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Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis.
Infect Immun 1996; 64(7):2490-9II

Abstract

Brucella group 3 antigens (Ags) are outer membrane proteins (OMPs) with a molecular mass ranging from 25 to 30 kDa. The OMPs are of interest partially because of their potential use as vaccine and diagnostic reagents. We used human convalescent antibody (Ab) to clone a gene that encoded a 28-kDa protein from a lambdagt11 library of Brucella melitensis 16M genomic DNA. DNA sequence analysis revealed a single open reading frame that would encode a protein of 26,552 Da. The 28-kDa protein had a primary amino acid sequence that was 43% similar to a previously described Brucella abortus group 3 Ag, Omp25 (P. de Wergifosse, P. Lintermans, J. N. Limet, and A. Cloeckaert, J. Bacteriol. 177:1911-1914, 1995). The similarity to a known group 3 OMP, immunoreactivity with Ab prepared against B. abortus group Ags, immunolabeling of whole cells, and Southern hybridization led to our conclusion that the B. melitensis 28-kDa protein was a group 3 protein distinct from B. abortus Omp25. We designated the B. melitensis protein Omp28. Human convalescent sera from patients infected with B. abortus and Brucella suis as well as rabbit antisera prepared against killed B. abortus whole cells recognized B. melitensis Omp28 on Western blots (immunoblots). Furthermore, mice and goats infected with smooth strains of B. melitensis produced Abs against Omp28. Our results may begin to explain the variability in molecular weight seen in Brucella group Ags and point toward their possible use in vaccination against infection as well as diagnosis of the disease.

Authors+Show Affiliations

Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

8698471

Citation

Lindler, L E., et al. "Cloning of a Brucella Melitensis Group 3 Antigen Gene Encoding Omp28, a Protein Recognized By the Humoral Immune Response During Human Brucellosis." Infection and Immunity, vol. 64, no. 7, 1996, pp. 2490-9.
Lindler LE, Hadfield TL, Tall BD, et al. Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. Infect Immun. 1996;64(7):2490-9.
Lindler, L. E., Hadfield, T. L., Tall, B. D., Snellings, N. J., Rubin, F. A., Van De Verg, L. L., ... Warren, R. L. (1996). Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. Infection and Immunity, 64(7), pp. 2490-9.
Lindler LE, et al. Cloning of a Brucella Melitensis Group 3 Antigen Gene Encoding Omp28, a Protein Recognized By the Humoral Immune Response During Human Brucellosis. Infect Immun. 1996;64(7):2490-9. PubMed PMID: 8698471.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. AU - Lindler,L E, AU - Hadfield,T L, AU - Tall,B D, AU - Snellings,N J, AU - Rubin,F A, AU - Van De Verg,L L, AU - Hoover,D, AU - Warren,R L, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - 2490 EP - 9 JF - Infection and immunity JO - Infect. Immun. VL - 64 IS - 7 N2 - Brucella group 3 antigens (Ags) are outer membrane proteins (OMPs) with a molecular mass ranging from 25 to 30 kDa. The OMPs are of interest partially because of their potential use as vaccine and diagnostic reagents. We used human convalescent antibody (Ab) to clone a gene that encoded a 28-kDa protein from a lambdagt11 library of Brucella melitensis 16M genomic DNA. DNA sequence analysis revealed a single open reading frame that would encode a protein of 26,552 Da. The 28-kDa protein had a primary amino acid sequence that was 43% similar to a previously described Brucella abortus group 3 Ag, Omp25 (P. de Wergifosse, P. Lintermans, J. N. Limet, and A. Cloeckaert, J. Bacteriol. 177:1911-1914, 1995). The similarity to a known group 3 OMP, immunoreactivity with Ab prepared against B. abortus group Ags, immunolabeling of whole cells, and Southern hybridization led to our conclusion that the B. melitensis 28-kDa protein was a group 3 protein distinct from B. abortus Omp25. We designated the B. melitensis protein Omp28. Human convalescent sera from patients infected with B. abortus and Brucella suis as well as rabbit antisera prepared against killed B. abortus whole cells recognized B. melitensis Omp28 on Western blots (immunoblots). Furthermore, mice and goats infected with smooth strains of B. melitensis produced Abs against Omp28. Our results may begin to explain the variability in molecular weight seen in Brucella group Ags and point toward their possible use in vaccination against infection as well as diagnosis of the disease. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/8698471/Cloning_of_a_Brucella_melitensis_group_3_antigen_gene_encoding_Omp28_a_protein_recognized_by_the_humoral_immune_response_during_human_brucellosis_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=8698471 DB - PRIME DP - Unbound Medicine ER -