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Basophil histamine release and leukotriene production in response to anti-IgE and anti-IgE receptor antibodies. Comparison of normal subjects and patients with urticaria, atopic dermatitis or bronchial asthma.
Int Arch Allergy Immunol. 1996 Jul; 110(3):261-71.IA

Abstract

The IgE receptor-dependent in vitro mediator release in basophils is characterized by a large interindividual variability both in normal and atopic subjects. The mechanism and the clinical impact of this finding, however, is largely unclear. The aim of the present study was to examine the role of surface-bound IgE and of response-modifying cytokines such as interleukin 3 (IL-3) as possible factors determining basophil releasability in atopic patients and normal controls. Cells from 30 individuals (6 with urticaria, 7 with asthma, 7 with atopic dermatitis, and 30 healthy controls) were isolated and stimulated for mediator release by IL-3 and different triggering antibodies directed against IgE or IgE receptor. Our data suggest that serum IgE levels and basophil receptor occupancy with IgE are not involved in the mechanism of basophil releasability. Furthermore, IL-3-induced similar effects on mediator release in almost all individuals, rather excluding the possibility that releasability is regulated by cytokine priming of basophils. Interestingly, we found that patients with atopic disease have a reduced capacity of releasing mediators upon activation, the mechanism of which is unclear. In conclusion, our findings support the hypothesis that basophil releasability is dependent on cell-imminent mechanisms in basophils, which may be altered in selected atopic patients.

Authors+Show Affiliations

Department of Gastroenterology and Hepatology, Medical School Hannover, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8688673

Citation

Bischoff, S C., et al. "Basophil Histamine Release and Leukotriene Production in Response to anti-IgE and anti-IgE Receptor Antibodies. Comparison of Normal Subjects and Patients With Urticaria, Atopic Dermatitis or Bronchial Asthma." International Archives of Allergy and Immunology, vol. 110, no. 3, 1996, pp. 261-71.
Bischoff SC, Zwahlen R, Stucki M, et al. Basophil histamine release and leukotriene production in response to anti-IgE and anti-IgE receptor antibodies. Comparison of normal subjects and patients with urticaria, atopic dermatitis or bronchial asthma. Int Arch Allergy Immunol. 1996;110(3):261-71.
Bischoff, S. C., Zwahlen, R., Stucki, M., Müllner, G., de Weck, A. L., Stadler, B. M., & Dahinden, C. A. (1996). Basophil histamine release and leukotriene production in response to anti-IgE and anti-IgE receptor antibodies. Comparison of normal subjects and patients with urticaria, atopic dermatitis or bronchial asthma. International Archives of Allergy and Immunology, 110(3), 261-71.
Bischoff SC, et al. Basophil Histamine Release and Leukotriene Production in Response to anti-IgE and anti-IgE Receptor Antibodies. Comparison of Normal Subjects and Patients With Urticaria, Atopic Dermatitis or Bronchial Asthma. Int Arch Allergy Immunol. 1996;110(3):261-71. PubMed PMID: 8688673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Basophil histamine release and leukotriene production in response to anti-IgE and anti-IgE receptor antibodies. Comparison of normal subjects and patients with urticaria, atopic dermatitis or bronchial asthma. AU - Bischoff,S C, AU - Zwahlen,R, AU - Stucki,M, AU - Müllner,G, AU - de Weck,A L, AU - Stadler,B M, AU - Dahinden,C A, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - 261 EP - 71 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 110 IS - 3 N2 - The IgE receptor-dependent in vitro mediator release in basophils is characterized by a large interindividual variability both in normal and atopic subjects. The mechanism and the clinical impact of this finding, however, is largely unclear. The aim of the present study was to examine the role of surface-bound IgE and of response-modifying cytokines such as interleukin 3 (IL-3) as possible factors determining basophil releasability in atopic patients and normal controls. Cells from 30 individuals (6 with urticaria, 7 with asthma, 7 with atopic dermatitis, and 30 healthy controls) were isolated and stimulated for mediator release by IL-3 and different triggering antibodies directed against IgE or IgE receptor. Our data suggest that serum IgE levels and basophil receptor occupancy with IgE are not involved in the mechanism of basophil releasability. Furthermore, IL-3-induced similar effects on mediator release in almost all individuals, rather excluding the possibility that releasability is regulated by cytokine priming of basophils. Interestingly, we found that patients with atopic disease have a reduced capacity of releasing mediators upon activation, the mechanism of which is unclear. In conclusion, our findings support the hypothesis that basophil releasability is dependent on cell-imminent mechanisms in basophils, which may be altered in selected atopic patients. SN - 1018-2438 UR - https://www.unboundmedicine.com/medline/citation/8688673/Basophil_histamine_release_and_leukotriene_production_in_response_to_anti_IgE_and_anti_IgE_receptor_antibodies__Comparison_of_normal_subjects_and_patients_with_urticaria_atopic_dermatitis_or_bronchial_asthma_ L2 - https://www.karger.com?DOI=10.1159/000237297 DB - PRIME DP - Unbound Medicine ER -