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Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish.
Nature. 1996 Aug 01; 382(6590):452-5.Nat

Abstract

The notochord plays a central role in vertebrate development, acting as a signalling source that patterns the neural tube and somites. In in vitro assays, the secreted protein Sonic hedgehog mimics the inducing effects of notochord on both presomitic mesoderm and neural plate explants of amniote embryos, suggesting that both patterning activities of the notochord may be mediated by this protein in vivo. In zebrafish, however, mutants with disrupted notochord development lack a specific muscle cell type, the muscle pioneers, although they retain the ability to induce neural differentiation, raising the possibility that neural tube and somite patterning may be mediated by distinct signals. Here we describe a new member of the hedgehog family, echidna hedgehog, that is expressed exclusively in the notochord and has the ability to rescue the differentiation of muscle pioneer cells in mutants with no notochord. Moreover, we show that a combination of ectopic echidna hedgehog and sonic hedgehog expression induces supernumary muscle pioneers in wild-type embryos, suggesting that both signals act sequentially to pattern the developing somites.

Authors+Show Affiliations

Molecular Embryology Laboratory, Imperial Cancer Research Fund, London, UK.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8684485

Citation

Currie, P D., and P W. Ingham. "Induction of a Specific Muscle Cell Type By a Hedgehog-like Protein in Zebrafish." Nature, vol. 382, no. 6590, 1996, pp. 452-5.
Currie PD, Ingham PW. Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish. Nature. 1996;382(6590):452-5.
Currie, P. D., & Ingham, P. W. (1996). Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish. Nature, 382(6590), 452-5.
Currie PD, Ingham PW. Induction of a Specific Muscle Cell Type By a Hedgehog-like Protein in Zebrafish. Nature. 1996 Aug 1;382(6590):452-5. PubMed PMID: 8684485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of a specific muscle cell type by a hedgehog-like protein in zebrafish. AU - Currie,P D, AU - Ingham,P W, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 452 EP - 5 JF - Nature JO - Nature VL - 382 IS - 6590 N2 - The notochord plays a central role in vertebrate development, acting as a signalling source that patterns the neural tube and somites. In in vitro assays, the secreted protein Sonic hedgehog mimics the inducing effects of notochord on both presomitic mesoderm and neural plate explants of amniote embryos, suggesting that both patterning activities of the notochord may be mediated by this protein in vivo. In zebrafish, however, mutants with disrupted notochord development lack a specific muscle cell type, the muscle pioneers, although they retain the ability to induce neural differentiation, raising the possibility that neural tube and somite patterning may be mediated by distinct signals. Here we describe a new member of the hedgehog family, echidna hedgehog, that is expressed exclusively in the notochord and has the ability to rescue the differentiation of muscle pioneer cells in mutants with no notochord. Moreover, we show that a combination of ectopic echidna hedgehog and sonic hedgehog expression induces supernumary muscle pioneers in wild-type embryos, suggesting that both signals act sequentially to pattern the developing somites. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/8684485/Induction_of_a_specific_muscle_cell_type_by_a_hedgehog_like_protein_in_zebrafish_ L2 - https://doi.org/10.1038/382452a0 DB - PRIME DP - Unbound Medicine ER -