Tags

Type your tag names separated by a space and hit enter

Immunoreactivity of tenascin-C in dentin matrix in dentinogenesis imperfecta associated with osteogenesis imperfecta.
J Dent Res. 1996 Jan; 75(1):581-7.JD

Abstract

Osteogenesis imperfecta (OI) is a heterogeneous group of heritable connective tissue disorders, assigned to different mutations in type I collagen genes. A variety of structural abnormalities of dentin have been described in dentinogenesis imperfecta (DI) associated with OI. To clarify further the constitution of the dentin matrix in OI, we immunostained frozen and paraffin sections of deciduous teeth from four patients, each from a different family, with two monoclonal antibodies (MAbs) to the matrix glycoprotein tenascin-C (TN-C). One of the MAbs recognizes an epitope common to all TN-C isoforms (BC-4), and the other is specific for a splicing variant (BC-2). Normal teeth, oral mucosa, and skin were analyzed for comparison. Staining patterns with the two MAbs did not differ markedly. Normal dentin matrix and odontoblasts were lacking reactivity, but the pulp stained clearly. TN-C reactivity was present in the dentin matrix of all teeth obtained from two patients with different OI phenotypes and DI, and in one out of three teeth from one patient who also had DI. The reactivity was distributed in layers, but the staining patterns varied from one patient to another and from tooth to tooth. Intratubular staining seen in a tooth from the patient with clinically and histologically normal teeth was comparable with that present in normal deciduous teeth. The variation in TN-C expression suggests that, besides genetic heterogeneity, epigenetic factors could influence the composition of the dentin matrix in OI.

Authors+Show Affiliations

Department of Oral Pathology, University of Helsinki, Finland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8655763

Citation

Lukinmaa, P L., et al. "Immunoreactivity of tenascin-C in Dentin Matrix in Dentinogenesis Imperfecta Associated With Osteogenesis Imperfecta." Journal of Dental Research, vol. 75, no. 1, 1996, pp. 581-7.
Lukinmaa PL, Allemanni G, Waltimo J, et al. Immunoreactivity of tenascin-C in dentin matrix in dentinogenesis imperfecta associated with osteogenesis imperfecta. J Dent Res. 1996;75(1):581-7.
Lukinmaa, P. L., Allemanni, G., Waltimo, J., & Zardi, L. (1996). Immunoreactivity of tenascin-C in dentin matrix in dentinogenesis imperfecta associated with osteogenesis imperfecta. Journal of Dental Research, 75(1), 581-7.
Lukinmaa PL, et al. Immunoreactivity of tenascin-C in Dentin Matrix in Dentinogenesis Imperfecta Associated With Osteogenesis Imperfecta. J Dent Res. 1996;75(1):581-7. PubMed PMID: 8655763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoreactivity of tenascin-C in dentin matrix in dentinogenesis imperfecta associated with osteogenesis imperfecta. AU - Lukinmaa,P L, AU - Allemanni,G, AU - Waltimo,J, AU - Zardi,L, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 581 EP - 7 JF - Journal of dental research JO - J Dent Res VL - 75 IS - 1 N2 - Osteogenesis imperfecta (OI) is a heterogeneous group of heritable connective tissue disorders, assigned to different mutations in type I collagen genes. A variety of structural abnormalities of dentin have been described in dentinogenesis imperfecta (DI) associated with OI. To clarify further the constitution of the dentin matrix in OI, we immunostained frozen and paraffin sections of deciduous teeth from four patients, each from a different family, with two monoclonal antibodies (MAbs) to the matrix glycoprotein tenascin-C (TN-C). One of the MAbs recognizes an epitope common to all TN-C isoforms (BC-4), and the other is specific for a splicing variant (BC-2). Normal teeth, oral mucosa, and skin were analyzed for comparison. Staining patterns with the two MAbs did not differ markedly. Normal dentin matrix and odontoblasts were lacking reactivity, but the pulp stained clearly. TN-C reactivity was present in the dentin matrix of all teeth obtained from two patients with different OI phenotypes and DI, and in one out of three teeth from one patient who also had DI. The reactivity was distributed in layers, but the staining patterns varied from one patient to another and from tooth to tooth. Intratubular staining seen in a tooth from the patient with clinically and histologically normal teeth was comparable with that present in normal deciduous teeth. The variation in TN-C expression suggests that, besides genetic heterogeneity, epigenetic factors could influence the composition of the dentin matrix in OI. SN - 0022-0345 UR - https://www.unboundmedicine.com/medline/citation/8655763/Immunoreactivity_of_tenascin_C_in_dentin_matrix_in_dentinogenesis_imperfecta_associated_with_osteogenesis_imperfecta_ L2 - https://journals.sagepub.com/doi/10.1177/00220345960750011101?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -