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Separation of amino acid enantiomers by micellar electrokinetic capillary chromatography using synthetic chiral surfactants.
Anal Biochem. 1995 Oct 10; 231(1):65-71.AB

Abstract

A synthetic chiral surfactant was employed for the enantioselective micellar electrokinetic capillary chromatographic (MECC) separation of amino acid enantiomers derivatized with 6-aminoquinoyl-N-hydroxysuccinimidyl carbamate (AQC). The effect of surfactant concentration and buffer pH on resolution was studied, and the optimized conditions were used to evaluate the method in terms of sensitivity, reproducibility, and linearity. Resolution and alpha values for 12 ACQ-derivatized amino acids are reported. The ability to perform both achiral and chiral separations simultaneously is illustrated in a separation of a mixture of six amino acid enantiomeric pairs, all with baseline resolution. The direct reversal of enantiomer migration order, useful in quantitation and chiral identification, is also shown. Comparisons with other N-protected amino acid derivatives are made in terms of resolution and sensitivity, and the advantages of this chiral MECC technique used in conjunction with the inherent advantages of the AQC derivatizing reagent are discussed.

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Authors+Show Affiliations

Swartz ME
Waters Corporation, Milford, Massachusetts 01757, USA.
Mazzeo JR
No affiliation info available
Grover ER
No affiliation info available
Brown PR
No affiliation info available

MeSH

Amino AcidsChromatographyElectrophoresis, CapillaryStereoisomerismSurface-Active Agents

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8678322

Citation

Swartz, M E., et al. "Separation of Amino Acid Enantiomers By Micellar Electrokinetic Capillary Chromatography Using Synthetic Chiral Surfactants." Analytical Biochemistry, vol. 231, no. 1, 1995, pp. 65-71.
Swartz ME, Mazzeo JR, Grover ER, et al. Separation of amino acid enantiomers by micellar electrokinetic capillary chromatography using synthetic chiral surfactants. Anal Biochem. 1995;231(1):65-71.
Swartz, M. E., Mazzeo, J. R., Grover, E. R., & Brown, P. R. (1995). Separation of amino acid enantiomers by micellar electrokinetic capillary chromatography using synthetic chiral surfactants. Analytical Biochemistry, 231(1), 65-71.
Swartz ME, et al. Separation of Amino Acid Enantiomers By Micellar Electrokinetic Capillary Chromatography Using Synthetic Chiral Surfactants. Anal Biochem. 1995 Oct 10;231(1):65-71. PubMed PMID: 8678322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of amino acid enantiomers by micellar electrokinetic capillary chromatography using synthetic chiral surfactants. AU - Swartz,M E, AU - Mazzeo,J R, AU - Grover,E R, AU - Brown,P R, PY - 1995/10/10/pubmed PY - 1995/10/10/medline PY - 1995/10/10/entrez SP - 65 EP - 71 JF - Analytical biochemistry JO - Anal Biochem VL - 231 IS - 1 N2 - A synthetic chiral surfactant was employed for the enantioselective micellar electrokinetic capillary chromatographic (MECC) separation of amino acid enantiomers derivatized with 6-aminoquinoyl-N-hydroxysuccinimidyl carbamate (AQC). The effect of surfactant concentration and buffer pH on resolution was studied, and the optimized conditions were used to evaluate the method in terms of sensitivity, reproducibility, and linearity. Resolution and alpha values for 12 ACQ-derivatized amino acids are reported. The ability to perform both achiral and chiral separations simultaneously is illustrated in a separation of a mixture of six amino acid enantiomeric pairs, all with baseline resolution. The direct reversal of enantiomer migration order, useful in quantitation and chiral identification, is also shown. Comparisons with other N-protected amino acid derivatives are made in terms of resolution and sensitivity, and the advantages of this chiral MECC technique used in conjunction with the inherent advantages of the AQC derivatizing reagent are discussed. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/8678322/Separation_of_amino_acid_enantiomers_by_micellar_electrokinetic_capillary_chromatography_using_synthetic_chiral_surfactants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(85)71504-7 DB - PRIME DP - Unbound Medicine ER -