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Intraepithelial gamma/delta T cells in duodenal mucosa are related to the immune state and survival time in AIDS.
J Virol. 1996 Jun; 70(6):3545-50.JV

Abstract

The proportion of T-cell receptor gamma/delta+ cells and the CD4/CD8 ratio relative to all CD3+ intraepithelial lymphocytes (IEL) were determined by immunofluorescence in duodenal mucosa of late-stage (mostly CDC IVC1/D) subjects (n = 21) infected with human immunodeficiency virus type 1 (HIV-1). The gamma/delta fraction (median, 14.2%; range, 1.7 to 59.8%) was increased (P < 0.03) compared with that in HIV- controls (n = 11; median 2.8%; range, 0.3 to 38%). Also, the number of gamma/delta+ IEL per mucosal unit was increased (P < 0.05) in the HIV+ subjects (median, 11.1/U) compared with the controls (3.2/U). Approximately 100% of the gamma/delta+ IEL were CD8-, and most expressed the Vdelta1vJdelta1-encoded epitope (median, 90.9%). The total number of CD3+ IEL tended to be lower than in the controls (67.4 versus 72.9/U). Both the epithelium and the lamina propria contained mainly CD8+ T cells, the median ratios of CD4+ T cells being 1 and 7.6%, respectively. This result accorded with the reduced CD4 cell number in blood (median, 18 X 10(6)/liter). The HIV+ subjects had increased serum levels of neopterin and beta2-microglobulin (both P < 0.0001), probably reflecting immunostimulation. Serum neopterin and beta2-microglobulin were inversely related to duodenal gamma/delta IEL, particularly in the premortal group (r = -0.97 and r = -0.58, respectively). The increased gamma/delta IEL might reflect enhanced intestinal protection in late-phase HIV infection. Short survival expectancy (<7 months) was associated not only with high levels of neopterin and beta2-microglobulin but also with a reduced number of duodenal gamma/delta+ cells (P < 0.03).

Authors+Show Affiliations

Laboratory for Immunohistochemistry and Immunopathology, Institute of Pathology, University of Oslo, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8648688

Citation

Nilssen, D E., et al. "Intraepithelial Gamma/delta T Cells in Duodenal Mucosa Are Related to the Immune State and Survival Time in AIDS." Journal of Virology, vol. 70, no. 6, 1996, pp. 3545-50.
Nilssen DE, Müller F, Oktedalen O, et al. Intraepithelial gamma/delta T cells in duodenal mucosa are related to the immune state and survival time in AIDS. J Virol. 1996;70(6):3545-50.
Nilssen, D. E., Müller, F., Oktedalen, O., Frøland, S. S., Fausa, O., Halstensen, T. S., & Brandtzaeg, P. (1996). Intraepithelial gamma/delta T cells in duodenal mucosa are related to the immune state and survival time in AIDS. Journal of Virology, 70(6), 3545-50.
Nilssen DE, et al. Intraepithelial Gamma/delta T Cells in Duodenal Mucosa Are Related to the Immune State and Survival Time in AIDS. J Virol. 1996;70(6):3545-50. PubMed PMID: 8648688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraepithelial gamma/delta T cells in duodenal mucosa are related to the immune state and survival time in AIDS. AU - Nilssen,D E, AU - Müller,F, AU - Oktedalen,O, AU - Frøland,S S, AU - Fausa,O, AU - Halstensen,T S, AU - Brandtzaeg,P, PY - 1996/6/1/pubmed PY - 1996/6/1/medline PY - 1996/6/1/entrez SP - 3545 EP - 50 JF - Journal of virology JO - J Virol VL - 70 IS - 6 N2 - The proportion of T-cell receptor gamma/delta+ cells and the CD4/CD8 ratio relative to all CD3+ intraepithelial lymphocytes (IEL) were determined by immunofluorescence in duodenal mucosa of late-stage (mostly CDC IVC1/D) subjects (n = 21) infected with human immunodeficiency virus type 1 (HIV-1). The gamma/delta fraction (median, 14.2%; range, 1.7 to 59.8%) was increased (P < 0.03) compared with that in HIV- controls (n = 11; median 2.8%; range, 0.3 to 38%). Also, the number of gamma/delta+ IEL per mucosal unit was increased (P < 0.05) in the HIV+ subjects (median, 11.1/U) compared with the controls (3.2/U). Approximately 100% of the gamma/delta+ IEL were CD8-, and most expressed the Vdelta1vJdelta1-encoded epitope (median, 90.9%). The total number of CD3+ IEL tended to be lower than in the controls (67.4 versus 72.9/U). Both the epithelium and the lamina propria contained mainly CD8+ T cells, the median ratios of CD4+ T cells being 1 and 7.6%, respectively. This result accorded with the reduced CD4 cell number in blood (median, 18 X 10(6)/liter). The HIV+ subjects had increased serum levels of neopterin and beta2-microglobulin (both P < 0.0001), probably reflecting immunostimulation. Serum neopterin and beta2-microglobulin were inversely related to duodenal gamma/delta IEL, particularly in the premortal group (r = -0.97 and r = -0.58, respectively). The increased gamma/delta IEL might reflect enhanced intestinal protection in late-phase HIV infection. Short survival expectancy (<7 months) was associated not only with high levels of neopterin and beta2-microglobulin but also with a reduced number of duodenal gamma/delta+ cells (P < 0.03). SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/8648688/Intraepithelial_gamma/delta_T_cells_in_duodenal_mucosa_are_related_to_the_immune_state_and_survival_time_in_AIDS_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&amp;pmid=8648688 DB - PRIME DP - Unbound Medicine ER -