Tags

Type your tag names separated by a space and hit enter

Direct amplification and species determination of microsporidian DNA from stool specimens.
Trop Med Int Health. 1996 Jun; 1(3):373-8.TM

Abstract

Microsporidia are recognized as a major aetiological agent in chronic diarrhoea of immunocompromised patients. Their detection by light microscopy is hampered by the small size of the spores. A simple and rapid DNA extraction method has been developed for the detection of microsporidian DNA by PCR directly from stool specimens. It can be performed at room temperature in a 1.5-ml microcentrifuge tube format in less than 1 hour. The subsequent nested polymerase chain reaction permits the detection of 3-100 spores in a 0.1-g stool sample. The amplification products can be verified and the species Enterocytozoon bieneusi, Encephalitozoon cuniculi and Encephalitozoon (Septata) intestinalis distinguished by a simple restriction endonuclease digest.

Authors+Show Affiliations

Department of Infectious Diseases and Tropical Medicine, University of Munich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8673842

Citation

Katzwinkel-Wladarsch, S, et al. "Direct Amplification and Species Determination of Microsporidian DNA From Stool Specimens." Tropical Medicine & International Health : TM & IH, vol. 1, no. 3, 1996, pp. 373-8.
Katzwinkel-Wladarsch S, Lieb M, Helse W, et al. Direct amplification and species determination of microsporidian DNA from stool specimens. Trop Med Int Health. 1996;1(3):373-8.
Katzwinkel-Wladarsch, S., Lieb, M., Helse, W., Löscher, T., & Rinder, H. (1996). Direct amplification and species determination of microsporidian DNA from stool specimens. Tropical Medicine & International Health : TM & IH, 1(3), 373-8.
Katzwinkel-Wladarsch S, et al. Direct Amplification and Species Determination of Microsporidian DNA From Stool Specimens. Trop Med Int Health. 1996;1(3):373-8. PubMed PMID: 8673842.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct amplification and species determination of microsporidian DNA from stool specimens. AU - Katzwinkel-Wladarsch,S, AU - Lieb,M, AU - Helse,W, AU - Löscher,T, AU - Rinder,H, PY - 1996/6/1/pubmed PY - 1996/6/1/medline PY - 1996/6/1/entrez SP - 373 EP - 8 JF - Tropical medicine & international health : TM & IH JO - Trop Med Int Health VL - 1 IS - 3 N2 - Microsporidia are recognized as a major aetiological agent in chronic diarrhoea of immunocompromised patients. Their detection by light microscopy is hampered by the small size of the spores. A simple and rapid DNA extraction method has been developed for the detection of microsporidian DNA by PCR directly from stool specimens. It can be performed at room temperature in a 1.5-ml microcentrifuge tube format in less than 1 hour. The subsequent nested polymerase chain reaction permits the detection of 3-100 spores in a 0.1-g stool sample. The amplification products can be verified and the species Enterocytozoon bieneusi, Encephalitozoon cuniculi and Encephalitozoon (Septata) intestinalis distinguished by a simple restriction endonuclease digest. SN - 1360-2276 UR - https://www.unboundmedicine.com/medline/citation/8673842/Direct_amplification_and_species_determination_of_microsporidian_DNA_from_stool_specimens_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1360-2276&date=1996&volume=1&issue=3&spage=373 DB - PRIME DP - Unbound Medicine ER -