
Estruch F
No affiliation info available

MeSH

Base SequenceDNA-Binding ProteinsHot TemperatureMolecular Sequence DataOligodeoxyribonucleotidesOsmotic PressureOxidative StressProtein BindingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsTranscription, GeneticZinc Fingers

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8641288

Citation

Martínez-Pastor, M T., et al. "The Saccharomyces Cerevisiae Zinc Finger Proteins Msn2p and Msn4p Are Required for Transcriptional Induction Through the Stress Response Element (STRE)." The EMBO Journal, vol. 15, no. 9, 1996, pp. 2227-35.
Martínez-Pastor MT, Marchler G, Schüller C, et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). EMBO J. 1996;15(9):2227-35.
Martínez-Pastor, M. T., Marchler, G., Schüller, C., Marchler-Bauer, A., Ruis, H., & Estruch, F. (1996). The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). The EMBO Journal, 15(9), 2227-35.
Martínez-Pastor MT, et al. The Saccharomyces Cerevisiae Zinc Finger Proteins Msn2p and Msn4p Are Required for Transcriptional Induction Through the Stress Response Element (STRE). EMBO J. 1996 May 1;15(9):2227-35. PubMed PMID: 8641288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). AU - Martínez-Pastor,M T, AU - Marchler,G, AU - Schüller,C, AU - Marchler-Bauer,A, AU - Ruis,H, AU - Estruch,F, PY - 1996/5/1/pubmed PY - 1996/5/1/medline PY - 1996/5/1/entrez SP - 2227 EP - 35 JF - The EMBO journal JO - EMBO J VL - 15 IS - 9 N2 - The MSN2 and MSN4 genes encode homologous and functionally redundant Cys2His2 zinc finger proteins. A disruption of both MSN2 and MSN4 genes results in a higher sensitivity to different stresses, including carbon source starvation, heat shock and severe osmotic and oxidative stresses. We show that MSN2 and MSN4 are required for activation of several yeast genes such as CTT1, DDR2 and HSP12, whose induction is mediated through stress-response elements (STREs). Msn2p and Msn4p are important factors for the stress-induced activation of STRE dependent promoters and bind specifically to STRE-containing oligonucleotides. Our results suggest that MSN2 and MSN4 encode a DNA-binding component of the stress responsive system and it is likely that they act as positive transcription factors. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/8641288/The_Saccharomyces_cerevisiae_zinc_finger_proteins_Msn2p_and_Msn4p_are_required_for_transcriptional_induction_through_the_stress_response_element__STRE__ DB - PRIME DP - Unbound Medicine ER -