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Effect of herbimycin A on renal cancer cell growth.
Nihon Jinzo Gakkai Shi. 1996 May; 38(5):198-201.NJ

Abstract

We investigated the effect of herbimycin A on the monolayer growth of 4 human renal cell carcinoma (RCC) cell lines and a normal renal tubular cell line (RTC 13) using MTT [3-(4,5-dimethylthiszol-2,5-diphenyl tetrazolium bromide)] assay. Herbimycin A induced remarkable growth inhibition in each RCC cell line tested, without any morphological changes of the cells. At the concentration of 500 ng/ml, herbimycin A caused more than a 30% growth inhibition in all RCC cells (p < 0.005 vs RTC 13), while less than 7% growth inhibition was observed in RTC 13 at the same herbimycin A concentration. The cell cycle was estimated by analysing DNA (deoxyribonucleic acid) content using a FACS. A DNA histogram of RCC cells treated at herbimycin A showed a block in the cell cycle at the S and G2M phases. However, little effect by herbimycin A on RTC 13 cells was observed. Our results suggest that protein tyrosine kinases inhibitors, like herbimycin A, may offer a new treatment option for RCC patients.

Authors+Show Affiliations

Department of Urology, National Defense Medical College, Tokorozawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8699609

Citation

Tsuji, A, et al. "Effect of Herbimycin a On Renal Cancer Cell Growth." Nihon Jinzo Gakkai Shi, vol. 38, no. 5, 1996, pp. 198-201.
Tsuji A, Asano T, Suzuki S, et al. Effect of herbimycin A on renal cancer cell growth. Nihon Jinzo Gakkai Shi. 1996;38(5):198-201.
Tsuji, A., Asano, T., Suzuki, S., Aoyagi, T., Hayakawa, M., & Nakamura, H. (1996). Effect of herbimycin A on renal cancer cell growth. Nihon Jinzo Gakkai Shi, 38(5), 198-201.
Tsuji A, et al. Effect of Herbimycin a On Renal Cancer Cell Growth. Nihon Jinzo Gakkai Shi. 1996;38(5):198-201. PubMed PMID: 8699609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of herbimycin A on renal cancer cell growth. AU - Tsuji,A, AU - Asano,T, AU - Suzuki,S, AU - Aoyagi,T, AU - Hayakawa,M, AU - Nakamura,H, PY - 1996/5/1/pubmed PY - 1996/5/1/medline PY - 1996/5/1/entrez SP - 198 EP - 201 JF - Nihon Jinzo Gakkai shi JO - Nihon Jinzo Gakkai Shi VL - 38 IS - 5 N2 - We investigated the effect of herbimycin A on the monolayer growth of 4 human renal cell carcinoma (RCC) cell lines and a normal renal tubular cell line (RTC 13) using MTT [3-(4,5-dimethylthiszol-2,5-diphenyl tetrazolium bromide)] assay. Herbimycin A induced remarkable growth inhibition in each RCC cell line tested, without any morphological changes of the cells. At the concentration of 500 ng/ml, herbimycin A caused more than a 30% growth inhibition in all RCC cells (p < 0.005 vs RTC 13), while less than 7% growth inhibition was observed in RTC 13 at the same herbimycin A concentration. The cell cycle was estimated by analysing DNA (deoxyribonucleic acid) content using a FACS. A DNA histogram of RCC cells treated at herbimycin A showed a block in the cell cycle at the S and G2M phases. However, little effect by herbimycin A on RTC 13 cells was observed. Our results suggest that protein tyrosine kinases inhibitors, like herbimycin A, may offer a new treatment option for RCC patients. SN - 0385-2385 UR - https://www.unboundmedicine.com/medline/citation/8699609/Effect_of_herbimycin_A_on_renal_cancer_cell_growth_ L2 - http://www.diseaseinfosearch.org/result/3993 DB - PRIME DP - Unbound Medicine ER -