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hnRNP A1 selectively interacts through its Gly-rich domain with different RNA-binding proteins.
J Mol Biol. 1996 Jun 14; 259(3):337-48.JM

Abstract

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are abundant nuclear polypeptides, most likely involved in different steps of pre-mRNA processing. Protein A1 (34 kDa), a prominent member of the hnRNP family, seems to act by modulating the RNA secondary structure and by antagonizing some splicing factors (SR proteins) in splice-site selection and exon skipping/inclusion. A role of A1 in the nucleo-cytoplasmic transport of RNA has also been proposed. These activities might depend not only on the RNA-binding properties of the protein but also on specific protein-protein interactions. Here we report that A1 can indeed selectively interact, in vitro, both with itself and with other hnRNP basic "core" proteins. Such selective binding is mediated exclusively by the Gly-rich C-terminal domain, where a novel protein-binding motif constituted by hydrophobic repeats can be envisaged. The same domain is necessary and sufficient to promote specific interaction in vivo, as assayed by the yeast two-hybrid assay. Moreover, an in vitro interaction with some SR proteins was also observed. These observations suggest that diverse and specific protein-protein interactions might contribute to the different functions of the hnRNP A1 protein in mRNA maturation.

Authors+Show Affiliations

Istituto di Genetica Biochimica ed Evoluzionistica C.N.R., Pavia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8676373

Citation

Cartegni, L, et al. "HnRNP A1 Selectively Interacts Through Its Gly-rich Domain With Different RNA-binding Proteins." Journal of Molecular Biology, vol. 259, no. 3, 1996, pp. 337-48.
Cartegni L, Maconi M, Morandi E, et al. HnRNP A1 selectively interacts through its Gly-rich domain with different RNA-binding proteins. J Mol Biol. 1996;259(3):337-48.
Cartegni, L., Maconi, M., Morandi, E., Cobianchi, F., Riva, S., & Biamonti, G. (1996). HnRNP A1 selectively interacts through its Gly-rich domain with different RNA-binding proteins. Journal of Molecular Biology, 259(3), 337-48.
Cartegni L, et al. HnRNP A1 Selectively Interacts Through Its Gly-rich Domain With Different RNA-binding Proteins. J Mol Biol. 1996 Jun 14;259(3):337-48. PubMed PMID: 8676373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - hnRNP A1 selectively interacts through its Gly-rich domain with different RNA-binding proteins. AU - Cartegni,L, AU - Maconi,M, AU - Morandi,E, AU - Cobianchi,F, AU - Riva,S, AU - Biamonti,G, PY - 1996/6/14/pubmed PY - 1996/6/14/medline PY - 1996/6/14/entrez SP - 337 EP - 48 JF - Journal of molecular biology JO - J Mol Biol VL - 259 IS - 3 N2 - Heterogeneous nuclear ribonucleoproteins (hnRNPs) are abundant nuclear polypeptides, most likely involved in different steps of pre-mRNA processing. Protein A1 (34 kDa), a prominent member of the hnRNP family, seems to act by modulating the RNA secondary structure and by antagonizing some splicing factors (SR proteins) in splice-site selection and exon skipping/inclusion. A role of A1 in the nucleo-cytoplasmic transport of RNA has also been proposed. These activities might depend not only on the RNA-binding properties of the protein but also on specific protein-protein interactions. Here we report that A1 can indeed selectively interact, in vitro, both with itself and with other hnRNP basic "core" proteins. Such selective binding is mediated exclusively by the Gly-rich C-terminal domain, where a novel protein-binding motif constituted by hydrophobic repeats can be envisaged. The same domain is necessary and sufficient to promote specific interaction in vivo, as assayed by the yeast two-hybrid assay. Moreover, an in vitro interaction with some SR proteins was also observed. These observations suggest that diverse and specific protein-protein interactions might contribute to the different functions of the hnRNP A1 protein in mRNA maturation. SN - 0022-2836 UR - https://www.unboundmedicine.com/medline/citation/8676373/hnRNP_A1_selectively_interacts_through_its_Gly_rich_domain_with_different_RNA_binding_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2836(96)90324-1 DB - PRIME DP - Unbound Medicine ER -