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Identification of cis-acting DNA sequences involved in the transcription of the virulence regulatory gene spvR in Salmonella typhimurium.
Mol Gen Genet. 1996 May 23; 251(2):225-35.MG

Abstract

The SpvR protein is a DNA-binding protein of the LysR family, required for the transcription of the spvABCD virulence operon of Salmonella typhimurium. An alternative sigma factor, sigma S (RpoS), in conjunction with SpvR, controls the transcription of the spvR gene. In this study, we used a combination of primer extension experiments and deletion/fusion analyses of the spvR gene to identify sequences involved in spvR transcription in S. typhimurium. When induced in the stationary phase of growth in rich medium or during carbon starvation, transcription of spvR in S. typhimurium is driven by a single promoter (spvRp1) and initiates 17 nucleotides upstream of the spvR start codon. The level of spvR transcription originating at spvRp1 was 20-fold higher in the wild-type strain than in the rpoS mutant. In both strains, however, transcription at spvRp1 requires the SpvR protein. 5' Deletions up to position -86, relative to the spvR start codon, did not inhibit inducibility by sigma S and/or SPVR. In contrast, 5' deletion up to -75 abolished the activation of spvRp1 by SpvR in both the wild-type strain and rpoS mutant. Within the 11-bp sequence lying between position -86 and position -75, a 10-bp consensus motif TNTNTGCANA, present in both the spvR and spvA promoter regions, was identified and may contain the DNA recognition site for SpvR. In addition, we detected initiation of transcription within the spvR coding region. This finding may have implications for comparative studies of regulation with spvR gene fusions.

Authors+Show Affiliations

Institut Pasteur, Unité des Entérobactéries, INSERM U389, Paris, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8668134

Citation

Kowarz, L, et al. "Identification of Cis-acting DNA Sequences Involved in the Transcription of the Virulence Regulatory Gene spvR in Salmonella Typhimurium." Molecular & General Genetics : MGG, vol. 251, no. 2, 1996, pp. 225-35.
Kowarz L, Robbe-Saule V, Norel F. Identification of cis-acting DNA sequences involved in the transcription of the virulence regulatory gene spvR in Salmonella typhimurium. Mol Gen Genet. 1996;251(2):225-35.
Kowarz, L., Robbe-Saule, V., & Norel, F. (1996). Identification of cis-acting DNA sequences involved in the transcription of the virulence regulatory gene spvR in Salmonella typhimurium. Molecular & General Genetics : MGG, 251(2), 225-35.
Kowarz L, Robbe-Saule V, Norel F. Identification of Cis-acting DNA Sequences Involved in the Transcription of the Virulence Regulatory Gene spvR in Salmonella Typhimurium. Mol Gen Genet. 1996 May 23;251(2):225-35. PubMed PMID: 8668134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of cis-acting DNA sequences involved in the transcription of the virulence regulatory gene spvR in Salmonella typhimurium. AU - Kowarz,L, AU - Robbe-Saule,V, AU - Norel,F, PY - 1996/5/23/pubmed PY - 1996/5/23/medline PY - 1996/5/23/entrez SP - 225 EP - 35 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 251 IS - 2 N2 - The SpvR protein is a DNA-binding protein of the LysR family, required for the transcription of the spvABCD virulence operon of Salmonella typhimurium. An alternative sigma factor, sigma S (RpoS), in conjunction with SpvR, controls the transcription of the spvR gene. In this study, we used a combination of primer extension experiments and deletion/fusion analyses of the spvR gene to identify sequences involved in spvR transcription in S. typhimurium. When induced in the stationary phase of growth in rich medium or during carbon starvation, transcription of spvR in S. typhimurium is driven by a single promoter (spvRp1) and initiates 17 nucleotides upstream of the spvR start codon. The level of spvR transcription originating at spvRp1 was 20-fold higher in the wild-type strain than in the rpoS mutant. In both strains, however, transcription at spvRp1 requires the SpvR protein. 5' Deletions up to position -86, relative to the spvR start codon, did not inhibit inducibility by sigma S and/or SPVR. In contrast, 5' deletion up to -75 abolished the activation of spvRp1 by SpvR in both the wild-type strain and rpoS mutant. Within the 11-bp sequence lying between position -86 and position -75, a 10-bp consensus motif TNTNTGCANA, present in both the spvR and spvA promoter regions, was identified and may contain the DNA recognition site for SpvR. In addition, we detected initiation of transcription within the spvR coding region. This finding may have implications for comparative studies of regulation with spvR gene fusions. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/8668134/Identification_of_cis_acting_DNA_sequences_involved_in_the_transcription_of_the_virulence_regulatory_gene_spvR_in_Salmonella_typhimurium_ L2 - https://doi.org/10.1007/BF02172922 DB - PRIME DP - Unbound Medicine ER -