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An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing.
Nature. 1996 Jun 20; 381(6584):709-13.Nat

Abstract

The hydrolysis of ATP by a group of RNA-dependent ATPases (DEAD/H proteins) is required for spliceosome assembly, but not for the subsequent transesterification reactions. Little is known about the function of these ATPases in relation to the RNA conformational changes that occur in formation of active structures, in which U2/U6 small nuclear RNA (snRNA) interactions are essential for splicing to take place. Using a synthetic lethal genetic screen, we have isolated four yeast splicing factors involved in U2/U6 snRNA interactions (D.X. et al., manuscript in preparation). The RNA-dependent ATPase activity associated with one such factor, the Slt22 protein, is stimulated preferentially by annealed U2/U6 snRNAs. Both mutant slt22-1 and U2 snRNA cause a reduction in stimulation. The slt22-1 mutation blocks splicing at or before the first step, resulting in the accumulation of an unusual complex which lacks U5 snRNA. Our results indicate that the U2/U6 snRNA interactions facilitated by Slt22 are also involved in the interaction of U5 snRNA with the spliceosome.

Authors+Show Affiliations

Department of Genetics, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8649518

Citation

Xu, D, et al. "An RNA-dependent ATPase Associated With U2/U6 snRNAs in pre-mRNA Splicing." Nature, vol. 381, no. 6584, 1996, pp. 709-13.
Xu D, Nouraini S, Field D, et al. An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature. 1996;381(6584):709-13.
Xu, D., Nouraini, S., Field, D., Tang, S. J., & Friesen, J. D. (1996). An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. Nature, 381(6584), 709-13.
Xu D, et al. An RNA-dependent ATPase Associated With U2/U6 snRNAs in pre-mRNA Splicing. Nature. 1996 Jun 20;381(6584):709-13. PubMed PMID: 8649518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing. AU - Xu,D, AU - Nouraini,S, AU - Field,D, AU - Tang,S J, AU - Friesen,J D, PY - 1996/6/20/pubmed PY - 1996/6/20/medline PY - 1996/6/20/entrez SP - 709 EP - 13 JF - Nature JO - Nature VL - 381 IS - 6584 N2 - The hydrolysis of ATP by a group of RNA-dependent ATPases (DEAD/H proteins) is required for spliceosome assembly, but not for the subsequent transesterification reactions. Little is known about the function of these ATPases in relation to the RNA conformational changes that occur in formation of active structures, in which U2/U6 small nuclear RNA (snRNA) interactions are essential for splicing to take place. Using a synthetic lethal genetic screen, we have isolated four yeast splicing factors involved in U2/U6 snRNA interactions (D.X. et al., manuscript in preparation). The RNA-dependent ATPase activity associated with one such factor, the Slt22 protein, is stimulated preferentially by annealed U2/U6 snRNAs. Both mutant slt22-1 and U2 snRNA cause a reduction in stimulation. The slt22-1 mutation blocks splicing at or before the first step, resulting in the accumulation of an unusual complex which lacks U5 snRNA. Our results indicate that the U2/U6 snRNA interactions facilitated by Slt22 are also involved in the interaction of U5 snRNA with the spliceosome. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/8649518/An_RNA_dependent_ATPase_associated_with_U2/U6_snRNAs_in_pre_mRNA_splicing_ L2 - https://doi.org/10.1038/381709a0 DB - PRIME DP - Unbound Medicine ER -