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Aberrant growth of granulocyte-macrophage progenitors in juvenile chronic myelogenous leukemia in serum-free culture.
Exp Hematol. 1996 Feb; 24(2):116-22.EH

Abstract

We investigated the properties of granulocyte-macrophage (GM) progenitors obtained from patients with juvenile chronic myelogenous leukemia (JCML). CD34+ bone marrow cells from a patient with JCML, unlike normal bone marrow cells, generated a large number of cells in serum-containing liquid culture without additional hematopoietic factors. In serum-deprived culture, only granulocyte colony-stimulating factor (G-CSF) had a modest stimulatory effect on GM colony growth in normal controls. In contrast, stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3), as well as G-CSF, when tested individually, generated significant numbers of GM colonies in some JCML patients. All two-factor combinations generated significantly more GM colonies in JCML compared with normal controls. In particular, GM-CSF plus SCF exerted an interaction equivalent to the all-factor combination in most patients. Significant differences in the size and constituent cells of GM colonies stimulated by GM-CSF plus SCF were also observed. These results suggest that one possible mechanism for the excessive cell production in JCML is the strong proliferation of GM progenitors induced by hematopoietic factors, especially SCF. According to immunofluorescent analysis, however, it is unlikely that this multiplication is due to an increase in the cell surface expression of c-kit receptors on JCML progenitors.

Authors+Show Affiliations

Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8641332

Citation

Sawai, N, et al. "Aberrant Growth of Granulocyte-macrophage Progenitors in Juvenile Chronic Myelogenous Leukemia in Serum-free Culture." Experimental Hematology, vol. 24, no. 2, 1996, pp. 116-22.
Sawai N, Koike K, Ito S, et al. Aberrant growth of granulocyte-macrophage progenitors in juvenile chronic myelogenous leukemia in serum-free culture. Exp Hematol. 1996;24(2):116-22.
Sawai, N., Koike, K., Ito, S., Okumura, N., Kamijo, T., Shiohara, M., Amano, Y., Tsuji, K., Nakahata, T., & Oda, M. (1996). Aberrant growth of granulocyte-macrophage progenitors in juvenile chronic myelogenous leukemia in serum-free culture. Experimental Hematology, 24(2), 116-22.
Sawai N, et al. Aberrant Growth of Granulocyte-macrophage Progenitors in Juvenile Chronic Myelogenous Leukemia in Serum-free Culture. Exp Hematol. 1996;24(2):116-22. PubMed PMID: 8641332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aberrant growth of granulocyte-macrophage progenitors in juvenile chronic myelogenous leukemia in serum-free culture. AU - Sawai,N, AU - Koike,K, AU - Ito,S, AU - Okumura,N, AU - Kamijo,T, AU - Shiohara,M, AU - Amano,Y, AU - Tsuji,K, AU - Nakahata,T, AU - Oda,M, PY - 1996/2/1/pubmed PY - 1996/2/1/medline PY - 1996/2/1/entrez SP - 116 EP - 22 JF - Experimental hematology JO - Exp Hematol VL - 24 IS - 2 N2 - We investigated the properties of granulocyte-macrophage (GM) progenitors obtained from patients with juvenile chronic myelogenous leukemia (JCML). CD34+ bone marrow cells from a patient with JCML, unlike normal bone marrow cells, generated a large number of cells in serum-containing liquid culture without additional hematopoietic factors. In serum-deprived culture, only granulocyte colony-stimulating factor (G-CSF) had a modest stimulatory effect on GM colony growth in normal controls. In contrast, stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3), as well as G-CSF, when tested individually, generated significant numbers of GM colonies in some JCML patients. All two-factor combinations generated significantly more GM colonies in JCML compared with normal controls. In particular, GM-CSF plus SCF exerted an interaction equivalent to the all-factor combination in most patients. Significant differences in the size and constituent cells of GM colonies stimulated by GM-CSF plus SCF were also observed. These results suggest that one possible mechanism for the excessive cell production in JCML is the strong proliferation of GM progenitors induced by hematopoietic factors, especially SCF. According to immunofluorescent analysis, however, it is unlikely that this multiplication is due to an increase in the cell surface expression of c-kit receptors on JCML progenitors. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/8641332/Aberrant_growth_of_granulocyte_macrophage_progenitors_in_juvenile_chronic_myelogenous_leukemia_in_serum_free_culture_ L2 - https://medlineplus.gov/chronicmyeloidleukemia.html DB - PRIME DP - Unbound Medicine ER -