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Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication.
Proc Natl Acad Sci U S A. 1996 Jul 09; 93(14):7131-6.PN

Abstract

In wild-type diploid cells of Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) at the MAT locus can be efficiently repaired by gene conversion using the homologous chromosome sequences. Repair of the broken chromosome was nearly eliminated in rad52delta diploids; 99% lost the broken chromosome. However, in rad51delta diploids, the broken chromosomes were repaired approximately 35% of the time. None of these repair events were simple gene conversions or gene conversions with an associated crossover, instead, they created diploids homozygous for the MAT locus and all markers in the 100-kb region distal to the site of the DSB. In rad51delta diploids, the broken chromosome can apparently be inherited for several generations, as many of these repair events are found as sectored colonies, with one part being repaired and the other part being lost the broken chromosome. Similar events occur in about 2% of wild-type cells. We propose that a broken chromosome end can invade a homologous template in the absence of RAD51 and initiate DNA replication that may extend to the telomere, 100 or more kb away. Such break-induced replication appears to be similar to recombination-initiated replication in bacteria.

Authors+Show Affiliations

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02254-9110, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8692957

Citation

Malkova, A, et al. "Double-strand Break Repair in the Absence of RAD51 in Yeast: a Possible Role for Break-induced DNA Replication." Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 14, 1996, pp. 7131-6.
Malkova A, Ivanov EL, Haber JE. Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. Proc Natl Acad Sci U S A. 1996;93(14):7131-6.
Malkova, A., Ivanov, E. L., & Haber, J. E. (1996). Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. Proceedings of the National Academy of Sciences of the United States of America, 93(14), 7131-6.
Malkova A, Ivanov EL, Haber JE. Double-strand Break Repair in the Absence of RAD51 in Yeast: a Possible Role for Break-induced DNA Replication. Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7131-6. PubMed PMID: 8692957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. AU - Malkova,A, AU - Ivanov,E L, AU - Haber,J E, PY - 1996/7/9/pubmed PY - 1996/7/9/medline PY - 1996/7/9/entrez SP - 7131 EP - 6 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 93 IS - 14 N2 - In wild-type diploid cells of Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) at the MAT locus can be efficiently repaired by gene conversion using the homologous chromosome sequences. Repair of the broken chromosome was nearly eliminated in rad52delta diploids; 99% lost the broken chromosome. However, in rad51delta diploids, the broken chromosomes were repaired approximately 35% of the time. None of these repair events were simple gene conversions or gene conversions with an associated crossover, instead, they created diploids homozygous for the MAT locus and all markers in the 100-kb region distal to the site of the DSB. In rad51delta diploids, the broken chromosome can apparently be inherited for several generations, as many of these repair events are found as sectored colonies, with one part being repaired and the other part being lost the broken chromosome. Similar events occur in about 2% of wild-type cells. We propose that a broken chromosome end can invade a homologous template in the absence of RAD51 and initiate DNA replication that may extend to the telomere, 100 or more kb away. Such break-induced replication appears to be similar to recombination-initiated replication in bacteria. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/8692957/Double_strand_break_repair_in_the_absence_of_RAD51_in_yeast:_a_possible_role_for_break_induced_DNA_replication_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=8692957 DB - PRIME DP - Unbound Medicine ER -