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Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction.
Scand J Infect Dis. 1995; 27(4):363-7.SJ

Abstract

To evaluate polymerase chain reaction (PCR) for detection of Pneumocystis carinii, 117 bronchoalveolar lavage (BAL) specimens, from HIV-infected patients undergoing a diagnostic bronchoscopy, were processed and a nested PCR, followed by Southern blot and hybridization with a P32-labelled probe was performed. The sensitivity and specificity were 85 and 100% 934/40 and 77/77) respectively. A non-radioactive labelling system BluGENE was evaluated on all specimens, and found to be as effective as P32-labelling. To increase the speed and convenience of detection, a dot blot system was tested, but sensitivity dropped markedly with this system. A further 33 patients had both induced sputum and bronchoalveolar lavage performed and the induced sputum was analysed using PCR and routine microbiological methods. The PCR sensitivity on induced sputum was equal to that of routine methods. At present the evaluated PCR cannot replace routine microbiological methods for detection of Pneumocystis carinii, on either BAL fluid or induced sputum.

Authors+Show Affiliations

Department of Infectious Diseases, University of Copenhagen, Hvidovre Hospital, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8658071

Citation

Skøt, J, et al. "Pneumocystis Carinii in Bronchoalveolar Lavage and Induced Sputum: Detection With a Nested Polymerase Chain Reaction." Scandinavian Journal of Infectious Diseases, vol. 27, no. 4, 1995, pp. 363-7.
Skøt J, Lerche AG, Kolmos HJ, et al. Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction. Scand J Infect Dis. 1995;27(4):363-7.
Skøt, J., Lerche, A. G., Kolmos, H. J., Nielsen, J. O., Mathiesen, L. R., & Lundgren, J. D. (1995). Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction. Scandinavian Journal of Infectious Diseases, 27(4), 363-7.
Skøt J, et al. Pneumocystis Carinii in Bronchoalveolar Lavage and Induced Sputum: Detection With a Nested Polymerase Chain Reaction. Scand J Infect Dis. 1995;27(4):363-7. PubMed PMID: 8658071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction. AU - Skøt,J, AU - Lerche,A G, AU - Kolmos,H J, AU - Nielsen,J O, AU - Mathiesen,L R, AU - Lundgren,J D, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 363 EP - 7 JF - Scandinavian journal of infectious diseases JO - Scand J Infect Dis VL - 27 IS - 4 N2 - To evaluate polymerase chain reaction (PCR) for detection of Pneumocystis carinii, 117 bronchoalveolar lavage (BAL) specimens, from HIV-infected patients undergoing a diagnostic bronchoscopy, were processed and a nested PCR, followed by Southern blot and hybridization with a P32-labelled probe was performed. The sensitivity and specificity were 85 and 100% 934/40 and 77/77) respectively. A non-radioactive labelling system BluGENE was evaluated on all specimens, and found to be as effective as P32-labelling. To increase the speed and convenience of detection, a dot blot system was tested, but sensitivity dropped markedly with this system. A further 33 patients had both induced sputum and bronchoalveolar lavage performed and the induced sputum was analysed using PCR and routine microbiological methods. The PCR sensitivity on induced sputum was equal to that of routine methods. At present the evaluated PCR cannot replace routine microbiological methods for detection of Pneumocystis carinii, on either BAL fluid or induced sputum. SN - 0036-5548 UR - https://www.unboundmedicine.com/medline/citation/8658071/Pneumocystis_carinii_in_bronchoalveolar_lavage_and_induced_sputum:_detection_with_a_nested_polymerase_chain_reaction_ L2 - https://www.tandfonline.com/doi/full/10.3109/00365549509032732 DB - PRIME DP - Unbound Medicine ER -