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Comparison of fragmentation modes for the structural determination of complex oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry.
Rapid Commun Mass Spectrom. 1995; 9(15):1556-61.RC

Abstract

Fragment ions from underivatized N-linked oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry were obtained by spontaneous fragmentation on a magnetic sector mass spectrometer, by post-source decay (PSD) on a reflectron time-of-flight (TOF) instrument and by collision-induced dissociation on a magnetic sector instrument fitted with an orthagonal-TOF analyser. Spontaneous fragmentation on the magnetic sector instrument produced ions mainly by glycosidic cleavage together with two abundant ions formed by cross-ring cleavage of the reducing-terminal residue. The PSD spectra were similar, the majority of ions being formed by glycosidic cleavage. Internal fragment ions were abundant. High-energy collision-induced dissociation spectra recorded with the orthagonal-TOF analyser, differed considerably from the other types of spectra, particularly in the appearance of major fragment ions produced by cross-ring cleavages of most of the constituent monosaccharide residues. These ions allowed much sequence and branching information to be obtained from the oligosaccharide.

Authors+Show Affiliations

Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8652879

Citation

Harvey, D J., et al. "Comparison of Fragmentation Modes for the Structural Determination of Complex Oligosaccharides Ionized By Matrix-assisted Laser Desorption/ionization Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 9, no. 15, 1995, pp. 1556-61.
Harvey DJ, Naven TJ, Küster B, et al. Comparison of fragmentation modes for the structural determination of complex oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom. 1995;9(15):1556-61.
Harvey, D. J., Naven, T. J., Küster, B., Bateman, R. H., Green, M. R., & Critchley, G. (1995). Comparison of fragmentation modes for the structural determination of complex oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 9(15), 1556-61.
Harvey DJ, et al. Comparison of Fragmentation Modes for the Structural Determination of Complex Oligosaccharides Ionized By Matrix-assisted Laser Desorption/ionization Mass Spectrometry. Rapid Commun Mass Spectrom. 1995;9(15):1556-61. PubMed PMID: 8652879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of fragmentation modes for the structural determination of complex oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry. AU - Harvey,D J, AU - Naven,T J, AU - Küster,B, AU - Bateman,R H, AU - Green,M R, AU - Critchley,G, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 1556 EP - 61 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 9 IS - 15 N2 - Fragment ions from underivatized N-linked oligosaccharides ionized by matrix-assisted laser desorption/ionization mass spectrometry were obtained by spontaneous fragmentation on a magnetic sector mass spectrometer, by post-source decay (PSD) on a reflectron time-of-flight (TOF) instrument and by collision-induced dissociation on a magnetic sector instrument fitted with an orthagonal-TOF analyser. Spontaneous fragmentation on the magnetic sector instrument produced ions mainly by glycosidic cleavage together with two abundant ions formed by cross-ring cleavage of the reducing-terminal residue. The PSD spectra were similar, the majority of ions being formed by glycosidic cleavage. Internal fragment ions were abundant. High-energy collision-induced dissociation spectra recorded with the orthagonal-TOF analyser, differed considerably from the other types of spectra, particularly in the appearance of major fragment ions produced by cross-ring cleavages of most of the constituent monosaccharide residues. These ions allowed much sequence and branching information to be obtained from the oligosaccharide. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/8652879/Comparison_of_fragmentation_modes_for_the_structural_determination_of_complex_oligosaccharides_ionized_by_matrix_assisted_laser_desorption/ionization_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.1290091517 DB - PRIME DP - Unbound Medicine ER -