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Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene.
J Bacteriol. 1996 Jul; 178(13):3763-70.JB

Abstract

The RpoS transcription factor (also called sigma Sor sigma 38) is required for the expression of a number of stationary-phase and osmotically inducible genes in Escherichia coli. RpoS is also a virulence factor for several pathogenic bacteria, including Salmonella typhimurium. The activity of RpoS is regulated in response to several different signals, at the transcriptional and translational levels as well as by proteolysis. Here we report that host factor I (HF-I), the product of the hfq gene, is required for efficient expression of rpoS in S. typhimurium. HF-I is a small, heat-stable, site-specific RNA-binding protein originally characterized for its role in replication of the RNA bacteriophage Q beta of E. coli. Its role in the uninfected bacterial cell has previously been unknown. Assays of Beta-galactosidase in strains with rpoS-lac fusions, Western blot (immunoblot) analysis, and pulse-labeling and immunoprecipitation of both fusion proteins and native RpoS show that an S. typhimurium hfq mutant has a four- to sevenfold reduction in expression of rpoS that is attributable primarily to a defect in translation. These results add a new level of complexity to the regulation of RpoS activity.

Authors+Show Affiliations

Department of Microbiology and Immunology, West Virginia University Health Sciences Center, Morgantown, West Virginia 26506, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8682778

Citation

Brown, L, and T Elliott. "Efficient Translation of the RpoS Sigma Factor in Salmonella Typhimurium Requires Host Factor I, an RNA-binding Protein Encoded By the Hfq Gene." Journal of Bacteriology, vol. 178, no. 13, 1996, pp. 3763-70.
Brown L, Elliott T. Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene. J Bacteriol. 1996;178(13):3763-70.
Brown, L., & Elliott, T. (1996). Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene. Journal of Bacteriology, 178(13), 3763-70.
Brown L, Elliott T. Efficient Translation of the RpoS Sigma Factor in Salmonella Typhimurium Requires Host Factor I, an RNA-binding Protein Encoded By the Hfq Gene. J Bacteriol. 1996;178(13):3763-70. PubMed PMID: 8682778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene. AU - Brown,L, AU - Elliott,T, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - 3763 EP - 70 JF - Journal of bacteriology JO - J Bacteriol VL - 178 IS - 13 N2 - The RpoS transcription factor (also called sigma Sor sigma 38) is required for the expression of a number of stationary-phase and osmotically inducible genes in Escherichia coli. RpoS is also a virulence factor for several pathogenic bacteria, including Salmonella typhimurium. The activity of RpoS is regulated in response to several different signals, at the transcriptional and translational levels as well as by proteolysis. Here we report that host factor I (HF-I), the product of the hfq gene, is required for efficient expression of rpoS in S. typhimurium. HF-I is a small, heat-stable, site-specific RNA-binding protein originally characterized for its role in replication of the RNA bacteriophage Q beta of E. coli. Its role in the uninfected bacterial cell has previously been unknown. Assays of Beta-galactosidase in strains with rpoS-lac fusions, Western blot (immunoblot) analysis, and pulse-labeling and immunoprecipitation of both fusion proteins and native RpoS show that an S. typhimurium hfq mutant has a four- to sevenfold reduction in expression of rpoS that is attributable primarily to a defect in translation. These results add a new level of complexity to the regulation of RpoS activity. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8682778/Efficient_translation_of_the_RpoS_sigma_factor_in_Salmonella_typhimurium_requires_host_factor_I_an_RNA_binding_protein_encoded_by_the_hfq_gene_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8682778 DB - PRIME DP - Unbound Medicine ER -