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IFN-gamma-stimulated enhancement of MHC class II antigen expression by the human mast cell line HMC-1.
Cell Immunol. 1996 May 25; 170(1):85-90.CI

Abstract

The expression of MHC class II molecules by human mast cells has been reported in immunohistochemical surveys of inflammatory conditions, such as in tuberculin hypersensitivity. While these data suggest that human mast cells may act as antigen-presenting cells under inflammatory conditions, the induction of class II antigens on human mast cells has not been examined. In this study, we determined the effects of the inflammatory cytokines IFN-gamma and IL-4 on the expression of class II antigens HLA-DR, -DP, and -DQ by the human mast cell line HMC-1. HMC-1 cells were incubated with or without 1000 U/ml recombinant human IFN-gamma (rhIFN-gamma) and IL-4 (rhIL-4) for 72 hr and analyzed for expression of MHC class II antigens by direct immunofluorescence and flow cytometry. HMC-1 cells expressed significant levels of HLA-DR and moderate levels of HLA-DP and -DQ at baseline and when cultured without exogenous cytokines. Stimulation by rhIFN-gamma for 72 hr significantly increased the levels of HLA-DR and -DP expression but did not affect levels of HLA-DQ. Stimulation by rhIL-4 for 72 hr had minimal effect on expression of class II molecules, but induced a significant difference in levels of ICAM-1 (CD54) expression, indicating that this cytokine is involved instead in the control of certain accessory molecules. Our data showing constitutive expression of MHC class II molecules on HMC-1 cells and upregulation of that expression by rhIFN-gamma suggest that human mast cells function as antigen-presenting cells at sites where inflammatory cytokines are present.

Authors+Show Affiliations

Department of Medical Microbiology, Ohio State University College of Medicine, Columbus, 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8660803

Citation

Love, K S., et al. "IFN-gamma-stimulated Enhancement of MHC Class II Antigen Expression By the Human Mast Cell Line HMC-1." Cellular Immunology, vol. 170, no. 1, 1996, pp. 85-90.
Love KS, Lakshmanan RR, Butterfield JH, et al. IFN-gamma-stimulated enhancement of MHC class II antigen expression by the human mast cell line HMC-1. Cell Immunol. 1996;170(1):85-90.
Love, K. S., Lakshmanan, R. R., Butterfield, J. H., & Fox, C. C. (1996). IFN-gamma-stimulated enhancement of MHC class II antigen expression by the human mast cell line HMC-1. Cellular Immunology, 170(1), 85-90.
Love KS, et al. IFN-gamma-stimulated Enhancement of MHC Class II Antigen Expression By the Human Mast Cell Line HMC-1. Cell Immunol. 1996 May 25;170(1):85-90. PubMed PMID: 8660803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IFN-gamma-stimulated enhancement of MHC class II antigen expression by the human mast cell line HMC-1. AU - Love,K S, AU - Lakshmanan,R R, AU - Butterfield,J H, AU - Fox,C C, PY - 1996/5/25/pubmed PY - 1996/5/25/medline PY - 1996/5/25/entrez SP - 85 EP - 90 JF - Cellular immunology JO - Cell Immunol VL - 170 IS - 1 N2 - The expression of MHC class II molecules by human mast cells has been reported in immunohistochemical surveys of inflammatory conditions, such as in tuberculin hypersensitivity. While these data suggest that human mast cells may act as antigen-presenting cells under inflammatory conditions, the induction of class II antigens on human mast cells has not been examined. In this study, we determined the effects of the inflammatory cytokines IFN-gamma and IL-4 on the expression of class II antigens HLA-DR, -DP, and -DQ by the human mast cell line HMC-1. HMC-1 cells were incubated with or without 1000 U/ml recombinant human IFN-gamma (rhIFN-gamma) and IL-4 (rhIL-4) for 72 hr and analyzed for expression of MHC class II antigens by direct immunofluorescence and flow cytometry. HMC-1 cells expressed significant levels of HLA-DR and moderate levels of HLA-DP and -DQ at baseline and when cultured without exogenous cytokines. Stimulation by rhIFN-gamma for 72 hr significantly increased the levels of HLA-DR and -DP expression but did not affect levels of HLA-DQ. Stimulation by rhIL-4 for 72 hr had minimal effect on expression of class II molecules, but induced a significant difference in levels of ICAM-1 (CD54) expression, indicating that this cytokine is involved instead in the control of certain accessory molecules. Our data showing constitutive expression of MHC class II molecules on HMC-1 cells and upregulation of that expression by rhIFN-gamma suggest that human mast cells function as antigen-presenting cells at sites where inflammatory cytokines are present. SN - 0008-8749 UR - https://www.unboundmedicine.com/medline/citation/8660803/IFN_gamma_stimulated_enhancement_of_MHC_class_II_antigen_expression_by_the_human_mast_cell_line_HMC_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-8749(96)90137-9 DB - PRIME DP - Unbound Medicine ER -