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Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli.
Chin J Biotechnol. 1995; 11(3):185-91.CJ

Abstract

Thermostable DNA polymerase genes had been amplified from Thermus aquaticus YT-1 using the PCR technique. The amplified 2.5-kb DNA fragment was inserted into pUC18 and confirmed to be a thermostable DNA polymerase gene by restriction mapping and DNA sequencing. The insert fragment was then combined into an expression vector, pBV221. This recombinant plasmid overexpressed a 94-kDa of recombinant protein in E. coli. A 100-mL E. coli. culture could yield 1.5 x 10(5) units of Taq DNA polymerase, which could be applied in the PCR.

Authors+Show Affiliations

Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8679935

Citation

Jin, C, et al. "Cloning and Overexpression of Thermostable DNA Polymerase Gene in Escherichia Coli." Chinese Journal of Biotechnology, vol. 11, no. 3, 1995, pp. 185-91.
Jin C, Liu H, Yang S, et al. Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli. Chin J Biotechnol. 1995;11(3):185-91.
Jin, C., Liu, H., Yang, S., & Zhang, S. (1995). Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli. Chinese Journal of Biotechnology, 11(3), 185-91.
Jin C, et al. Cloning and Overexpression of Thermostable DNA Polymerase Gene in Escherichia Coli. Chin J Biotechnol. 1995;11(3):185-91. PubMed PMID: 8679935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and overexpression of thermostable DNA polymerase gene in Escherichia coli. AU - Jin,C, AU - Liu,H, AU - Yang,S, AU - Zhang,S, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 185 EP - 91 JF - Chinese journal of biotechnology JO - Chin J Biotechnol VL - 11 IS - 3 N2 - Thermostable DNA polymerase genes had been amplified from Thermus aquaticus YT-1 using the PCR technique. The amplified 2.5-kb DNA fragment was inserted into pUC18 and confirmed to be a thermostable DNA polymerase gene by restriction mapping and DNA sequencing. The insert fragment was then combined into an expression vector, pBV221. This recombinant plasmid overexpressed a 94-kDa of recombinant protein in E. coli. A 100-mL E. coli. culture could yield 1.5 x 10(5) units of Taq DNA polymerase, which could be applied in the PCR. SN - 1042-749X UR - https://www.unboundmedicine.com/medline/citation/8679935/Cloning_and_overexpression_of_thermostable_DNA_polymerase_gene_in_Escherichia_coli_ DB - PRIME DP - Unbound Medicine ER -